Abstract
We have examined the ribosomal structural proteins isolated from vegetatively growing Tetrahymena pyriformis and from cells that had been starved of all nutrients for 24 h. Reproducible, nonartifactual differences in protein complement, primarily associated with the large ribosomal subunit, were found. The kinetics of change in ribosomal protein complement were followed both in refed and in newly starved cells. Furthermore, attempts at correlating a certain protein "phenotype" with a particular functional state of the ribosome were made. It was concluded that the alterations seen could not be correlated with a specific stage in the normal ribosome cycle. We did show, however, that the change in protein complement could occur as a result of altering preexisting ribosomes. In addition, we showed that the change correlates with a decrease in growth rate rather than being caused by the starvation conditions themselves. Speculations as to the functional significance of the protein changes are presented.
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- Conner R. L., Koroly M. J. Relationship of cellular energetics to RNA metabolism in Tetrahymena pyriformis W. J Protozool. 1974 Feb;21(1):177–182. doi: 10.1111/j.1550-7408.1974.tb03634.x. [DOI] [PubMed] [Google Scholar]
- Cooper H. L., Berger S. L., Braverman R. Free ribosomes in physiologically nondividing cells. Human peripheral lymphocytes. J Biol Chem. 1976 Aug 25;251(16):4891–4900. [PubMed] [Google Scholar]
- Hallberg R. L., Bruns P. J. Ribosome biosynthesis in Tetrahymena pyriformis. Regulation in response to nutritional changes. J Cell Biol. 1976 Nov;71(2):383–394. doi: 10.1083/jcb.71.2.383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henshaw E. C., Guiney D. G., Hirsch C. A. The ribosome cycle in mammalian protein synthesis. I. The place of monomeric ribosomes and ribosomal subunits in the cycle. J Biol Chem. 1973 Jun 25;248(12):4367–4376. [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Leighton T. Sporulation-specific translational discrimination in Bacillus subtilis. J Mol Biol. 1974 Jul 15;86(4):855–863. doi: 10.1016/0022-2836(74)90358-1. [DOI] [PubMed] [Google Scholar]
- Lovett J. S. Growth and differentiation of the water mold Blastocladiella emersonii: cytodifferentiation and the role of ribonucleic acid and protein synthesis. Bacteriol Rev. 1975 Dec;39(4):345–404. doi: 10.1128/br.39.4.345-404.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Szyszko A. H., Prazak B. L., Ehret C. F., Eisler W. J., Jr, Wille J. J., Jr A multi-unit sampling system and its use in the characterization of ultradian and infradian growth in Tetrahymena. J Protozool. 1968 Nov;15(4):781–785. doi: 10.1111/j.1550-7408.1968.tb02213.x. [DOI] [PubMed] [Google Scholar]
- Venrooij W. J., Janssen A. P. Heterogeneity of native ribosomal 60-S subunits in Ehrlich ascites tumor cells cultured in vitro. Eur J Biochem. 1976 Oct 1;69(1):55–60. doi: 10.1111/j.1432-1033.1976.tb10857.x. [DOI] [PubMed] [Google Scholar]
- Warner J. R., Udem S. A. Temperature sensitive mutations affecting ribosome synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):243–257. doi: 10.1016/0022-2836(72)90280-x. [DOI] [PubMed] [Google Scholar]
- Zinker S., Warner J. R. The ribosomal proteins of Saccharomyces cerevisiae. Phosphorylated and exchangeable proteins. J Biol Chem. 1976 Mar 25;251(6):1799–1807. [PubMed] [Google Scholar]