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. 1983 May 1;96(5):1208–1217. doi: 10.1083/jcb.96.5.1208

Behavior of cells seeded in isolated fibronectin matrices

PMCID: PMC2112661  PMID: 6682421

Abstract

Cell-free fibronectin matrix (FN-matrix) isolated from chick embryo fibroblasts was used to study cell-matrix interaction. After 24 h, most fibroblastic cells, including those without cell surface fibronectin, adopted bipolar fusiform morphology. Cells grew in parallel arrays and aligned with each other apparently along FN-matrix. Since the orientation of fibronectin fibers was determined by chick embryo fibroblasts, our results suggested that intercellular organization of "matrix-using" cell type may be influenced by "matrix-producing" cell type. Whereas the elongation and alignment effects induced by FN-matrix have been detected in fibroblasts (both normal and transformed), myoblast, aortic endothelial cells, neural cell lines (B103 and RT4D1), and cardiac muscle cells, similar effects are not detected in bone marrow hemopoietic cells, circulating lymphocytic T and B cells, and sympathetic neurons. For epithelial cells, FN-matrix has varying effects. Elongation and alignment effects are detected only in transformed epithelial cells with a great reduction in keratin expression. The morphology of normal or transformed epithelial cells with abundant keratin appears unaffected by FN-matrix. FN-matrix reduced the growth of several transformed fibroblastic lines up to 25%, but did not restore the appearance of actin stress fibers and the normal migratory activities of Rous sarcoma virus-transformed rat cells.

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Selected References

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  1. Ali I. U., Mautner V., Lanza R., Hynes R. O. Restoration of normal morphology, adhesion and cytoskeleton in transformed cells by addition of a transformation-sensitive surface protein. Cell. 1977 May;11(1):115–126. doi: 10.1016/0092-8674(77)90322-1. [DOI] [PubMed] [Google Scholar]
  2. Birdwell C. R., Gospodarowicz D., Nicolson G. L. Identification, localization, and role of fibronectin in cultured bovine endothelial cells. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3273–3277. doi: 10.1073/pnas.75.7.3273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chen L. B. Alteration in cell surface LETS protein during myogenesis. Cell. 1977 Mar;10(3):393–400. doi: 10.1016/0092-8674(77)90026-5. [DOI] [PubMed] [Google Scholar]
  4. Chen L. B., Murray A., Segal R. A., Bushnell A., Walsh M. L. Studies on intercellular LETS glycoprotein matrices. Cell. 1978 Jun;14(2):377–391. doi: 10.1016/0092-8674(78)90123-x. [DOI] [PubMed] [Google Scholar]
  5. Ebendal T. Extracellular matrix fibrils and cell contacts in the chick embryo. Possible roles in orientation of cell migration and axon extension. Cell Tissue Res. 1977 Jan 4;175(4):439–458. doi: 10.1007/BF00222411. [DOI] [PubMed] [Google Scholar]
  6. Elsdale T., Bard J. Collagen substrata for studies on cell behavior. J Cell Biol. 1972 Sep;54(3):626–637. doi: 10.1083/jcb.54.3.626. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Folkman J., Moscona A. Role of cell shape in growth control. Nature. 1978 Jun 1;273(5661):345–349. doi: 10.1038/273345a0. [DOI] [PubMed] [Google Scholar]
  8. Greenburg G., Hay E. D. Epithelia suspended in collagen gels can lose polarity and express characteristics of migrating mesenchymal cells. J Cell Biol. 1982 Oct;95(1):333–339. doi: 10.1083/jcb.95.1.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hedman K., Kurkinen M., Alitalo K., Vaheri A., Johansson S., Hök M. Isolation of the pericellular matrix of human fibroblast cultures. J Cell Biol. 1979 Apr;81(1):83–91. doi: 10.1083/jcb.81.1.83. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hsieh P., Segal R., Chen L. B. Studies of fibronectin matrices in living cells with fluoresceinated gelatin. J Cell Biol. 1980 Oct;87(1):14–22. doi: 10.1083/jcb.87.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hynes R. O., Martin G. S., Shearer M., Critchley D. R., Epstein C. J. Viral transformation of rat myoblasts: effects on fusion and surface properties. Dev Biol. 1976 Jan;48(1):35–46. doi: 10.1016/0012-1606(76)90043-9. [DOI] [PubMed] [Google Scholar]
  12. Jaffe E. A., Mosher D. F. Synthesis of fibronectin by cultured human endothelial cells. J Exp Med. 1978 Jun 1;147(6):1779–1791. doi: 10.1084/jem.147.6.1779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnson L. V., Walsh M. L., Chen L. B. Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci U S A. 1980 Feb;77(2):990–994. doi: 10.1073/pnas.77.2.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. PORTER K. R., VANAMEE P. Observations on the formation of connective tissue fibers. Proc Soc Exp Biol Med. 1949 Aug;71(4):513–516. doi: 10.3181/00379727-71-17241. [DOI] [PubMed] [Google Scholar]
  15. Rein A., Rubin H. Effects of local cell concentrations upon the growth of chick embryo cells in tissue culture. Exp Cell Res. 1968 Mar;49(3):666–678. doi: 10.1016/0014-4827(68)90213-9. [DOI] [PubMed] [Google Scholar]
  16. Rovasio R. A., Delouvee A., Yamada K. M., Timpl R., Thiery J. P. Neural crest cell migration: requirements for exogenous fibronectin and high cell density. J Cell Biol. 1983 Feb;96(2):462–473. doi: 10.1083/jcb.96.2.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Summerhayes I. C., Chen L. B. Localization of a Mr 52,000 keratin in basal epithelial cells of the mouse bladder and expression throughout neoplastic progression. Cancer Res. 1982 Oct;42(10):4098–4109. [PubMed] [Google Scholar]
  18. Summerhayes I. C., Cheng Y. S., Sun T. T., Chen L. B. Expression of keratin and vimentin intermediate filaments in rabbit bladder epithelial cells at different stages of benzo[a]pyrene-induced neoplastic progression. J Cell Biol. 1981 Jul;90(1):63–69. doi: 10.1083/jcb.90.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Summerhayes I. C., Lampidis T. J., Bernal S. D., Nadakavukaren J. J., Nadakavukaren K. K., Shepherd E. L., Chen L. B. Unusual retention of rhodamine 123 by mitochondria in muscle and carcinoma cells. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5292–5296. doi: 10.1073/pnas.79.17.5292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Thiery J. P., Duband J. L., Delouvée A. Pathways and mechanisms of avian trunk neural crest cell migration and localization. Dev Biol. 1982 Oct;93(2):324–343. doi: 10.1016/0012-1606(82)90121-x. [DOI] [PubMed] [Google Scholar]
  21. Tsen S. D., Lee R., Damiani B., Hsieh P., Chen L. B. F-actin inhibits protein kinase activity associated with the src-gene product. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 2):967–974. doi: 10.1101/sqb.1980.044.01.104. [DOI] [PubMed] [Google Scholar]
  22. Vlodavsky I., Lui G. M., Gospodarowicz D. Morphological appearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic. Cell. 1980 Mar;19(3):607–616. doi: 10.1016/s0092-8674(80)80037-7. [DOI] [PubMed] [Google Scholar]
  23. Wartiovaara J., Linder E., Ruoslahti E., Vaheri A. Distribution of fibroblast surface antigen: association with fibrillar structures of normal cells and loss upon viral transformation. J Exp Med. 1974 Dec 1;140(6):1522–1533. doi: 10.1084/jem.140.6.1522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Willingham M. C., Yamada K. M., Yamada S. S., Pouysségur J., Pastan I. Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts. Cell. 1977 Mar;10(3):375–380. doi: 10.1016/0092-8674(77)90024-1. [DOI] [PubMed] [Google Scholar]
  25. Yamada K. M., Olden K., Pastan I. Transformation-sensitive cell surface protein: isolation, characterization, and role in cellular morphology and adhesion. Ann N Y Acad Sci. 1978 Jun 20;312:256–277. doi: 10.1111/j.1749-6632.1978.tb16807.x. [DOI] [PubMed] [Google Scholar]
  26. Yamada K. M., Yamada S. S., Pastan I. Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1217–1221. doi: 10.1073/pnas.73.4.1217. [DOI] [PMC free article] [PubMed] [Google Scholar]

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