Abstract
The osmotic regulation of the biosynthesis of membrane-derived oligosaccharides (MDO) in strains UB1005 and DC2 of Escherichia coli K-12 was examined; this regulation was previously reported by Clark (J. Bacteriol. 161:1049-1053, 1985) to be different from that observed by Kennedy for other strains of E. coli (Proc. Natl. Acad. Sci. USA 79:1092-1095, 1982). Osmotic regulation of the synthesis of MDO in UB1005 and DC2 is in fact indistinguishable from that previously reported for other strains of E. coli, with maximum production of MDO occurring in the medium of lowest osmolarity. The report of Clark to the contrary was apparently based on the inadequate methods for the measurement of MDO employed in that study. MDO are localized in the periplasm of wild-type E. coli cells. However, strain DC2, selected for hypersensitivity to a range of antibiotics, released most of its MDO into the medium, apparently as a result of greater outer membrane permeability.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Clark D. P. Mutant of Escherichia coli deficient in osmoregulation of periplasmic oligosaccharide synthesis. J Bacteriol. 1985 Mar;161(3):1049–1053. doi: 10.1128/jb.161.3.1049-1053.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kennedy E. P. Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1092–1095. doi: 10.1073/pnas.79.4.1092. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kennedy E. P., Rumley M. K., Schulman H., Van Golde L. M. Identification of sn-glycero-1-phosphate and phosphoethanolamine residues linked to the membrane-derived Oligosaccharides of Escherichia coli. J Biol Chem. 1976 Jul 25;251(14):4208–4213. [PubMed] [Google Scholar]
- Miller K. J., Kennedy E. P., Reinhold V. N. Osmotic adaptation by gram-negative bacteria: possible role for periplasmic oligosaccharides. Science. 1986 Jan 3;231(4733):48–51. doi: 10.1126/science.3941890. [DOI] [PubMed] [Google Scholar]
- Munro G. F., Bell C. A. Effects of external osmolarity on phospholipid metabolism in Escherichia coli B. J Bacteriol. 1973 Oct;116(1):257–262. doi: 10.1128/jb.116.1.257-262.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raetz C. R., Newman K. F. Diglyceride kinase mutants of Escherichia coli: inner membrane association of 1,2-diglyceride and its relation to synthesis of membrane-derived oligosaccharides. J Bacteriol. 1979 Feb;137(2):860–868. doi: 10.1128/jb.137.2.860-868.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raetz C. R., Newman K. F. Neutral lipid accumulation in the membranes of Escherichia coli mutants lacking diglyceride kinase. J Biol Chem. 1978 Jun 10;253(11):3882–3887. [PubMed] [Google Scholar]
- Roller S. D., Anagnostopoulos G. D. Accumulation of carbohydrate by Escherichia coli B/r/1 during growth at low water activity. J Appl Bacteriol. 1982 Jun;52(3):425–434. doi: 10.1111/j.1365-2672.1982.tb05073.x. [DOI] [PubMed] [Google Scholar]
- Schulman H., Kennedy E. P. Localization of membrane-derived oligosaccharides in the outer envelope of Escherichia coli and their occurrence in other Gram-negative bacteria. J Bacteriol. 1979 Jan;137(1):686–688. doi: 10.1128/jb.137.1.686-688.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Golde L. M. Metabolism of membrane phospholipids and its relation to a novel class of oligosaccharides in Escherichia coli. Proc Natl Acad Sci U S A. 1973 May;70(5):1368–1372. doi: 10.1073/pnas.70.5.1368. [DOI] [PMC free article] [PubMed] [Google Scholar]