Abstract
Optical diffraction patterns from electron micrographs of both longitudinal and cross sections of normal and anomalous canine cardiac Z bands have been compared. The data indicate that anomalous cardiac Z bands resembling nemaline rods are structurally related to Z bands in showing a repeating lattice common to both. In thin sections transverse to the myofibril axis, both electron micrographs and optical diffraction patterns of the Z structure reveal a square lattice of 24 nm. This lattice is simple at the edge of each I band and centered in the interior of the Z band, where two distinct lattice forms have been observed. In longitudinal sections, oblique filaments visible in the electron micrographs correspond to a 38-nm axial periodicity in diffraction patterns of both Z band and Z rod. We conclude that the Z rods will be useful for further analysis and reconstruction of the Z lattice by optical diffraction techniques.
Full Text
The Full Text of this article is available as a PDF (5.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bishop S. P., Cole C. R. Ultrastructural changes in the canine myocardium with right ventricular hypertrophy and congestive heart failure. Lab Invest. 1969 Mar;20(3):219–229. [PubMed] [Google Scholar]
- Caspar D. L., Cohen C., Longley W. Tropomyosin: crystal structure, polymorphism and molecular interactions. J Mol Biol. 1969 Apr 14;41(1):87–107. doi: 10.1016/0022-2836(69)90128-4. [DOI] [PubMed] [Google Scholar]
- Engel A. G., Gomez M. R. Nemaline (Z disk) myopathy: observations on the origin, structure, and solubility properties of the nemaline structures. J Neuropathol Exp Neurol. 1967 Oct;26(4):601–619. doi: 10.1097/00005072-196710000-00007. [DOI] [PubMed] [Google Scholar]
- FRANZINI-ARMSTRONG C., PORTER K. R. THE Z DISC OF SKELETAL MUSCLE FIBRILS. Z Zellforsch Mikrosk Anat. 1964;61:661–672. doi: 10.1007/BF00342617. [DOI] [PubMed] [Google Scholar]
- Fawcett D. W. The sporadic occurrence in cardiac muscle of anomalous Z bands exhibiting a periodic structure suggestive of tropomyosin. J Cell Biol. 1968 Jan;36(1):266–270. doi: 10.1083/jcb.36.1.266. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HUXLEY H. E. ELECTRON MICROSCOPE STUDIES ON THE STRUCTURE OF NATURAL AND SYNTHETIC PROTEIN FILAMENTS FROM STRIATED MUSCLE. J Mol Biol. 1963 Sep;7:281–308. doi: 10.1016/s0022-2836(63)80008-x. [DOI] [PubMed] [Google Scholar]
- KNAPPEIS G. G., CARLSEN F. The ultrastructure of the Z disc in skeletal muscle. J Cell Biol. 1962 May;13:323–335. doi: 10.1083/jcb.13.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelly D. E., Cahill M. A. Filamentous and matrix components of skeletal muscle Z-disks. Anat Rec. 1972 Apr;172(4):623–642. doi: 10.1002/ar.1091720403. [DOI] [PubMed] [Google Scholar]
- Kelly D. E. Models of muscle Z-band fine structure based on a looping filament configuration. J Cell Biol. 1967 Sep;34(3):827–840. doi: 10.1083/jcb.34.3.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landon D. N. The influence of fixation upon the fine structure of the Z-disk of rat striated muscle. J Cell Sci. 1970 Jan;6(1):257–276. doi: 10.1242/jcs.6.1.257. [DOI] [PubMed] [Google Scholar]
- Legato M. J. Sarcomerogenesis in human myocardium. J Mol Cell Cardiol. 1970 Dec;1(4):425–437. doi: 10.1016/0022-2828(70)90039-8. [DOI] [PubMed] [Google Scholar]
- Millman B. M., Bennett P. M., Bennett P. M. Structure of the cross-striated adductor muscle of the scallop. J Mol Biol. 1976 May 25;103(3):439–467. doi: 10.1016/0022-2836(76)90212-6. [DOI] [PubMed] [Google Scholar]
- O'Brien E. J., Bennett P. M., Hanson J. Optical diffraction studies of myofibrillar structure. Philos Trans R Soc Lond B Biol Sci. 1971 May 27;261(837):201–208. doi: 10.1098/rstb.1971.0051. [DOI] [PubMed] [Google Scholar]
- Rowe R. W., Morton D. J. Faults in the square lattice of mammalian skeletal muscle Z-disks. J Cell Sci. 1971 Jul;9(1):139–145. doi: 10.1242/jcs.9.1.139. [DOI] [PubMed] [Google Scholar]
- Rowe R. W. The ultrastructure of Z disks from white, intermediate, and red fibers of mammalian striated muscles. J Cell Biol. 1973 May;57(2):261–277. doi: 10.1083/jcb.57.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe R. W. Ultrastructure of the Z line of skeletal muscle fibers. J Cell Biol. 1971 Dec;51(3):674–685. doi: 10.1083/jcb.51.3.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHY G. M., ENGEL W. K., SOMERS J. E., WANKO T. NEMALINE MYOPATHY. A NEW CONGENITAL MYOPATHY. Brain. 1963 Dec;86:793–810. doi: 10.1093/brain/86.4.793. [DOI] [PubMed] [Google Scholar]
- Stromer M. H., Tabatabai L. B., Robson R. M., Goll D. E., Zeece M. G. Nemaline myopathy, an integrated study: selective extraction. Exp Neurol. 1976 Feb;50(2):402–421. doi: 10.1016/0014-4886(76)90014-5. [DOI] [PubMed] [Google Scholar]