Abstract
The regularly repeating periodic nucleosome organization is clearly resolved in the chromatin of the isolated salivary chromosomes of Drosophila melanogaster. A new microsurgical procedure of isolation in buffer A of Hewish and Burgoyne (1973, Biochem. Biophys. Res. Commun., 52:504-510) yielded native Drosophila salivary chromosomes. These chromosomes were then swollen and spread by a modified Miller procedure, stained or shadowed, and examined in the electron microscope. Individual nucleoprotein fibers were resolved with regularly repeated nucleosomes of approximately 10 nm diameter. Micrococcal nuclease digestion of isolated salivary nuclei gave a family of DNA fragments characteristic of nucleosomes for total chromatin, 5S gene, and simple satellite (rho = 1.688 g/cm3) sequences.
Full Text
The Full Text of this article is available as a PDF (1.9 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Artavanis-Tsakonas S., Schedl P., Tschudi C., Pirrotta V., Steward R., Gehring W. J. The 5S genes of Drosophila melanogaster. Cell. 1977 Dec;12(4):1057–1067. doi: 10.1016/0092-8674(77)90169-6. [DOI] [PubMed] [Google Scholar]
- Griffith J. D. Chromatin structure: deduced from a minichromosome. Science. 1975 Mar 28;187(4182):1202–1203. doi: 10.1126/science.187.4182.1202. [DOI] [PubMed] [Google Scholar]
- Hewish D. R., Burgoyne L. A. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973 May 15;52(2):504–510. doi: 10.1016/0006-291x(73)90740-7. [DOI] [PubMed] [Google Scholar]
- Hill R. J., Watt F. "Native" salivary chromosomes of Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):859–865. doi: 10.1101/sqb.1978.042.01.087. [DOI] [PubMed] [Google Scholar]
- Hsieh T., Brutlag D. Sequence and sequence variation within the 1.688 g/cm3 satellite DNA of Drosophila melanogaster. J Mol Biol. 1979 Dec 5;135(2):465–481. doi: 10.1016/0022-2836(79)90447-9. [DOI] [PubMed] [Google Scholar]
- McMaster-Kaye R., Kaye J. S. Organization of chromatin during spermiogenesis: beaded fibers, partly beaded fibers, and loss of nucleosomal structure. Chromosoma. 1980;77(1):41–56. doi: 10.1007/BF00292040. [DOI] [PubMed] [Google Scholar]
- Miller O. L., Jr, Beatty B. R. Visualization of nucleolar genes. Science. 1969 May 23;164(3882):955–957. doi: 10.1126/science.164.3882.955. [DOI] [PubMed] [Google Scholar]
- Olins A. L., Olins D. E., Zentgraf H., Franke W. W. Visualization of nucleosomes in thin sections by stereo electron microscopy. J Cell Biol. 1980 Dec;87(3 Pt 1):833–836. doi: 10.1083/jcb.87.3.833. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rattner J. B., Branch A., Hamkalo B. A. Electron microscopy of whole mount metaphase chromosomes. Chromosoma. 1975 Nov 11;52(4):329–338. doi: 10.1007/BF00364017. [DOI] [PubMed] [Google Scholar]
- Woodcock C. L., Safer J. P., Stanchfield J. E. Structural repeating units in chromatin. I. Evidence for their general occurrence. Exp Cell Res. 1976 Jan;97:101–110. doi: 10.1016/0014-4827(76)90659-5. [DOI] [PubMed] [Google Scholar]
- Wu C., Bingham P. M., Livak K. J., Holmgren R., Elgin S. C. The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence. Cell. 1979 Apr;16(4):797–806. doi: 10.1016/0092-8674(79)90095-3. [DOI] [PubMed] [Google Scholar]