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. 1981 Apr 1;89(1):35–44. doi: 10.1083/jcb.89.1.35

Polarity of dynein-microtubule interactions in vitro: cross-bridging between parallel and antiparallel microtubules

PMCID: PMC2111773  PMID: 6453125

Abstract

Ciliary doublet microtubules produced by sliding disintegration in 20 muM MgATP2-reassociate in the presence of exogenous 30S dynein and 6 mM MgSO4. The doublets form overlapping arrays, held together by dynein cross-bridges. Dynein arms on both A and B subfibers serve as unambiguous markers of microtubule polarity within the arrays. Doublets reassociate via dynein cross-bridges in both parallel and antiparallel modes, although parallel interactions are favored 2:1. When 20 muM ATP is added to the arrays, the doublets undergo both vanadate-sensitive and insensitive forms of secondary disintegration to reproduce the original population of doublets. The results demonstrate that both parallel and antiparallel doublet cross-bridging is sensitive to dissociation by ATP even though normal ciliary motion depends strictly on dynein interactions between parallel microtubules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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