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. 1981 Aug 1;90(2):415–417. doi: 10.1083/jcb.90.2.415

Asynchronous appearance of newly synthesized histone H1 subfractions in HeLa chromatin

PMCID: PMC2111879  PMID: 7287813

Abstract

Incorporation of radioactive alanine into chromatin-bound subfractions of H1 histone was studied in HeLa cells synchronized by the double thymidine block technique. The subfractions were resolved into three chromatographic peaks by Biorex-70. In the period 5-7 h after release from the thymidine block, peaks I and III showed twice as much incorporation as they did in the period 1-3 h after release, whereas peak II showed three times the incorporation at 5-7 h that it did at 1- 3 h. Thus, the H1-histone subfraction in peak II appears in chromatin somewhat later in S phase than do the subfractions in Peaks I and III.

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Selected References

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