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. 1981 Aug 1;90(2):396–401. doi: 10.1083/jcb.90.2.396

Isoenzymes of lactate dehydrogenase in human leukemic cells in culture treated with inducers of differentiation

PMCID: PMC2111856  PMID: 6945307

Abstract

The human leukemic cell lines HL60 and K562, were induced to differentiate terminally by chemical agents. The isoenzyme patterns of lactate dehydrogenase (LD) in the cells before and after differentiation were determined electrophoretically on agarose gels. In general, treatment of the leukemic cells with inducers of differentiation resulted in a quantitative shift of the isoenzyme pattern towards anodic or cathodic forms. This was correlated with the conversion of the chemically treated cells to morphologically more normal cells, as verified by light microscopy and/or synthesis of hemoglobin. The LD isoenzyme patterns of the chemically differentiated cells were: (a) characteristic for the particular cell type obtained rather than for the nature of the inducer used; and (b) not similar to those of normally differentiated cells of the corresponding lineage, indicating that incomplete differentiation had occurred.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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