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. 1981 May 1;89(2):346–356. doi: 10.1083/jcb.89.2.346

Video image processing greatly enhances contrast, quality, and speed in polarization-based microscopy

PMCID: PMC2111689  PMID: 6788777

Abstract

Video cameras with contrast and black level controls can yield polarized light and differential interference contrast microscope images with unprecedented image quality, resolution, and recording speed. The theoretical basis and practical aspects of video polarization and differential interference contrast microscopy are discussed and several applications in cell biology are illustrated. These include: birefringence of cortical structures and beating cilia in Stentor, birefringence of rotating flagella on a single bacterium, growth and morphogenesis of echinoderm skeletal spicules in culture, ciliary and electrical activity in a balancing organ of a nudibranch snail, and acrosomal reaction in activated sperm.

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Selected References

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  1. Berns M. W. Partial cell irradiation with a tunable organic dye laser. Nature. 1972 Dec 22;240(5382):483–485. doi: 10.1038/240483a0. [DOI] [PubMed] [Google Scholar]
  2. Dvorak J. A., Miller L. H., Whitehouse W. C., Shiroishi T. Invasion of erythrocytes by malaria merozoites. Science. 1975 Feb 28;187(4178):748–750. doi: 10.1126/science.803712. [DOI] [PubMed] [Google Scholar]
  3. Erickson R. O. Tubular packing of spheres in biological fine structure. Science. 1973 Aug 24;181(4101):705–716. doi: 10.1126/science.181.4101.705. [DOI] [PubMed] [Google Scholar]
  4. Gilkey J. C., Jaffe L. F., Ridgway E. B., Reynolds G. T. A free calcium wave traverses the activating egg of the medaka, Oryzias latipes. J Cell Biol. 1978 Feb;76(2):448–466. doi: 10.1083/jcb.76.2.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. INOUE S., KUBOTA H. Diffraction anomaly in polarizing microscopes. Nature. 1958 Dec 20;182(4651):1725–1726. doi: 10.1038/1821725a0. [DOI] [PubMed] [Google Scholar]
  6. Inoué S., Ritter H., Jr Mitosis in Barbulanympha. II. Dynamics of a two-stage anaphase, nuclear morphogenesis, and cytokinesis. J Cell Biol. 1978 Jun;77(3):655–684. doi: 10.1083/jcb.77.3.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. KUBOTA H., INOUE S. [Diffraction images in the polarizing microscope]. J Opt Soc Am. 1959 Feb;49(2):191–198. doi: 10.1364/josa.49.000191. [DOI] [PubMed] [Google Scholar]
  8. Reynolds G. T. Image intensification applied to biological problems. Q Rev Biophys. 1972 Aug;5(3):295–347. doi: 10.1017/s0033583500000974. [DOI] [PubMed] [Google Scholar]
  9. Ritter H., Jr, Inoué S., Kubai D. Mitosis in Barbulanympha. I. Spindle structure, formation, and kinetochore engagement. J Cell Biol. 1978 Jun;77(3):638–654. doi: 10.1083/jcb.77.3.638. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rose B., Loewenstein W. R. Calcium ion distribution in cytoplasm visualised by aequorin: diffusion in cytosol restricted by energized sequestering. Science. 1975 Dec 19;190(4220):1204–1206. doi: 10.1126/science.1198106. [DOI] [PubMed] [Google Scholar]
  11. Sato H., Ellis G. W., Inoué S. Microtubular origin of mitotic spindle form birefringence. Demonstration of the applicability of Wiener's equation. J Cell Biol. 1975 Dec;67(3):501–517. doi: 10.1083/jcb.67.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stommel E. W., Stephens R. E., Alkon D. L. Motile statocyst cilia transmit rather than directly transduce mechanical stimuli. J Cell Biol. 1980 Dec;87(3 Pt 1):652–662. doi: 10.1083/jcb.87.3.652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Taylor D. L., Blinks J. R., Reynolds G. Contractile basis of ameboid movement. VII. Aequorin luminescence during ameboid movement, endocytosis, and capping. J Cell Biol. 1980 Aug;86(2):599–607. doi: 10.1083/jcb.86.2.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taylor D. L., Wang Y. L., Heiple J. M. Contractile basis of ameboid movement. VII. The distribution of fluorescently labeled actin in living amebas. J Cell Biol. 1980 Aug;86(2):590–598. doi: 10.1083/jcb.86.2.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tilney L. G., Kiehart D. P., Sardet C., Tilney M. Polymerization of actin. IV. Role of Ca++ and H+ in the assembly of actin and in membrane fusion in the acrosomal reaction of echinoderm sperm. J Cell Biol. 1978 May;77(2):536–550. doi: 10.1083/jcb.77.2.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Willingham M. C., Pastan I. The visualization of fluorescent proteins in living cells by video intensification microscopy (VIM). Cell. 1978 Mar;13(3):501–507. doi: 10.1016/0092-8674(78)90323-9. [DOI] [PubMed] [Google Scholar]

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