Abstract
Halobacteroides acetoethylicus grew in media with 6 to 20% NaCl and displayed optimal growth at 10% NaCl. When grown in medium with an [NaCl] of 1.7 M, the internal cytoplasmic [Na+] and [Cl-] were 0.92 and 1.2 M, respectively, while K+ and Mg2+ concentrations in cells were 0.24 and 0.02 M, respectively. Intracellular [Na+] was fourfold higher than intracellular [K+]. Since Na+ and Cl- ions were not excluded from the cell, the influence of high salt concentrations on key enzyme activities was investigated in crude cell extracts. Activities greater than 60% of the maximal activity of the following key catabolic enzymes occurred at the following [NaCl] ranges: glyceraldehyde-3-phosphate dehydrogenase, 1 to 2 M; alcohol dehydrogenase (NAD linked), 2 to 4 M; pyruvate dehydrogenase, 0.5 to 1 M; and hydrogenase (methyl viologen linked), 0.5 to 3 M. These studies support the hypothesis that obligately halophilic, anaerobic eubacteria adapt to extreme salt concentrations differently than do halophilic, aerobic eubacteria, because they do not produce osmoregulants or exclude Cl-. This study also demonstrated that these halophilic, anaerobic eubacteria have a physiological similarity to archaebacterial halophiles, since Na+ and Cl- are present in high concentrations and are required for enzymatic activity.
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Selected References
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- Aitken D. M., Wicken A. J., Brown A. D. Properties of a halophil nicotinamide--adenine dinucleotide phosphate-specific isocitrate dehydrogenase. Preliminary studies of the salt relations and kinetics of the crude enzyme. Biochem J. 1970 Jan;116(1):125–134. doi: 10.1042/bj1160125. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BAXTER R. M. An interpretation of the effects of salts on the lactic dehydrogenase of Halobacterium salinarium. Can J Microbiol. 1959 Feb;5(1):47–57. doi: 10.1139/m59-006. [DOI] [PubMed] [Google Scholar]
- BAXTER R. M., GIBBONS N. E. Effects of sodium and potassium chloride on certain enzymes of Micrococcus halodenitrificans and Pseudomonas salinaria. Can J Microbiol. 1956 Oct;2(6):599–606. doi: 10.1139/m56-072. [DOI] [PubMed] [Google Scholar]
- BAXTER R. M., GIBBONS N. E. The glycerol dehydrogenases of Pseudomonas salinaria, Vibrio costicolus, and Escherichia coli in relation to bacterial halophilism. Can J Biochem Physiol. 1954 May;32(3):206–217. [PubMed] [Google Scholar]
- Bayley S. T., Morton R. A. Recent developments in the molecular biology of extremely halophilic bacteria. CRC Crit Rev Microbiol. 1978;6(2):151–205. doi: 10.3109/10408417809090622. [DOI] [PubMed] [Google Scholar]
- CHRISTIAN J. H., WALTHO J. A. Solute concentrations within cells of halophilic and non-halophilic bacteria. Biochim Biophys Acta. 1962 Dec 17;65:506–508. doi: 10.1016/0006-3002(62)90453-5. [DOI] [PubMed] [Google Scholar]
- Goodwin S., Zeikus J. G. Physiological adaptations of anaerobic bacteria to low pH: metabolic control of proton motive force in Sarcina ventriculi. J Bacteriol. 1987 May;169(5):2150–2157. doi: 10.1128/jb.169.5.2150-2157.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLMES P. K., HALVORSON H. O. PROPERTIES OF A PURIFIED HALOPHILIC MALIC DEHYDROGENASE. J Bacteriol. 1965 Aug;90:316–326. doi: 10.1128/jb.90.2.316-326.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Imhoff J. F., Rodriguez-Valera F. Betaine is the main compatible solute of halophilic eubacteria. J Bacteriol. 1984 Oct;160(1):478–479. doi: 10.1128/jb.160.1.478-479.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kashket E. R., Blanchard A. G., Metzger W. C. Proton motive force during growth of Streptococcus lactis cells. J Bacteriol. 1980 Jul;143(1):128–134. doi: 10.1128/jb.143.1.128-134.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kerby R., Niemczura W., Zeikus J. G. Single-carbon catabolism in acetogens: analysis of carbon flow in Acetobacterium woodii and Butyribacterium methylotrophicum by fermentation and 13C nuclear magnetic resonance measurement. J Bacteriol. 1983 Sep;155(3):1208–1218. doi: 10.1128/jb.155.3.1208-1218.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kushner D. J. Halophilic bacteria. Adv Appl Microbiol. 1968;10:73–99. doi: 10.1016/s0065-2164(08)70189-8. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lanyi J. K. Salt-dependent properties of proteins from extremely halophilic bacteria. Bacteriol Rev. 1974 Sep;38(3):272–290. doi: 10.1128/br.38.3.272-290.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lanyi J. K., Silverman M. P. The state of binding of intracellular K + in Halobacterium cutirubrum. Can J Microbiol. 1972 Jul;18(7):993–995. doi: 10.1139/m72-154. [DOI] [PubMed] [Google Scholar]
- Masui M., Wada S. Intracellular concentrations of Na+, K+, and cl minus of a moderately halophilic bacterium. Can J Microbiol. 1973 Oct;19(10):1181–1186. doi: 10.1139/m73-191. [DOI] [PubMed] [Google Scholar]
- Matheson A. T., Sprott G. D., McDonald I. J., Tessier H. Some properties of an unidentified halophile: growth characteristics, internal salt concentration, and morphology. Can J Microbiol. 1976 Jun;22(6):780–786. doi: 10.1139/m76-114. [DOI] [PubMed] [Google Scholar]
- Mullakhanbhai M. F., Larsen H. Halobacterium volcanii spec. nov., a Dead Sea halobacterium with a moderate salt requirement. Arch Microbiol. 1975 Aug 28;104(3):207–214. doi: 10.1007/BF00447326. [DOI] [PubMed] [Google Scholar]
- Ng T. K., Ben-Bassat A., Zeikus J. G. Ethanol Production by Thermophilic Bacteria: Fermentation of Cellulosic Substrates by Cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum. Appl Environ Microbiol. 1981 Jun;41(6):1337–1343. doi: 10.1128/aem.41.6.1337-1343.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Novitsky T. J., Kushner D. J. Influence of temperature and salt concentration on the growth of a facultatively halophilic "Micrococcus" sp. Can J Microbiol. 1975 Jan;21(1):107–110. doi: 10.1139/m75-017. [DOI] [PubMed] [Google Scholar]
- Pugh E. L., Wassef M. K., Kates M. Inhibition of fatty acid synthetase in Halobacterium cutirubrum and Escherichia coli by high salt concentrations. Can J Biochem. 1971 Aug;49(8):953–958. doi: 10.1139/o71-138. [DOI] [PubMed] [Google Scholar]
- Schink B., Lupton F. S., Zeikus J. G. Radioassay for hydrogenase activity in viable cells and documentation of aerobic hydrogen-consuming bacteria living in extreme environments. Appl Environ Microbiol. 1983 May;45(5):1491–1500. doi: 10.1128/aem.45.5.1491-1500.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stowe H. D., Braselton W. E., Kaneene J. B., Slanker M. Multielement assays of bovine tissue specimens by inductively coupled argon plasma emission spectroscopy. Am J Vet Res. 1985 Mar;46(3):561–565. [PubMed] [Google Scholar]
- Weimer P. J., Zeikus J. G. Acetate assimilation pathway of Methanosarcina barkeri. J Bacteriol. 1979 Jan;137(1):332–339. doi: 10.1128/jb.137.1.332-339.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeikus J. G., Fuchs G., Kenealy W., Thauer R. K. Oxidoreductases involved in cell carbon synthesis of Methanobacterium thermoautotrophicum. J Bacteriol. 1977 Nov;132(2):604–613. doi: 10.1128/jb.132.2.604-613.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]