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. 1988 Jun;170(6):2419–2426. doi: 10.1128/jb.170.6.2419-2426.1988

Systemic virulence of Erwinia chrysanthemi 3937 requires a functional iron assimilation system.

C Enard 1, A Diolez 1, D Expert 1
PMCID: PMC211150  PMID: 3372473

Abstract

In Erwinia chrysanthemi, conditions of iron starvation initiate production of a catechol-type siderophore and enhance production of three outer membrane polypeptides. Twenty-two mutants affected in the different stages of this iron assimilation system were isolated by mini-Mu insertion mutagenesis. All of them failed to induce systemic soft rot on axenically grown Saintpaulia plants. From the siderophore auxotrophs and the iron uptake mutants, clones having recovered the missing function(s) were isolated by using the in vivo cloning vector pULB113 (RP4::mini-Mu). An R-prime plasmid containing a ca. 35.5-kilobase-pair DNA insert was identified. Restoration of the iron functions restored partially, if not completely, the virulence of the parental strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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