Abstract
A 75,000-dalton protein has been purified approximately 1,000-fold from rat liver, based on its capacity to organize poly(A) in a 27-residue repeating structure. This protein may be identified with the major polypeptide component of cytoplasmic poly(A)-ribonucleoprotein (RNP) previously described. The poly(A)-organizing activity of the protein is detected only in cytoplasmic fractions. Upon nuclease digestion of the 75,000-dalton protein-poly(A) complex, monomers, and higher multimers of RNP subunits can be resolved in a sucrose gradient. The sedimentation rate of the monomer is compatible with a composition of one 75,000-dalton protein molecule and one 27-residue segment of poly(A).
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Selected References
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- Baer B. W., Kornberg R. D. Repeating structure of cytoplasmic poly(A)-ribonucleoprotein. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1890–1892. doi: 10.1073/pnas.77.4.1890. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barrieux A., Ingraham H. A., Nystul S., Rosenfeld M. G. Characterization of the association of specific proteins with messenger ribonucleic acid. Biochemistry. 1976 Aug 10;15(16):3523–3528. doi: 10.1021/bi00661a020. [DOI] [PubMed] [Google Scholar]
- Finch J. T., Noll M., Kornberg R. D. Electron microscopy of defined lengths of chromatin. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3320–3322. doi: 10.1073/pnas.72.9.3320. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Firtel R. A., Pederson T. Ribonucleoprotein particles containing heterogeneous nuclear RNA in the cellular slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1975 Jan;72(1):301–305. doi: 10.1073/pnas.72.1.301. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fukami H., Itano H. A. In vitro translation of globin: effect of proteins purified by affinity chromatography on polyadenylate-Sepharose. Biochemistry. 1976 Aug 10;15(16):3529–3535. doi: 10.1021/bi00661a021. [DOI] [PubMed] [Google Scholar]
- Kish V. M., Pederson T. Poly (A)-rich ribonucleoprotein complexes from HeLa cell messenger RNA. J Biol Chem. 1976 Oct 10;251(19):5888–5894. [PubMed] [Google Scholar]
- Kish V. M., Pederson T. Ribonucleoprotein organization of polyadenylate sequences in HeLa cell heterogeneous nuclear RNA. J Mol Biol. 1975 Jun 25;95(2):227–238. doi: 10.1016/0022-2836(75)90392-7. [DOI] [PubMed] [Google Scholar]
- Kwan S. W., Brawerman G. A particle associated with the polyadenylate segment in mammalian messenger RNA. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3247–3250. doi: 10.1073/pnas.69.11.3247. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Laskey R. A., Honda B. M., Mills A. D., Finch J. T. Nucleosomes are assembled by an acidic protein which binds histones and transfers them to DNA. Nature. 1978 Oct 5;275(5679):416–420. doi: 10.1038/275416a0. [DOI] [PubMed] [Google Scholar]
- Lindberg U., Sundquist B. Isolation of messenger ribonucleoproteins from mammalian cells. J Mol Biol. 1974 Jun 25;86(2):451–468. doi: 10.1016/0022-2836(74)90030-8. [DOI] [PubMed] [Google Scholar]
- Maniatis T., Jeffrey A., van deSande H. Chain length determination of small double- and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry. 1975 Aug 26;14(17):3787–3794. doi: 10.1021/bi00688a010. [DOI] [PubMed] [Google Scholar]
- Morel C., Gander E. S., Herzberg M., Dubochet J., Scherrer K. The duck-globin messenger-ribonucleoprotein complex. Resistance to high ionic strength, particle gel electrophoresis, composition and visualisation by dark-field electron microscopy. Eur J Biochem. 1973 Jul 16;36(2):455–464. doi: 10.1111/j.1432-1033.1973.tb02930.x. [DOI] [PubMed] [Google Scholar]
- Quinlan T. J., Billings P. B., Martin T. E. Nuclear ribonucleoprotein complexes containing polyadenylate from mouse ascites cells. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2632–2636. doi: 10.1073/pnas.71.7.2632. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartz H., Darnell J. E. The association of protein with the polyadenylic acid of HeLa cell messenger RNA: evidence for a "transport" role of a 75,000 molecular weight polypeptide. J Mol Biol. 1976 Jul 15;104(4):833–851. doi: 10.1016/0022-2836(76)90185-6. [DOI] [PubMed] [Google Scholar]
- Schweiger A., Mazur G. Rat liver soluble nuclear and cytoplasmic proteins with high affinity to polynucleotides. FEBS Lett. 1975 Dec 1;60(1):114–118. doi: 10.1016/0014-5793(75)80430-3. [DOI] [PubMed] [Google Scholar]