Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1982 Feb 1;92(2):343–349. doi: 10.1083/jcb.92.2.343

Ultrastructural localization of type V collagen in rat kidney

PMCID: PMC2112060  PMID: 7037794

Abstract

Antibodies specific for the alpha 1 (V) chain and native collagen molecules containing the alpha 1 (V) chain have been used in electron immunohistochemical studies of rat kidney to determine the ultrastructural distribution of this class of collagen molecules. In addition, antibodies against type I collagen and whole basement membrane were used as markers for interstitial collagen and authentic basement membranes. Our results indicate that type V collagen is present in the renal interstitium in different forms: in close apposition to interstitial collagen fibers; in the stromal aspect of vascular basement membranes; and as particulate material not bound to other structures. On the basis of these findings, we postulate a binding or connecting function for this collagen type.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bentz H., Bächinger H. P., Glanville R., Kühn K. Physical evidence for the assembly of A and B chains of human placental collagen in a single triple helix. Eur J Biochem. 1978 Dec;92(2):563–567. doi: 10.1111/j.1432-1033.1978.tb12778.x. [DOI] [PubMed] [Google Scholar]
  2. Biempica L., Morecki R., Wu C. H., Giambrone M. A., Rojkind M. Immunocytochemical localization of type B collagen: a component of basement membrane in human liver. Am J Pathol. 1980 Mar;98(3):591–602. [PMC free article] [PubMed] [Google Scholar]
  3. Bornstein P., Sage H. Structurally distinct collagen types. Annu Rev Biochem. 1980;49:957–1003. doi: 10.1146/annurev.bi.49.070180.004521. [DOI] [PubMed] [Google Scholar]
  4. Boselli J. M., Macarak E. J., Clark C. C., Brownell A. G., Martinez-Hernandez A. Fibronectin: its relationship to basement membranes. I. Light microscopic studies. Coll Relat Res. 1981 Sep;1(5):391–404. doi: 10.1016/s0174-173x(81)80024-6. [DOI] [PubMed] [Google Scholar]
  5. Burgeson R. E., El Adli F. A., Kaitila I. I., Hollister D. W. Fetal membrane collagens: identification of two new collagen alpha chains. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2579–2583. doi: 10.1073/pnas.73.8.2579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chung E., Rhodes K., Miller E. J. Isolation of three collagenous components of probable basement membrane origin from several tissues. Biochem Biophys Res Commun. 1976 Aug 23;71(4):1167–1174. doi: 10.1016/0006-291x(76)90776-2. [DOI] [PubMed] [Google Scholar]
  7. Gay R. E., Buckingham R. B., Prince R. K., Gay S., Rodnan G. P., Miller E. J. Collagen types synthesized in dermal fibroblast cultures from patients with early progressive systemic sclerosis. Arthritis Rheum. 1980 Feb;23(2):190–196. doi: 10.1002/art.1780230209. [DOI] [PubMed] [Google Scholar]
  8. Gay S., Martinez-Hernandez A., Rhodes R. K., Miller E. J. The collagenous exocytoskeleton of smooth muscle cells. Coll Relat Res. 1981 Jul;1(4):377–384. doi: 10.1016/s0174-173x(81)80014-3. [DOI] [PubMed] [Google Scholar]
  9. Gay S., Miller E. J. Characterization of lens capsule collagen: evidence for the presence of two unique chains in molecules derived from major basement membrane structures. Arch Biochem Biophys. 1979 Dec;198(2):370–378. doi: 10.1016/0003-9861(79)90509-5. [DOI] [PubMed] [Google Scholar]
  10. Gay S., Rhodes R. K., Gay R. E., Miller E. J. Collagen molecules comprised of alpha 1(V)-chains (B-chains): an apparent localization in the exocytoskeleton. Coll Relat Res. 1981;1(1):53–58. doi: 10.1016/s0174-173x(80)80007-0. [DOI] [PubMed] [Google Scholar]
  11. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  12. Hedman K. Intracellular localization of fibronectin using immunoperoxidase cytochemistry in light and electron microscopy. J Histochem Cytochem. 1980 Nov;28(11):1233–1241. doi: 10.1177/28.11.7000891. [DOI] [PubMed] [Google Scholar]
  13. Kawarai Y., Nakane P. K. Localization of tissue antigens on the ultrathin sections with peroxidase-labeled antibody method. J Histochem Cytochem. 1970 Mar;18(3):161–166. doi: 10.1177/18.3.161. [DOI] [PubMed] [Google Scholar]
  14. Kefalides N. A., Alper R., Clark C. C. Biochemistry and metabolism of basement membranes. Int Rev Cytol. 1979;61:167–228. doi: 10.1016/s0074-7696(08)61998-1. [DOI] [PubMed] [Google Scholar]
  15. Madri J. A., Dreyer B., Pitlick F. A., Furthmayr H. The collagenous components of the subendothelium. Correlation of structure and function. Lab Invest. 1980 Oct;43(4):303–315. [PubMed] [Google Scholar]
  16. Madri J. A., Furthmayr H. Collagen polymorphism in the lung. An immunochemical study of pulmonary fibrosis. Hum Pathol. 1980 Jul;11(4):353–366. doi: 10.1016/s0046-8177(80)80031-1. [DOI] [PubMed] [Google Scholar]
  17. Madri J. A., Furthmayr H. Isolation and tissue localization of type AB2 collagen from normal lung parenchyma. Am J Pathol. 1979 Feb;94(2):323–331. [PMC free article] [PubMed] [Google Scholar]
  18. Martinez-Hernandez A., Marsh C. A., Clark C. C., Macarak E. J., Brownell A. G. Fibronectin: its relationship to basement membranes. II. Ultrastructural studies in rat kidney. Coll Relat Res. 1981 Sep;1(5):405–418. doi: 10.1016/s0174-173x(81)80025-8. [DOI] [PubMed] [Google Scholar]
  19. Martinez-Hernandez A., Nakane P. K., Pierce G. B. Intracellular localization of basement membrane antigen in parietal yolk sac cells. Am J Pathol. 1974 Sep;76(3):549–560. [PMC free article] [PubMed] [Google Scholar]
  20. Nakane P. K. Application of peroxidase-labelled antibodies to the intracellular localization of hormones. Acta Endocrinol Suppl (Copenh) 1971;153:190–204. doi: 10.1530/acta.0.068s190. [DOI] [PubMed] [Google Scholar]
  21. Nakane P. K., Pierce G. B., Jr Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens. J Cell Biol. 1967 May;33(2):307–318. doi: 10.1083/jcb.33.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rhodes R. K., Miller E. J. Physicochemical characterization and molecular organization of the collagen A and B chains. Biochemistry. 1978 Aug 22;17(17):3442–3448. doi: 10.1021/bi00610a003. [DOI] [PubMed] [Google Scholar]
  23. Roll F. J., Madri J. A., Albert J., Furthmayr H. Codistribution of collagen types IV and AB2 in basement membranes and mesangium of the kidney. an immunoferritin study of ultrathin frozen sections. J Cell Biol. 1980 Jun;85(3):597–616. doi: 10.1083/jcb.85.3.597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sage H., Bornstein P. Characterization of a novel collagen chain in human placenta and its relation to AB collagen. Biochemistry. 1979 Aug 21;18(17):3815–3822. doi: 10.1021/bi00584a027. [DOI] [PubMed] [Google Scholar]
  25. Sherman M. I., Gay R., Gay S., Miller E. J. Association of collagen with preimplantation and peri-implantation mouse embryos. Dev Biol. 1980 Feb;74(2):470–478. doi: 10.1016/0012-1606(80)90446-7. [DOI] [PubMed] [Google Scholar]
  26. Welsh C., Gay S., Rhodes R. K., Pfister R., Miller E. J. Collagen heterogeneity in normal rabbit cornea. I. Isolation and biochemical characterization of the genetically-distinct collagens. Biochim Biophys Acta. 1980 Sep 23;625(1):78–88. doi: 10.1016/0005-2795(80)90110-5. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES