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The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1981 Aug 1;90(2):300–308. doi: 10.1083/jcb.90.2.300

Taxol induces postmitotic myoblasts to assemble interdigitating microtubule-myosin arrays that exclude actin filaments

PMCID: PMC2111880  PMID: 6116716

Abstract

Taxol has the following effects on myogenic cultures: (a) it blocks cell replication of presumptive myoblasts and fibroblasts. (b) It induces the aggregation of microtubules into sheets or massive cables in presumptive myoblasts and fibroblasts, but not in postmitotic, mononucleated myoblasts. (c) It induces normally elongated postmitotic myoblasts to form stubby, star-shaped cells. (d) It reversibly blocks the fusion of the star-shaped myoblasts into multinucleated myotubes. (e) It augments the number of microtubules in postmitotic myoblasts, and these are assembled into interdigitating arrays of microtubules and myosin filaments. (f) Actin filaments are largely excluded from these interdigitating microtubule-myosin complexes. (g) The myosin filaments in the interdigitating microtubule-myosin arrays are aligned laterally, forming A-bands approximately 1.5 micrometers long.

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Selected References

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  1. Bennett G. S., Fellini S. A., Croop J. M., Otto J. J., Bryan J., Holtzer H. Differences among 100-A filamentilament subunits from different cell types. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4364–4368. doi: 10.1073/pnas.75.9.4364. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett G. S., Fellini S. A., Toyama Y., Holtzer H. Redistribution of intermediate filament subunits during skeletal myogenesis and maturation in vitro. J Cell Biol. 1979 Aug;82(2):577–584. doi: 10.1083/jcb.82.2.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brinkley B. R., Fistel S. H., Marcum J. M., Pardue R. L. Microtubules in cultured cells; indirect immunofluorescent staining with tubulin antibody. Int Rev Cytol. 1980;63:59–95. doi: 10.1016/s0074-7696(08)61757-x. [DOI] [PubMed] [Google Scholar]
  4. Bryan J. Biochemical properties of microtubules. Fed Proc. 1974 Feb;33(2):152–157. [PubMed] [Google Scholar]
  5. Chi J. C., Fellini S. A., Holtzer H. Differences among myosins synthesized in non-myogenic cells, presumptive myoblasts, and myoblasts. Proc Natl Acad Sci U S A. 1975 Dec;72(12):4999–5003. doi: 10.1073/pnas.72.12.4999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Craig S. W., Pardo J. V. alpha-Actinin localization in the junctional complex of intestinal epithelial cells. J Cell Biol. 1979 Jan;80(1):203–210. doi: 10.1083/jcb.80.1.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Croop J., Holtzer H. Response of myogenic and fibrogenic cells to cytochalasin B and to colcemid. I. Light microscope observations. J Cell Biol. 1975 May;65(2):271–285. doi: 10.1083/jcb.65.2.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Croop J., Toyama Y., Dlugosz A. A., Holtzer H. Selective effects of phorbol 12-myristate 13-acetate on myofibrils and 10-nm filaments. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5273–5277. doi: 10.1073/pnas.77.9.5273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dienstman S. R., Holtzer H. Skeletal myogenesis. Control of proliferation in a normal cell lineage. Exp Cell Res. 1977 Jul;107(2):355–364. doi: 10.1016/0014-4827(77)90357-3. [DOI] [PubMed] [Google Scholar]
  10. Emerson C. P., Jr, Beckner S. K. Activation of myosin synthesis in fusing and mononucleated myoblasts. J Mol Biol. 1975 Apr 25;93(4):431–447. doi: 10.1016/0022-2836(75)90238-7. [DOI] [PubMed] [Google Scholar]
  11. Fellini S. A., Holtzer H. The localization of skeletal light meromyosin in cells of myogenic cultures. Differentiation. 1976 Aug 3;6(2):71–74. doi: 10.1111/j.1432-0436.1976.tb01471.x. [DOI] [PubMed] [Google Scholar]
  12. Franke W. W., Schmid E., Osborn M., Weber K. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5034–5038. doi: 10.1073/pnas.75.10.5034. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Goldstein M. A., Entman M. L. Microtubules in mammalian heart muscle. J Cell Biol. 1979 Jan;80(1):183–195. doi: 10.1083/jcb.80.1.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hayashi M., Ohnishi K., Hayashi K. Dense precipitate of brain tubulin with skeletal muscle myosin. J Biochem. 1980 May;87(5):1347–1355. doi: 10.1093/oxfordjournals.jbchem.a132874. [DOI] [PubMed] [Google Scholar]
  15. Holtzer H., Croop J., Dienstman S., Ishikawa H., Somlyo A. P. Effects of cytochaslasin B and colcemide on myogenic cultures. Proc Natl Acad Sci U S A. 1975 Feb;72(2):513–517. doi: 10.1073/pnas.72.2.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Holtzer H., Strahs K., Biehl J., Somlyo A. P., Ishikawa H. Thick and thin filaments in postmitotic, mononucleated myoblasts. Science. 1975 May 30;188(4191):943–945. doi: 10.1126/science.1138363. [DOI] [PubMed] [Google Scholar]
  17. Ishikawa H., Bischoff R., Holtzer H. Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol. 1969 Nov;43(2):312–328. [PMC free article] [PubMed] [Google Scholar]
  18. Ishikawa H., Bischoff R., Holtzer H. Mitosis and intermediate-sized filaments in developing skeletal muscle. J Cell Biol. 1968 Sep;38(3):538–555. doi: 10.1083/jcb.38.3.538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kligman D., Nameroff M. Analysis of the myogenic lineage in chick embryos. I. Studies on the terminal cell division. Exp Cell Res. 1980 Jan;125(1):201–210. doi: 10.1016/0014-4827(80)90204-9. [DOI] [PubMed] [Google Scholar]
  20. Moss P. S., Strohman R. C. Myosin synthesis by fusion-arrested chick embryo myoblasts in cell culture. Dev Biol. 1976 Feb;48(2):431–437. doi: 10.1016/0012-1606(76)90104-4. [DOI] [PubMed] [Google Scholar]
  21. Osborn M., Weber K. The display of microtubules in transformed cells. Cell. 1977 Nov;12(3):561–571. doi: 10.1016/0092-8674(77)90257-4. [DOI] [PubMed] [Google Scholar]
  22. Schiff P. B., Fant J., Horwitz S. B. Promotion of microtubule assembly in vitro by taxol. Nature. 1979 Feb 22;277(5698):665–667. doi: 10.1038/277665a0. [DOI] [PubMed] [Google Scholar]
  23. Schiff P. B., Horwitz S. B. Taxol stabilizes microtubules in mouse fibroblast cells. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1561–1565. doi: 10.1073/pnas.77.3.1561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shimo-Oka T., Hayashi M., Watanabe Y. Tubulin-myosin interaction. Some properties of binding between tubulin and myosin. Biochemistry. 1980 Oct 14;19(21):4921–4926. doi: 10.1021/bi00562a034. [DOI] [PubMed] [Google Scholar]
  25. Smith D. S., Järlfors U., Beránek R. The organization of synaptic axcplasm in the lamprey (petromyzon marinus) central nervous system. J Cell Biol. 1970 Aug;46(2):199–219. doi: 10.1083/jcb.46.2.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Spiegelman B. M., Lopata M. A., Kirschner M. W. Aggregation of microtubule initiation sites preceding neurite outgrowth in mouse neuroblastoma cells. Cell. 1979 Feb;16(2):253–263. doi: 10.1016/0092-8674(79)90003-5. [DOI] [PubMed] [Google Scholar]
  27. Spiegelman B. M., Lopata M. A., Kirschner M. W. Multiple sites for the initiation of microtubule assembly in mammalian cells. Cell. 1979 Feb;16(2):239–252. doi: 10.1016/0092-8674(79)90002-3. [DOI] [PubMed] [Google Scholar]
  28. Toyama Y., West C. M., Holtzer H. Differential response of myofibrils and 10-nm filaments to a cocarcinogen. Am J Anat. 1979 Sep;156(1):131–137. doi: 10.1002/aja.1001560114. [DOI] [PubMed] [Google Scholar]
  29. Van Winkle W. B., Schwartz A. Morphological and biochemical correlates of skeletal muscle contractility in the cat. I. Histochemical and electron microscopic studies. J Cell Physiol. 1978 Oct;97(1):99–119. doi: 10.1002/jcp.1040970110. [DOI] [PubMed] [Google Scholar]
  30. Warren R. H. The effect of colchicine on myogenesis in vivo in Rana pipiens and Rhodnius prolixus (Hemiptera). J Cell Biol. 1968 Dec;39(3):544–555. doi: 10.1083/jcb.39.3.544. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Yeoh G. C., Holtzer H. The effect of cell density, conditioned medium and cytosine arabinoside on myogenesis in primary and secondary cultures. Exp Cell Res. 1977 Jan;104(1):63–78. doi: 10.1016/0014-4827(77)90069-6. [DOI] [PubMed] [Google Scholar]

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