Abstract
The endogenous lectin of Xenopus laevis oocytes, unfertilized eggs, and blastula-stage embryos was immunohistochemically localized using a highly specific antiserum. Each tissue was examined with several techniques, including paraformaldehyde or glutaraldehyde fixation, frozen or plastic sections, and immunofluorescence or immunoperoxidase staining. In oocytes and unfertilized eggs, lectin was detected in association with yolk platelets, cortical granules, and the vitelline envelope. In embryos, cortical granules had disappeared and lectin was found in the cleavage furrows between the embryonic cells. The distribution of the lectin suggests that it plays more than one role in this developing system.
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barondes S. H., Cooper D. N., Haywood-Reid P. L. Discoidin I and discoidin II are localized differently in developing Dictyostelium discoideum. J Cell Biol. 1983 Jan;96(1):291–296. doi: 10.1083/jcb.96.1.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barondes S. H., Haywood-Reid P. L. Externalization of an endogenous chicken muscle lectin with in vivo development. J Cell Biol. 1981 Nov;91(2 Pt 1):568–572. doi: 10.1083/jcb.91.2.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barondes S. H. Lectins: their multiple endogenous cellular functions. Annu Rev Biochem. 1981;50:207–231. doi: 10.1146/annurev.bi.50.070181.001231. [DOI] [PubMed] [Google Scholar]
- Beyer E. C., Barondes S. H. Quantitation of two endogenous lactose-inhibitable lectins in embryonic and adult chicken tissues. J Cell Biol. 1982 Jan;92(1):23–27. doi: 10.1083/jcb.92.1.23. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beyer E. C., Barondes S. H. Secretion of endogenous lectin by chicken intestinal goblet cells. J Cell Biol. 1982 Jan;92(1):28–33. doi: 10.1083/jcb.92.1.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dumont J. N. Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals. J Morphol. 1972 Feb;136(2):153–179. doi: 10.1002/jmor.1051360203. [DOI] [PubMed] [Google Scholar]
- Hawkes R., Niday E., Gordon J. A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982 Jan 1;119(1):142–147. doi: 10.1016/0003-2697(82)90677-7. [DOI] [PubMed] [Google Scholar]
- Roberson M. M., Armstrong P. B. Carbohydrate-binding component of amphibian embryo cell surfaces: restriction to surface regions capable of cell adhesion. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3460–3463. doi: 10.1073/pnas.77.6.3460. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberson M. M., Armstrong P. B. Regional segregation of ConA receptors on dissociated amphibian embryo cells. Exp Cell Res. 1979 Aug;122(1):23–29. doi: 10.1016/0014-4827(79)90556-1. [DOI] [PubMed] [Google Scholar]
- Roberson M. M., Barondes S. H. Lectin from embryos and oocytes of Xenopus laevis. Purification and properties. J Biol Chem. 1982 Jul 10;257(13):7520–7524. [PubMed] [Google Scholar]
- Sakakibara F., Kawauchi H., Takayanagi G., Ise H. Egg lectin of Rana japonica and its receptor glycoprotein of Ehrlich tumor cells. Cancer Res. 1979 Apr;39(4):1347–1352. [PubMed] [Google Scholar]
- Sakakibara F., Takayanagi G., Kawauchi H., Watanabe K., Hakomori S. An anti-A-like lectin of Rana catesbiana eggs showing unusual reactivity. Biochim Biophys Acta. 1976 Sep 24;444(2):386–395. doi: 10.1016/0304-4165(76)90382-2. [DOI] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wyrick R. E., Nishihara T., Hedrick J. L. Agglutination of jelly coat and cortical granule components and the block to polyspermy in the amphibian Xenopus laevis. Proc Natl Acad Sci U S A. 1974 May;71(5):2067–2071. doi: 10.1073/pnas.71.5.2067. [DOI] [PMC free article] [PubMed] [Google Scholar]