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. 1983 Jun 1;96(6):1532–1537. doi: 10.1083/jcb.96.6.1532

Teratocarcinoma stem cell adhesion: the role of divalent cations and a cell surface lectin

PMCID: PMC2112446  PMID: 6406515

Abstract

We describe two additive systems of intercellular adhesion in teratocarcinoma stem cells (Nulli cell line). One component is divalent cation-dependent (Ca++ or Mg++) and the other involves a cell surface fucan/mannan-specific lectin, previously identified on stem cells by an erythrocyte rosetting assay. The existence of these two systems is inferred from the observation that reaggregation of stem cells was partially inhibited by the removal of divalent cations or by the presence of lectin inhibitors such as fucoidan, but reaggregation was completely blocked when the two conditions were combined. Our results are related to recent work describing a calcium-dependent system of intercellular adhesion in teratocarcinoma stem cells.

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Selected References

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  1. Ashwell G., Harford J. Carbohydrate-specific receptors of the liver. Annu Rev Biochem. 1982;51:531–554. doi: 10.1146/annurev.bi.51.070182.002531. [DOI] [PubMed] [Google Scholar]
  2. Bolwell G. P., Callow J. A., Evans L. V. Fertilization in brown algae. III. Preliminary characterization of putative gamete receptors from eggs and sperm of Fucus serratus. J Cell Sci. 1980 Jun;43:209–224. doi: 10.1242/jcs.43.1.209. [DOI] [PubMed] [Google Scholar]
  3. Brackenbury R., Rutishauser U., Edelman G. M. Distinct calcium-independent and calcium-dependent adhesion systems of chicken embryo cells. Proc Natl Acad Sci U S A. 1981 Jan;78(1):387–391. doi: 10.1073/pnas.78.1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Frazier W., Glaser L. Surface components and cell recognition. Annu Rev Biochem. 1979;48:491–523. doi: 10.1146/annurev.bi.48.070179.002423. [DOI] [PubMed] [Google Scholar]
  5. Glabe C. G., Grabel L. B., Vacquier V. D., Rosen S. D. Carbohydrate specificity of sea urchin sperm bindin: a cell surface lectin mediating sperm-egg adhesion. J Cell Biol. 1982 Jul;94(1):123–128. doi: 10.1083/jcb.94.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gooi H. C., Feizi T., Kapadia A., Knowles B. B., Solter D., Evans M. J. Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains. Nature. 1981 Jul 9;292(5819):156–158. doi: 10.1038/292156a0. [DOI] [PubMed] [Google Scholar]
  7. Grabel L. B., Glabe C. G., Singer M. S., Martin G. R., Rosen S. D. A fucan specific lectin on teratocarcinoma stem cells. Biochem Biophys Res Commun. 1981 Oct 30;102(4):1165–1171. doi: 10.1016/s0006-291x(81)80134-9. [DOI] [PubMed] [Google Scholar]
  8. Grabel L. B., Rosen S. D., Martin G. R. Teratocarcinoma stem cells have a cell surface carbohydrate-binding component implicated in cell-cell adhesion. Cell. 1979 Jul;17(3):477–484. doi: 10.1016/0092-8674(79)90255-1. [DOI] [PubMed] [Google Scholar]
  9. Grunwald G. B., Geller R. L., Lilien J. Enzymatic dissection of embryonic cell adhesive mechanisms. J Cell Biol. 1980 Jun;85(3):766–776. doi: 10.1083/jcb.85.3.766. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hughes R. C., Sharon A. Carbohydrates recognised. Nature. 1978 Aug 17;274(5672):637–638. doi: 10.1038/274637a0. [DOI] [PubMed] [Google Scholar]
  11. Hyafil F., Morello D., Babinet C., Jacob F. A cell surface glycoprotein involved in the compaction of embryonal carcinoma cells and cleavage stage embryos. Cell. 1980 Oct;21(3):927–934. doi: 10.1016/0092-8674(80)90456-0. [DOI] [PubMed] [Google Scholar]
  12. Magnani J. L., Thomas W. A., Steinberg M. S. Two distinct adhesion mechanisms in embryonic neural retina cells. I. A kinetic analysis. Dev Biol. 1981 Jan 15;81(1):96–105. doi: 10.1016/0012-1606(81)90351-1. [DOI] [PubMed] [Google Scholar]
  13. Martin G. R., Evans M. J. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1441–1445. doi: 10.1073/pnas.72.4.1441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Muramatsu T., Gachelin G., Nicolas J. F., Condamine H., Jakob H., Jacob F. Carbohydrate structure and cell differentitation: unique properties of fucosyl-glycopeptides isolated from embryonal carcinoma cells. Proc Natl Acad Sci U S A. 1978 May;75(5):2315–2319. doi: 10.1073/pnas.75.5.2315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ogou S., Okada T. S., Takeichi M. Cleavage stage mouse embryos share a common cell adhesion system with teratocarcinoma cells. Dev Biol. 1982 Aug;92(2):521–528. doi: 10.1016/0012-1606(82)90196-8. [DOI] [PubMed] [Google Scholar]
  16. Rosen S. D., Chang C. M., Barondes S. H. Intercellular adhesion in the cellular slime mold Polysphondylium pallidum inhibited by interaction of asialofetuin or specific univalent antibody with endogenous cell surface lectin. Dev Biol. 1977 Dec;61(2):202–213. doi: 10.1016/0012-1606(77)90292-5. [DOI] [PubMed] [Google Scholar]
  17. Solter D., Knowles B. B. Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565–5569. doi: 10.1073/pnas.75.11.5565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stoolman L. M., Rosen S. D. Possible role for cell-surface carbohydrate-binding molecules in lymphocyte recirculation. J Cell Biol. 1983 Mar;96(3):722–729. doi: 10.1083/jcb.96.3.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Takeichi M., Atsumi T., Yoshida C., Uno K., Okada T. S. Selective adhesion of embryonal carcinoma cells and differentiated cells by Ca2+-dependent sites. Dev Biol. 1981 Oct 30;87(2):340–350. doi: 10.1016/0012-1606(81)90157-3. [DOI] [PubMed] [Google Scholar]
  20. Takeichi M. Functional correlation between cell adhesive properties and some cell surface proteins. J Cell Biol. 1977 Nov;75(2 Pt 1):464–474. doi: 10.1083/jcb.75.2.464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yoshida C., Takeichi M. Teratocarcinoma cell adhesion: identification of a cell-surface protein involved in calcium-dependent cell aggregation. Cell. 1982 Feb;28(2):217–224. doi: 10.1016/0092-8674(82)90339-7. [DOI] [PubMed] [Google Scholar]

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