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. 1982 Jul 1;94(1):108–114. doi: 10.1083/jcb.94.1.108

Properties of extracellular adhesion-mediating particles in myoblast clone and its adhesion-deficient variant

PMCID: PMC2112192  PMID: 7119009

Abstract

Both the skeletal muscle myoblast cell line L6 and an adhesion- deficient variant of L6 released glycoprotein complexes, termed adherons, into their culture medium. The adherons from the variant, M3A, differed from those of L6 in a number of properties. M3A adherons were much less effective in promoting the cell-substratum and cell-cell adhesion of myoblasts than L6 particles. The adherons from the two cell lines also differed in their relative sedimentation velocities in sucrose gradients and had different chemical compositions. The M3A particle lacked chondroitin and contained relatively less collagen and fibronectin than the L6 adheron. Both L6 and M3A particles adhered to plastic surfaces and cells equally well in the absence of calcium ions. Neither cell-cell adhesion nor particle aggregation occurred in calcium- free medium. However, in the presence of calcium, the L6 adherons aggregated completely and M3A particles aggregated poorly. These data suggest that at least two sets of interactions are required for adheron- mediated adhesion: a calcium-independent binding of the adheron to the cell, and a calcium-dependent interaction between particles that is directly responsible for adhesion. The M3A variant is blocked at the calcium-dependent step, resulting in an adhesion deficiency.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. W., Baum P. R., Gesteland R. F. Processing of adenovirus 2-induced proteins. J Virol. 1973 Aug;12(2):241–252. doi: 10.1128/jvi.12.2.241-252.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Collins F. Neurite outgrowth induced by the substrate associated material from nonneuronal cells. Dev Biol. 1980 Sep;79(1):247–252. doi: 10.1016/0012-1606(80)90089-5. [DOI] [PubMed] [Google Scholar]
  3. Henkart P., Humphreys S., Humphreys T. Characterization of sponge aggregation factor. A unique proteoglycan complex. Biochemistry. 1973 Jul 31;12(16):3045–3050. doi: 10.1021/bi00740a016. [DOI] [PubMed] [Google Scholar]
  4. Hynes R. O., Destree A. T., Perkins M. E., Wagner D. D. Cell surface fibronectin and oncogenic transformation. J Supramol Struct. 1979;11(1):95–104. doi: 10.1002/jss.400110110. [DOI] [PubMed] [Google Scholar]
  5. Jumblatt J. E., Schlup V., Burger M. M. Cell-cell recognition: specific binding of Microciona sponge aggregation factor to homotypic cells and the role of calcium ions. Biochemistry. 1980 Mar 4;19(5):1038–1042. doi: 10.1021/bi00546a032. [DOI] [PubMed] [Google Scholar]
  6. Klebe R. J., Rosenberger P. G., Naylor S. L., Burns R. L., Novak R., Kleinman H. Cell attachment to collagen. Isolation of a cell attachment mutant. Exp Cell Res. 1977 Jan;104(1):119–125. doi: 10.1016/0014-4827(77)90074-x. [DOI] [PubMed] [Google Scholar]
  7. Knudsen K. A., Horwitz A. F. Differential inhibition of myoblast fusion. Dev Biol. 1978 Oct;66(2):294–307. doi: 10.1016/0012-1606(78)90239-7. [DOI] [PubMed] [Google Scholar]
  8. McClay D. R., Moscona A. A. Purification of the specific cell-aggregating factor from embryonic neural retina cells. Exp Cell Res. 1974 Aug;87(2):438–443. doi: 10.1016/0014-4827(74)90514-x. [DOI] [PubMed] [Google Scholar]
  9. Obrink B., Kuhlenschmidt M. S., Roseman S. Adhesive specificity of juvenile rat and chicken liver cells and membranes. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1077–1081. doi: 10.1073/pnas.74.3.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saito H., Yamagata T., Suzuki S. Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates. J Biol Chem. 1968 Apr 10;243(7):1536–1542. [PubMed] [Google Scholar]
  11. Sanes J. R., Marshall L. M., McMahan U. J. Reinnervation of muscle fiber basal lamina after removal of myofibers. Differentiation of regenerating axons at original synaptic sites. J Cell Biol. 1978 Jul;78(1):176–198. doi: 10.1083/jcb.78.1.176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schubert D., LaCorbiere M. A role of secreted glycosaminoglycans in cell-substratum adhesion. J Biol Chem. 1980 Dec 10;255(23):11564–11569. [PubMed] [Google Scholar]
  13. Schubert D., LaCorbiere M. Altered collagen and glycosaminoglycan secretion by a skeletal muscle myoblast variant. J Biol Chem. 1980 Dec 10;255(23):11557–11563. [PubMed] [Google Scholar]
  14. Schubert D., LaCorbiere M. Role of a 16S glycoprotein complex in cellular adhesion. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4137–4141. doi: 10.1073/pnas.77.7.4137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schubert D., LaCorbiere M. The specificity of extracellular glycoprotein complexes in mediating cellular adhesion. J Neurosci. 1982 Jan;2(1):82–89. doi: 10.1523/JNEUROSCI.02-01-00082.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Starger J. M., Brown W. E., Goldman A. E., Goldman R. D. Biochemical and immunological analysis of rapidly purified 10-nm filaments from baby hamster kidney (BHK-21) cells. J Cell Biol. 1978 Jul;78(1):93–109. doi: 10.1083/jcb.78.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Takeichi M., Atsumi T., Yoshida C., Uno K., Okada T. S. Selective adhesion of embryonal carcinoma cells and differentiated cells by Ca2+-dependent sites. Dev Biol. 1981 Oct 30;87(2):340–350. doi: 10.1016/0012-1606(81)90157-3. [DOI] [PubMed] [Google Scholar]
  18. Thiery J. P., Brackenbury R., Rutishauser U., Edelman G. M. Adhesion among neural cells of the chick embryo. II. Purification and characterization of a cell adhesion molecule from neural retina. J Biol Chem. 1977 Oct 10;252(19):6841–6845. [PubMed] [Google Scholar]
  19. Thomas W. A., Edelman B. A., Lobel S. M., Breitbart A. S., Steinberg M. S. Two chick embryonic adhesion systems: molecular vs tissue specificity. J Supramol Struct Cell Biochem. 1981;16(1):15–27. doi: 10.1002/jsscb.1981.380160103. [DOI] [PubMed] [Google Scholar]
  20. Yaffe D. Retention of differentiation potentialities during prolonged cultivation of myogenic cells. Proc Natl Acad Sci U S A. 1968 Oct;61(2):477–483. doi: 10.1073/pnas.61.2.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yamada K. M., Olden K. Fibronectins--adhesive glycoproteins of cell surface and blood. Nature. 1978 Sep 21;275(5677):179–184. doi: 10.1038/275179a0. [DOI] [PubMed] [Google Scholar]

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