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. 1988 Jun;170(6):2796–2801. doi: 10.1128/jb.170.6.2796-2801.1988

High-efficiency transformation of bacterial cells by electroporation.

N M Calvin 1, P C Hanawalt 1
PMCID: PMC211205  PMID: 3286620

Abstract

We have developed a method for efficiently generating transient pores in the outer membranes of Escherichia coli K-12 derivatives by using a new type of electroporation apparatus. The pores are large enough and persist long enough to facilitate the equilibration of plasmid molecules between the intracellular and extracellular spaces. The method has been used to transform bacterial cells with an efficiency greater than 10(9) transformants per microgram of plasmid. It has also been used to extract intact plasmid from transformed cells with efficiencies comparable to those of the traditional alkaline lysis or CsCl equilibrium density gradient techniques. The technique is simple and rapid, allowing a transformation or the preparation of microgram quantities of plasmid to be accomplished in minutes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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