Abstract
3T3 plasma membranes were added to subconfluent cultures of SV3T3 cells in the presence of fusogens. If this protocol results in the introduction into the SV3T3 cell membrane of 3T3 plasma membrane components responsible for density-dependent inhibition of growth, then the SV3T3 cell cultures would be expected to show decreased rates of DNA synthesis as they approach confluence. Results of these experiments indicate that rates of DNA synthesis in SV3T3 cultures so treated were as much as 63% less than in untreated controls. This effect could not be attributed to the fusogens or to the 3T3 plasma membranes alone. This growth-inhibitory effect is specific for 3T3 membranes and is not observed when SV3T3 plasma membranes are fused with SV3T3 cell cultures. These data support the hypothesis that one aspect of the loss of density-dependent inhibition of growth in SV3T3 cells is a deletion or alteration in plasma membrane components and, further, that density- dependent inhibition of growth can be in part restored to SV3T3 cell cultures by fusing the cells with 3T3 plasma membranes.
Full Text
The Full Text of this article is available as a PDF (843.9 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Das M., Fox C. F. Molecular mechanism of mitogen action: processing of receptor induced by epidermal growth factor. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2644–2648. doi: 10.1073/pnas.75.6.2644. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dulbecco R., Stoker M. G. Conditions determining initiation of DNA synthesis in 3T3 cells. Proc Natl Acad Sci U S A. 1970 May;66(1):204–210. doi: 10.1073/pnas.66.1.204. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dulbecco R. Topoinhibition and serum requirement of transformed and untransformed cells. Nature. 1970 Aug 22;227(5260):802–806. doi: 10.1038/227802a0. [DOI] [PubMed] [Google Scholar]
- EAGLE H. METABOLIC CONTROLS IN CULTURED MAMMALIAN CELLS. Science. 1965 Apr 2;148(3666):42–51. doi: 10.1126/science.148.3666.42. [DOI] [PubMed] [Google Scholar]
- Gahmberg C. G., Hakomori S. I. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem. 1973 Jun 25;248(12):4311–4317. [PubMed] [Google Scholar]
- Goldberg A. L., Dice J. F. Intracellular protein degradation in mammalian and bacterial cells. Annu Rev Biochem. 1974;43(0):835–869. doi: 10.1146/annurev.bi.43.070174.004155. [DOI] [PubMed] [Google Scholar]
- Peterson S. W., Lerch V., Moynahan M. E., Carson M. P., Vale R. Partial characterization of a growth-inhibiting protein in 3T3 cell plasma membranes. Exp Cell Res. 1982 Dec;142(2):447–451. doi: 10.1016/0014-4827(82)90386-x. [DOI] [PubMed] [Google Scholar]
- Poste G., Nicolson G. L. Arrest and metastasis of blood-borne tumor cells are modified by fusion of plasma membrane vesicles from highly metastatic cells. Proc Natl Acad Sci U S A. 1980 Jan;77(1):399–403. doi: 10.1073/pnas.77.1.399. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raben D., Lieberman M. A., Glaser L. Growth inhibitory protein(s) in the 3T3 cell plasma membrane. Partial purification and dissociation of growth inhibitory events from inhibition of amino acid transport. J Cell Physiol. 1981 Jul;108(1):35–45. doi: 10.1002/jcp.1041080106. [DOI] [PubMed] [Google Scholar]
- TODARO G. J., GREEN H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J Cell Biol. 1963 May;17:299–313. doi: 10.1083/jcb.17.2.299. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whittenberger B., Glaser L. Inhibition of DNA synthesis in cultures of 3T3 cells by isolated surface membranes. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2251–2255. doi: 10.1073/pnas.74.6.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whittenberger B., Raben D., Lieberman M. A., Glaser L. Inhibition of growth of 3T3 cells by extract of surface membranes. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5457–5461. doi: 10.1073/pnas.75.11.5457. [DOI] [PMC free article] [PubMed] [Google Scholar]