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. 1982 Jul 1;94(1):193–200. doi: 10.1083/jcb.94.1.193

Reexpression of blood group ABH antigens on the surface of human thyroid cells in culture

PMCID: PMC2112191  PMID: 6749867

Abstract

Using indirect immunofluorescence (IFL) on viable human thyroid cultures, it has been shown that, although adult follicular cells do not express blood group ABH antigens in vivo, they invariably reexpress the corresponding antigens on the cell surface when cultured in monolayers, even for very short periods. The absence of blood group antigens on noncultured thyroid cells was confirmed by negative IFL on cell suspensions obtained after enzymatic digestion of the glands, whereas these antigens were readily demonstrable on cell suspensions obtained by trypsinization of established monolayers. The quantitative expression of ABH antigens on individual thyroid cells was variable and the cell-surface IFL pattern due to binding of blood group isoantibodies was different from that given by organ-specific thyroid autoantibodies on viable cultures. Reexpression of blood group antigens by cultured thyroid cells could not be related to the secretor status of the donors, the presence of a particular source of serum in the culture medium or cell division in vitro. After 2-3 wk in culture, thyroid cells became morphologically dedifferentiated and no longer displayed blood group antigens, though they still expressed cell- surface beta 2-microglobulin. Fibroblasts present in the primary thyroid cultures were invariably negative for ABH antigens. These results demonstrate that the surface antigenic repertoire of cultured human cells is not necessarily identical to that present on the same cells in vivo. Furthermore, the possibility that blood group natural isoantibodies bind to the cell surface must be taken into account in experiments in which cultured thyroid cells are exposed to human sera.

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Selected References

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  1. BHENDE Y. M., DESHPANDE C. K., BHATIA H. M., SANGER R., RACE R. R., MORGAN W. T. J., WATKINS W. M. A "new" blood group character related to the ABO system. Lancet. 1952 May 3;1(6714):903–904. [PubMed] [Google Scholar]
  2. Bidey S. P., Marsden P., Anderson J., McKerron C. G., Berry H. In-vitro studies of normal human thyroid cells: responses to thyrotrophin and dibutyryl cyclic AMP. J Endocrinol. 1977 Jan;72(1):87–96. doi: 10.1677/joe.0.0720087. [DOI] [PubMed] [Google Scholar]
  3. CHESSIN L. N., BRAMSON S., KUHNS W. J., HIRSCHHORN K. STUDIES ON THE A, B, O(H) BLOOD GROUPS ON HUMAN CELLS IN CULTURE. Blood. 1965 Jun;25:944–953. [PubMed] [Google Scholar]
  4. COOMBS R. R., BEDFORD D., ROUILLARD L. M. A and B blood-group antigens on human epidermal cells demonstrated by mized agglutination. Lancet. 1956 Apr 21;270(6921):461–463. doi: 10.1016/s0140-6736(56)90528-1. [DOI] [PubMed] [Google Scholar]
  5. Dawson A., Franks D. Factors affecting the expression of blood group antigen A in cultured cells. Exp Cell Res. 1967 Aug;47(1):377–385. doi: 10.1016/0014-4827(67)90240-6. [DOI] [PubMed] [Google Scholar]
  6. FORBES I. J., ROITT I. M., DONIACH D., SOLOMON I. L. The thyroid cytotoxic autoantibody. J Clin Invest. 1962 May;41:996–1006. doi: 10.1172/JCI104579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FRASER G. R., MORGANS M. E., TROTTER W. R. The syndrome of sporadic goitre and congenital deafness. Q J Med. 1960 Apr;29:279–295. [PubMed] [Google Scholar]
  8. Franks D., Dawson A. Variation in the expression of blood group antigen A in clonal cultures of rabbit cells. Exp Cell Res. 1966 Jun;42(3):543–561. doi: 10.1016/0014-4827(66)90268-0. [DOI] [PubMed] [Google Scholar]
  9. GLYNN L. E., HOLBOROW E. J., JOHNSON G. D. The distribution of blood-group substances in human gastric and duodenal mucosa. Lancet. 1957 Nov 30;273(7005):1083–1088. doi: 10.1016/s0140-6736(57)90115-0. [DOI] [PubMed] [Google Scholar]
  10. GLYNN L. E., HOLBOROW E. J. [Distribution of blood-group substances in human tissues]. Br Med Bull. 1959 May;15(2):150–153. doi: 10.1093/oxfordjournals.bmb.a069740. [DOI] [PubMed] [Google Scholar]
  11. HODGES R. E., EVANS T. C., BRADBURY J. T., KEETTEL W. C. The accumulation of radioactive iodine by human fetal thyroids. J Clin Endocrinol Metab. 1955 Jun;15(6):661–667. doi: 10.1210/jcem-15-6-661. [DOI] [PubMed] [Google Scholar]
  12. HOLBOROW E. J., BROWN P. C., GLYNN L. E., HAWES M. D., GRESHAM G. A., O'BRIEN T. F., COOMBS R. R. The distribution of the blood group. A antigen in human tissues. Br J Exp Pathol. 1960 Aug;41:430–437. [PMC free article] [PubMed] [Google Scholar]
  13. Hall R., Amos J., Ormston B. J. Radioimmunoassay of human serum thyrotrophin. Br Med J. 1971 Mar 13;1(5749):582–585. doi: 10.1136/bmj.1.5749.582. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jonsson J., Fagraeus A., Biberfeld G. The mixed haemadsorption test as an aid to the diagnosis of thyroid autoimmune disease. Clin Exp Immunol. 1968 May;3(4):287–304. [PMC free article] [PubMed] [Google Scholar]
  15. Khoury E. L., Hammond L., Bottazzo G. F., Doniach D. Presence of the organ-specific 'microsomal' autoantigen on the surface of human thyroid cells in culture: its involvement in complement-mediated cytotoxicity. Clin Exp Immunol. 1981 Aug;45(2):316–328. [PMC free article] [PubMed] [Google Scholar]
  16. Kite J. H., Jr, Rose N. R., Kano K., Witebsky E. Cytotoxicity of human thyroid autoantibodies. Ann N Y Acad Sci. 1965 Jun 30;124(2):626–643. doi: 10.1111/j.1749-6632.1965.tb18991.x. [DOI] [PubMed] [Google Scholar]
  17. Kuhns W. J., Bramson S. Variable behaviour of blood group H on HeLa cell populations synchronized with thymidine. Nature. 1968 Aug 31;219(5157):938–939. doi: 10.1038/219938a0. [DOI] [PubMed] [Google Scholar]
  18. Lee S. K., Singh J., Taylor R. B. Subclasses of T cells with different sensitivities to cytotoxic antibody in the presence of anesthetics. Eur J Immunol. 1975 Apr;5(4):259–262. doi: 10.1002/eji.1830050408. [DOI] [PubMed] [Google Scholar]
  19. Nicol A. G., Beck J. S. Persistence of an organ-specific antigen in organ and tissue cultures of hyperplastic human thyroid gland. Nature. 1966 Jun 18;210(5042):1227–1229. doi: 10.1038/2101227a0. [DOI] [PubMed] [Google Scholar]
  20. PULVERTAFT R. J., DONIACH D., ROITT I. M., HUDSON R. V. Cytotoxic effects of Hashimoto serum on human thyroid cells in tissue culture. Lancet. 1959 Aug 29;2(7096):214–216. doi: 10.1016/s0140-6736(59)90161-8. [DOI] [PubMed] [Google Scholar]
  21. Pekonen F., Weintraub B. D. Thyrotropin binding to cultured lymphocytes and thyroid cells. Endocrinology. 1978 Nov;103(5):1668–1677. doi: 10.1210/endo-103-5-1668. [DOI] [PubMed] [Google Scholar]
  22. SZULMAN A. E. THE HISTOLOGICAL DISTRIBUTION OF THE BLOOD GROUP SUBSTANCES IN MAN AS DISCLOSED BY IMMUNOFLUORESCENCE. III. THE A, B, AND H ANTIGENS IN EMBRYOS AND FETUSES FROM 18 MM IN LENGTH. J Exp Med. 1964 Apr 1;119:503–516. doi: 10.1084/jem.119.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SZULMAN A. E. The histological distribution of blood group substances A and B in man. J Exp Med. 1960 Jun 1;111:785–800. doi: 10.1084/jem.111.6.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Thomas D. B. Cyclic expression of blood group determinants in murine cells and their relationship to growth control. Nature. 1971 Oct 1;233(5318):317–321. doi: 10.1038/233317a0. [DOI] [PubMed] [Google Scholar]
  25. Toccafondi R. S., Aterini S., Medici M. A., Rotella C. M., Tanini A., Zonefrati R. Thyroid-stimulating antibody (TSab) detected in sera of Graves' patients using human thyroid cell cultures. Clin Exp Immunol. 1980 Jun;40(3):532–539. [PMC free article] [PubMed] [Google Scholar]
  26. Toh B. H., Diggle T. A., Koh S. H. Ii blood group antigens on fibroblast cell surfaces. Clin Immunol Immunopathol. 1979 Feb;12(2):177–182. doi: 10.1016/0090-1229(79)90006-0. [DOI] [PubMed] [Google Scholar]
  27. YUNIS E. J., YUNIS J. J., BRAND K. G. DEMONSTRATION OF ISOANTIGENIC RECEPTORS ON HUMAN EPIDERMAL CELLS AND ESTABLISHED CELL LINES. Blood. 1965 Jan;25:96–104. [PubMed] [Google Scholar]
  28. YUNIS E., YUNIS J. J. CELL ANTIGENS AND CELL SPECIALIZATION. III. ON THE H ANTIGEN RECEPTORS OF HUMAN EPIDERMAL CELLS. Blood. 1963 Dec;22:750–756. [PubMed] [Google Scholar]
  29. Yamada K. M., Olden K. Fibronectins--adhesive glycoproteins of cell surface and blood. Nature. 1978 Sep 21;275(5677):179–184. doi: 10.1038/275179a0. [DOI] [PubMed] [Google Scholar]

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