Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1980 Jun 1;85(3):881–889. doi: 10.1083/jcb.85.3.881

Nucleus-associated intermediate filaments from chicken erythrocytes

PMCID: PMC2111451  PMID: 7190151

Abstract

Chicken erythrocyte nuclei prepared by isolation in isotonic KCl and Nonidet P-40 detergent were found to contain numerous attached filaments with a mean diameter of 11.0 nm. In polypeptide content and solubility properties, they resembled the vimentin type of intermediate filament found in cells of mesenchymal origin. Examination of their association with the nucleus suggests that more than a simple membrane attachment is involved.

Full Text

The Full Text of this article is available as a PDF (997.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett G. S., Fellini S. A., Croop J. M., Otto J. J., Bryan J., Holtzer H. Differences among 100-A filamentilament subunits from different cell types. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4364–4368. doi: 10.1073/pnas.75.9.4364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blose S. H., Chacko S. Rings of intermediate (100 A) filament bundles in the perinuclear region of vascular endothelial cells. Their mobilization by colcemid and mitosis. J Cell Biol. 1976 Aug;70(2 Pt 1):459–466. doi: 10.1083/jcb.70.2.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blose S. H., Shelanski M. L., Chacko S. Localization of bovine brain filament antibody on intermediate (100 A) filaments in guinea pig vascular endothelial cells and chick cardiac muscle cells. Proc Natl Acad Sci U S A. 1977 Feb;74(2):662–665. doi: 10.1073/pnas.74.2.662. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davison P. F., Hong B. S., Cooke P. Classes of distinguishable 10 nm cytoplasmic filaments. Exp Cell Res. 1977 Oct 15;109(2):471–474. doi: 10.1016/0014-4827(77)90033-7. [DOI] [PubMed] [Google Scholar]
  5. Drochmans P., Freudenstein C., Wanson J. C., Laurent L., Keenan T. W., Stadler J., Leloup R., Franke W. W. Structure and biochemical composition of desmosomes and tonofilaments isolated from calf muzzle epidermis. J Cell Biol. 1978 Nov;79(2 Pt 1):427–443. doi: 10.1083/jcb.79.2.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Franke W. W., Appelhans B., Schmid E., Freudenstein C., Osborn M., Weber K. The organization of cytokeratin filaments in the intestinal epithelium. Eur J Cell Biol. 1979 Aug;19(3):255–268. [PubMed] [Google Scholar]
  7. Franke W. W., Grund C., Osborn M., Weber K. The intermediate-sized filaments in rat kangaroo PtK2 cells. I. Morphology in situ. Cytobiologie. 1978 Aug;17(2):365–391. [PubMed] [Google Scholar]
  8. Franke W. W., Grund C., Schmid E. Intermediate-sized filaments present in Sertoli cells are of the vimentin type. Eur J Cell Biol. 1979 Aug;19(3):269–275. [PubMed] [Google Scholar]
  9. Franke W. W. Relationship of nuclear membranes with filaments and microtubules. Protoplasma. 1971;73(2):263–292. doi: 10.1007/BF01275600. [DOI] [PubMed] [Google Scholar]
  10. Franke W. W., Schmid E., Breitkreutz D., Lüder M., Boukamp P., Fusenig N. E., Osborn M., Weber K. Simultaneous expression of two different types of intermediate sized filaments in mouse keratinocytes proliferating in vitro. Differentiation. 1979;14(1-2):35–50. doi: 10.1111/j.1432-0436.1979.tb01010.x. [DOI] [PubMed] [Google Scholar]
  11. Franke W. W., Schmid E., Osborn M., Weber K. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5034–5038. doi: 10.1073/pnas.75.10.5034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Franke W. W., Schmid E., Osborn M., Weber K. Intermediate-sized filaments of human endothelial cells. J Cell Biol. 1979 Jun;81(3):570–580. doi: 10.1083/jcb.81.3.570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Franke W. W., Schmid E., Osborn M., Weber K. The intermediate-sized filaments in rat kangaroo PtK2 cells. II. Structure and composition of isolated filaments. Cytobiologie. 1978 Aug;17(2):392–411. [PubMed] [Google Scholar]
  14. Franke W. W., Schmid E., Weber K., Osborn M. HeLa cells contain intermediate-sized filaments of the prekeratin type. Exp Cell Res. 1979 Jan;118(1):95–109. doi: 10.1016/0014-4827(79)90587-1. [DOI] [PubMed] [Google Scholar]
  15. Franke W. W., Schmid E., Winter S., Osborn M., Weber K. Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates. Exp Cell Res. 1979 Oct 1;123(1):25–46. doi: 10.1016/0014-4827(79)90418-x. [DOI] [PubMed] [Google Scholar]
  16. Franke W. W., Weber K., Osborn M., Schmid E., Freudenstein C. Antibody to prekeratin. Decoration of tonofilament like arrays in various cells of epithelial character. Exp Cell Res. 1978 Oct 15;116(2):429–445. doi: 10.1016/0014-4827(78)90466-4. [DOI] [PubMed] [Google Scholar]
  17. Gordon W. E., 3rd, Bushnell A., Burridge K. Characterization of the intermediate (10 nm) filaments of cultured cells using an autoimmune rabbit antiserum. Cell. 1978 Feb;13(2):249–261. doi: 10.1016/0092-8674(78)90194-0. [DOI] [PubMed] [Google Scholar]
  18. Harris J. R., Brown J. N. Fractionation of the avian erythrocyte: an ultrastructural study. J Ultrastruct Res. 1971 Jul;36(1):8–23. doi: 10.1016/s0022-5320(71)80085-0. [DOI] [PubMed] [Google Scholar]
  19. Harris J. R. The preparation and ultrastructure of avian erythrocyte nuclear envelope enclosed by the plasma membrane. J Cell Sci. 1978 Dec;34:81–90. doi: 10.1242/jcs.34.1.81. [DOI] [PubMed] [Google Scholar]
  20. Hubbard B. D., Lazarides E. Copurification of actin and desmin from chicken smooth muscle and their copolymerization in vitro to intermediate filaments. J Cell Biol. 1979 Jan;80(1):166–182. doi: 10.1083/jcb.80.1.166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hynes R. O., Destree A. T. 10 nm filaments in normal and transformed cells. Cell. 1978 Jan;13(1):151–163. doi: 10.1016/0092-8674(78)90146-0. [DOI] [PubMed] [Google Scholar]
  22. Ishikawa H., Bischoff R., Holtzer H. Mitosis and intermediate-sized filaments in developing skeletal muscle. J Cell Biol. 1968 Sep;38(3):538–555. doi: 10.1083/jcb.38.3.538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Kurki P., Linder E., Virtanen I., Stenman S. Human smooth muscle autoantibodies reacting with intermediate (100 A) filaments. Nature. 1977 Jul 21;268(5617):240–241. doi: 10.1038/268240a0. [DOI] [PubMed] [Google Scholar]
  24. LaFountain J. R., Jr, Zobel C. R., Thomas H. R., Galbreath C. Fixation and staining of F-actin and microfilaments using tannic acid. J Ultrastruct Res. 1977 Jan;58(1):78–86. doi: 10.1016/s0022-5320(77)80009-9. [DOI] [PubMed] [Google Scholar]
  25. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  26. Lazarides E., Hubbard B. D. Immunological characterization of the subunit of the 100 A filaments from muscle cells. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4344–4348. doi: 10.1073/pnas.73.12.4344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Lazarides E. The distribution of desmin (100 A) filaments in primary cultures of embryonic chick cardiac cells. Exp Cell Res. 1978 Mar 15;112(2):265–273. doi: 10.1016/0014-4827(78)90209-4. [DOI] [PubMed] [Google Scholar]
  28. Lehto V. P., Virtanen I., Kurki P. Intermediate filaments anchor the nuclei in nuclear monolayers of cultured human fibroblasts. Nature. 1978 Mar 9;272(5649):175–177. doi: 10.1038/272175a0. [DOI] [PubMed] [Google Scholar]
  29. Liem R. K., Yen S. H., Salomon G. D., Shelanski M. L. Intermediate filaments in nervous tissues. J Cell Biol. 1978 Dec;79(3):637–645. doi: 10.1083/jcb.79.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Osborn M., Franke W. W., Weber K. Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (Pt K2) by immunofluorescence microscopy. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2490–2494. doi: 10.1073/pnas.74.6.2490. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Small J. V., Celis J. E. Direct visualization of the 10-nm (100-A)-filament network in whole and enucleated cultured cells. J Cell Sci. 1978 Jun;31:393–409. doi: 10.1242/jcs.31.1.393. [DOI] [PubMed] [Google Scholar]
  32. Small J. V., Sobieszek A. Studies on the function and composition of the 10-NM(100-A) filaments of vertebrate smooth muscle. J Cell Sci. 1977 Feb;23:243–268. doi: 10.1242/jcs.23.1.243. [DOI] [PubMed] [Google Scholar]
  33. Starger J. M., Brown W. E., Goldman A. E., Goldman R. D. Biochemical and immunological analysis of rapidly purified 10-nm filaments from baby hamster kidney (BHK-21) cells. J Cell Biol. 1978 Jul;78(1):93–109. doi: 10.1083/jcb.78.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Starger J. M., Goldman R. D. Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2422–2426. doi: 10.1073/pnas.74.6.2422. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Steinert P. M., Idler W. W., Zimmerman S. B. Self-assembly of bovine epidermal keratin filaments in vitro. J Mol Biol. 1976 Dec 15;108(3):547–567. doi: 10.1016/s0022-2836(76)80136-2. [DOI] [PubMed] [Google Scholar]
  36. Steinert P. M. Structure of the three-chain unit of the bovine epidermal keratin filament. J Mol Biol. 1978 Jul 25;123(1):49–70. doi: 10.1016/0022-2836(78)90376-5. [DOI] [PubMed] [Google Scholar]
  37. Steinert P. M., Zimmerman S. B., Starger J. M., Goldman R. D. Ten-nanometer filaments of hamster BHK-21 cells and epidermal keratin filaments have similar structures. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6098–6101. doi: 10.1073/pnas.75.12.6098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Virtanen I., Kurkinen M., Lehto V. P. Nucleus-anchoring cytoskeleton in chicken red blood cells. Cell Biol Int Rep. 1979 Mar;3(2):157–162. doi: 10.1016/0309-1651(79)90121-8. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES