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. 1980 Aug 1;86(2):624–633. doi: 10.1083/jcb.86.2.624

Immunocytochemical localization of coated vesicle protein in rodent nervous system

PMCID: PMC2111507  PMID: 6995469

Abstract

Immunocytochemistry has been used to study the distribution of the major 180,000-mol wt protein of coated vesicles in rodent cerebellum. An antibody to the coat protein was prepared in rabbits and characterized by immunodiffusion and immunofixation of polyacrylamide gels. At the light microscope level the protein was primarily localized in punctate profiles surrounding Purkinje cells and within the cerebellar glomeruli. At the electron microscope level the punctate distribution was confined to presynaptic terminals of basket and Golgi II neurons as well as mossy fiber terminals of the glomeruli. This label was heaviest on the lattice coat of coated vesicles but, in addition, label was found within the presynaptic axoplasm and along the cytoplasmic surface of the plasmalemma. Coated vesicles in cell somata were labeled as well as the cytosol around groupings of these vesicles. These data suggest that there may be two forms (or more) of coated vesicle protein in neurons, a lattice form associated with coated vesicles and a soluble form associated with the cytoplasmic matrix.

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Selected References

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  1. Adair W. S., Jurivich D., Goodenough U. W. Localization of cellular antigens in sodium dodecyl sulfate-polyacrylamide gels. J Cell Biol. 1978 Oct;79(1):281–285. doi: 10.1083/jcb.79.1.281. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson R. G., Brown M. S., Goldstein J. L. Role of the coated endocytic vesicle in the uptake of receptor-bound low density lipoprotein in human fibroblasts. Cell. 1977 Mar;10(3):351–364. doi: 10.1016/0092-8674(77)90022-8. [DOI] [PubMed] [Google Scholar]
  3. Anderson R. G., Vasile E., Mello R. J., Brown M. S., Goldstein J. L. Immunocytochemical visualization of coated pits and vesicles in human fibroblasts: relation to low density lipoprotein receptor distribution. Cell. 1978 Nov;15(3):919–933. doi: 10.1016/0092-8674(78)90276-3. [DOI] [PubMed] [Google Scholar]
  4. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  5. Blitz A. L., Fine R. E., Toselli P. A. Evidence that coated vesicles isolated from brain are calcium-sequestering organelles resembling sarcoplasmic reticulum. J Cell Biol. 1977 Oct;75(1):135–147. doi: 10.1083/jcb.75.1.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  7. Fine R. E., Blitz A. L., Sack D. H. Characterization of anti-clathrin serum. FEBS Lett. 1978 Oct 1;94(1):59–62. doi: 10.1016/0014-5793(78)80906-5. [DOI] [PubMed] [Google Scholar]
  8. Franke W. W., Lüder M. R., Kartenbeck J., Zerban H., Keenan T. W. Involvement of vesicle coat material in casein secretion and surface regeneration. J Cell Biol. 1976 Apr;69(1):173–195. doi: 10.1083/jcb.69.1.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Friend D. S., Farquhar M. G. Functions of coated vesicles during protein absorption in the rat vas deferens. J Cell Biol. 1967 Nov;35(2):357–376. doi: 10.1083/jcb.35.2.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Heuser J. E., Reese T. S. Evidence for recycling of synaptic vesicle membrane during transmitter release at the frog neuromuscular junction. J Cell Biol. 1973 May;57(2):315–344. doi: 10.1083/jcb.57.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mendell J. R., Whitaker J. N., Engel W. K. The skeletal muscle binding site of antistriated muscle antibody in myasthenia gravis: an electron microscopic immunohistochemical study using peroxidase conjugated antibody fragments. J Immunol. 1973 Sep;111(3):847–856. [PubMed] [Google Scholar]
  12. Mendell J. R., Whitaker J. N. Immunocytochemical localization studies of myelin basic protein. J Cell Biol. 1978 Feb;76(2):502–511. doi: 10.1083/jcb.76.2.502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Novikoff A. B., Novikoff P. M., Quintana N., Davis C. Diffusion artificats in 3,3'-diaminobenzidine cytochemistry. J Histochem Cytochem. 1972 Sep;20(9):745–749. doi: 10.1177/20.9.745. [DOI] [PubMed] [Google Scholar]
  14. Pearse B. M. Coated vesicles from pig brain: purification and biochemical characterization. J Mol Biol. 1975 Sep 5;97(1):93–98. doi: 10.1016/s0022-2836(75)80024-6. [DOI] [PubMed] [Google Scholar]
  15. ROTH T. F., PORTER K. R. YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L. J Cell Biol. 1964 Feb;20:313–332. doi: 10.1083/jcb.20.2.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rees R. P., Bunge M. B., Bunge R. P. Morphological changes in the neuritic growth cone and target neuron during synaptic junction development in culture. J Cell Biol. 1976 Feb;68(2):240–263. doi: 10.1083/jcb.68.2.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Roth T. F., Cutting J. A., Atlas S. B. Protein transport: a selective membrane mechanism. J Supramol Struct. 1976;4(4):527–548. doi: 10.1002/jss.400040413. [DOI] [PubMed] [Google Scholar]
  18. Wood J. G., Wallace R. W., Whitaker J. N., Cheung W. Y. Immunocytochemical localization of calmodulin and a heat-labile calmodulin-binding protein (CaM-BP80) in basal ganglia of mouse brain. J Cell Biol. 1980 Jan;84(1):66–76. doi: 10.1083/jcb.84.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]

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