Abstract
The in vitro assembly of myosin purified from calf aorta muscle has been studied by electron microscopy. Two types of filament are formed: short bipolar filament similar to those formed from skeletal muscle myosin, and longer "side-polar" filaments having cross bridges with a single polarity along the entire length of one side and the opposite polarity along the other side. Unlike the case with skeletal myosin filaments, antiparallel interactions between myosin molecules occur along the whole length of side-polar filaments. The side-polar structure may be related to the in vivo form of myosin in vertebrate smooth muscle.
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Selected References
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- Adelstein R. S., Conti M. A., Johnson G. S., Pastan I., Pollard T. D. Isolation and characterization of myosin from cloned mouse fibroblasts. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3693–3697. doi: 10.1073/pnas.69.12.3693. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ashton F. T., Somlyo A. V., Somlyo A. P. The contractile apparatus of vascular smooth muscle: intermediate high voltage stereo electron microscopy. J Mol Biol. 1975 Oct 15;98(1):17–29. doi: 10.1016/s0022-2836(75)80098-2. [DOI] [PubMed] [Google Scholar]
- Elliott A., Offer G., Burridge K. Electron microscopy of myosin molecules from muscle and non-muscle sources. Proc R Soc Lond B Biol Sci. 1976 Mar 30;193(1110):45–53. doi: 10.1098/rspb.1976.0030. [DOI] [PubMed] [Google Scholar]
- Hinssen H., D'Haese J. Filament formation by slime mould myosin isolated at low ionic strength. J Cell Sci. 1974 Jun;15(1):113–129. doi: 10.1242/jcs.15.1.113. [DOI] [PubMed] [Google Scholar]
- Jones A. W., Somlyo A. P., Somlyo A. V. Potassium accumulation in smooth muscle and associated ultrastructural changes. J Physiol. 1973 Jul;232(2):247–273. doi: 10.1113/jphysiol.1973.sp010268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kendrick-Jones J., Szent-Gyorgyi A. S., Cohen C. Segments from vertebrate smooth muscle myosin rods. J Mol Biol. 1971 Aug 14;59(3):527–529. doi: 10.1016/0022-2836(71)90316-0. [DOI] [PubMed] [Google Scholar]
- Niederman R., Pollard T. D. Human platelet myosin. II. In vitro assembly and structure of myosin filaments. J Cell Biol. 1975 Oct;67(1):72–92. doi: 10.1083/jcb.67.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shoenberg C. F., Haselgrove J. C. Filaments and ribbons in vertebrate smooth muscle. Nature. 1974 May 10;249(453):152–154. doi: 10.1038/249152a0. [DOI] [PubMed] [Google Scholar]
- Small J. V., Squire J. M. Structural basis of contraction in vertebrate smooth muscle. J Mol Biol. 1972 Jun 14;67(1):117–149. doi: 10.1016/0022-2836(72)90390-7. [DOI] [PubMed] [Google Scholar]
- Small J. V. Studies on isolated smooth muscle cells: The contractile apparatus. J Cell Sci. 1977 Apr;24:327–349. doi: 10.1242/jcs.24.1.327. [DOI] [PubMed] [Google Scholar]
- Sobieszek A., Small J. V. The assembly of ribbon-shaped structures in low ionic strength extracts obtained from vertebrate smooth muscle. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):203–212. doi: 10.1098/rstb.1973.0024. [DOI] [PubMed] [Google Scholar]
- Somlyo A. P., Devine C. E., Somlyo A. V., Rice R. V. Filament organization in vertebrate smooth muscle. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):223–229. doi: 10.1098/rstb.1973.0027. [DOI] [PubMed] [Google Scholar]
- Somlyo A. P., Somlyo A. V., Devine C. E., Rice R. V. Aggregation of thick filaments into ribbons in mammalian smooth muscle. Nat New Biol. 1971 Jun 23;231(25):243–246. doi: 10.1038/newbio231243a0. [DOI] [PubMed] [Google Scholar]
- Squire J. M. Muscle filament structure and muscle contraction. Annu Rev Biophys Bioeng. 1975;4(00):137–163. doi: 10.1146/annurev.bb.04.060175.001033. [DOI] [PubMed] [Google Scholar]
- Wachsberger P. R., Pepe F. A. Purification of uterine myosin and synthetic filament formation. J Mol Biol. 1974 Sep 15;88(2):385–391. doi: 10.1016/0022-2836(74)90489-6. [DOI] [PubMed] [Google Scholar]