Abstract
The early membrane events taking place during mast cell secretion were followed in transmission and freeze-fracture electron microscopy. In order to slow down exocytosis and capture intermediate stages of membrane fusion, special conditions of incubation and stimulation were used. These were as follows: (a) the use of incubation media with altered ionic composition, and (b) stimulation with a low dosage of polymyxin B sulfate (4 microgram/ml) at low temperature (18 degrees C) for very short incubation times (30-60 s), with or without the presence of formaldehyde (0.8%). Under these conditions, unetchable circular impressions are found on the E face of the plasma membrane, 80-100 nm in diameter, with particles associated with their perimeters. In granule-to-granule fusion, the zone involved is demarcated by one or two rows of particles on the E face. In addition, raised circular areas of varying diameters (43-87 nm) surrounded by similar particles, also found on the E face, may represent potential sites before completion of fusion. Neither the circular impressions on the plasma membrane nor the sites on the granule membrane are permanent, but their appearance coincides with initiation of membrane fusion.
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Selected References
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- Anderson P., Slorach S. A., Uvnäs B. Sequential exocytosis of storage granules during antigen-induced histamine release from sensitized rat mast cells in vitro. An electron microscopic study. Acta Physiol Scand. 1973 Jul;88(3):359–372. doi: 10.1111/j.1748-1716.1973.tb05465.x. [DOI] [PubMed] [Google Scholar]
- Bach M. K., Bloch K. J., Austen K. F. IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. I. Optimum conditions for in vitro preparation of target cells with antibody and and challenge with antigen. J Exp Med. 1971 Apr 1;133(4):752–771. doi: 10.1084/jem.133.4.752. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beisson J., Lefort-Tran M., Pouphile M., Rossignol M., Satir B. Genetic analysis of membrane differentiation in Paramecium. Freeze-fracture study of the trichocyst cycle in wild-type and mutant strains. J Cell Biol. 1976 Apr;69(1):126–143. doi: 10.1083/jcb.69.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Branton D. Fracture faces of frozen membranes. Proc Natl Acad Sci U S A. 1966 May;55(5):1048–1056. doi: 10.1073/pnas.55.5.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burwen S. J., Rothman S. S. Zymogen granules: osmotic properties, interactions with ions, and some structural implications. Am J Physiol. 1972 May;222(5):1177–1181. doi: 10.1152/ajplegacy.1972.222.5.1177. [DOI] [PubMed] [Google Scholar]
- Chi E. Y., Lagunoff D., Koehler J. K. Electron microscopy of freeze-fractured rat peritoneal mast cells. J Ultrastruct Res. 1975 Apr;51(1):46–54. doi: 10.1016/s0022-5320(75)80007-4. [DOI] [PubMed] [Google Scholar]
- Chi E. Y., Lagunoff D., Koehler J. K. Freeze-fracture study of mast cell secretion. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2823–2827. doi: 10.1073/pnas.73.8.2823. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Douglas W. W. Involvement of calcium in exocytosis and the exocytosis--vesiculation sequence. Biochem Soc Symp. 1974;(39):1–28. [PubMed] [Google Scholar]
- Heuser J. E., Reese T. S., Landis D. M. Functional changes in frog neuromuscular junctions studied with freeze-fracture. J Neurocytol. 1974 Mar;3(1):109–131. doi: 10.1007/BF01111936. [DOI] [PubMed] [Google Scholar]
- Kessler S., Kuhn C. Scanning electron microscopy of mast cell degranulation. Lab Invest. 1975 Jan;32(1):71–77. [PubMed] [Google Scholar]
- Lagunoff D., Wan H. Temperature dependence of mast cell histamine secretion. J Cell Biol. 1974 Jun;61(3):809–811. doi: 10.1083/jcb.61.3.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Palade G. E., Bruns R. R. Structural modulations of plasmalemmal vesicles. J Cell Biol. 1968 Jun;37(3):633–649. doi: 10.1083/jcb.37.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Röhlich P., Anderson P., Uvnäs B. Electron microscope observations on compounds 48-80-induced degranulation in rat mast cells. Evidence for sequential exocytosis of storage granules. J Cell Biol. 1971 Nov;51(21):465–483. doi: 10.1083/jcb.51.2.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Satir B. Membrane events during the secretory process. Symp Soc Exp Biol. 1974;(28):399–418. [PubMed] [Google Scholar]
- Satir B., Schooley C., Satir P. Membrane fusion in a model system. Mucocyst secretion in Tetrahymena. J Cell Biol. 1973 Jan;56(1):153–176. doi: 10.1083/jcb.56.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tillack T. W., Marchesi V. T. Demonstration of the outer surface of freeze-etched red blood cell membranes. J Cell Biol. 1970 Jun;45(3):649–653. doi: 10.1083/jcb.45.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tizard I. R., Holmes W. L. Degranulation of sensitised rat peritoneal mast cells in response to antigen, compound 48-80 and polymyxin B. A scanning electron microscope study. Int Arch Allergy Appl Immunol. 1974;46(6):867–879. doi: 10.1159/000231189. [DOI] [PubMed] [Google Scholar]