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. 1981 Mar 1;88(3):536–542. doi: 10.1083/jcb.88.3.536

Glucocorticoid regulation of amino acid transport in anucleate rat hepatoma (HTC) cells

PMCID: PMC2112759  PMID: 7217203

Abstract

The transport of alpha-aminoisobutyric acid (AIB) by rat hepatoma tissue culture (HTC) cells is rapidly and reversibly inhibited by dexamethasone and other glucocorticoids. To investigate the role of the nucleus in the regulation of transport and to determine whether steroid hormones or steroid-receptor complexes may have direct effects on cytoplasmic or membrane functions, we have examined the regulation of transport by dexamethasone in anucleate HTC cells. Cytoplasts prepared from suspension cultures of HTC cells fully retain active transport of AIB with the same kinetic properties as intact cells. However, the uptake of AIB is not inhibited by dexamethasone or other corticosteroids. Neither is the inhibited rate of transport, manifested by cytoplasts prepared from dexamethasone-treated cells, restored to normal upon removal of the hormone. Anucleate cells exhibit specific, saturable binding of [3H]dexamethasone; however, the binding is reduced compared with that of intact cells. The nucleus is thus required for the glucocorticoid regulation of amino acid transport in HTC cells.

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Selected References

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  1. Barouski P. A., Gelehrter T. D. Loss of glucocorticoid regulation of plasminogen activator activity in anucleate rat hepatoma cells. Biochem Biophys Res Commun. 1980 Oct 31;96(4):1540–1546. doi: 10.1016/0006-291x(80)91349-2. [DOI] [PubMed] [Google Scholar]
  2. Baulieu E. E., Godeau F., Schorderet M., Schorderet-Slatkine S. Steroid-induced meiotic division in Xenopus laevis oocytes: surface and calcium. Nature. 1978 Oct 19;275(5681):593–598. doi: 10.1038/275593a0. [DOI] [PubMed] [Google Scholar]
  3. Baumann H., Gelehrter T. D., Doyle D. Dexamethasone regulates the program of secretory glycoprotein synthesis in hepatoma tissue culture cells. J Cell Biol. 1980 Apr;85(1):1–8. doi: 10.1083/jcb.85.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Butcher F. R., Perdue J. F. Cytochalasin B: effect on hormone-mediated responses in cultured cells. J Cell Biol. 1973 Mar;56(3):857–861. doi: 10.1083/jcb.56.3.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ebstensen R. D., Plagemann P. G. Cytochalasin B: inhibition of glucose and glucosamine transport. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1430–1434. doi: 10.1073/pnas.69.6.1430. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Epstein D., Elias-Bishko S., Hershko A. Requirement for protein synthesis in the regulation of protein breakdown in cultured hepatoma cells. Biochemistry. 1975 Nov 18;14(23):5199–5204. doi: 10.1021/bi00694a028. [DOI] [PubMed] [Google Scholar]
  7. Fan W. W., Ivarie R. D., Levinson B. B. Nucleus-dependent regulation of tyrosine aminotransferase degradation in hepatoma tissue culture cells. J Biol Chem. 1977 Nov 10;252(21):7834–7841. [PubMed] [Google Scholar]
  8. Gelehrter T. D., Emanuel J. R. Hormonal regulation of protein degradation in hepatoma cells in tissue culture. Endocrinology. 1974 Mar;94(3):676–684. doi: 10.1210/endo-94-3-676. [DOI] [PubMed] [Google Scholar]
  9. Gopalakrishnan T. V., Thompson E. B. The stability of tyrosine aminotransferase and other proteins in enucleated rat hepatoma tissue culture cells. J Cell Physiol. 1977 Oct;93(1):69–79. doi: 10.1002/jcp.1040930110. [DOI] [PubMed] [Google Scholar]
  10. Heaton J. H., Gelehrter T. D. Derepression of amino acid transport by amino acid starvation in rat hepatoma cells. J Biol Chem. 1977 May 10;252(9):2900–2907. [PubMed] [Google Scholar]
  11. Ivarie R. D., Fan W. J., Tomkins G. M. Analysis of the induction and deinduction of tyrosine aminotransferase in enucleated HTC cells. J Cell Physiol. 1975 Apr;85(2 Pt 2 Suppl 1):357–364. doi: 10.1002/jcp.1040850404. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Liang T., Liao S. A very rapid effect of androgen on initiation of protein synthesis in prostate. Proc Natl Acad Sci U S A. 1975 Feb;72(2):706–709. doi: 10.1073/pnas.72.2.706. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MacDonald R. G., Cidlowski J. A. Alterations in specificity of the glucocorticoid receptor with temperature in rat splenic lymphocytes. J Steroid Biochem. 1979 Jan;10(1):21–29. doi: 10.1016/0022-4731(79)90136-5. [DOI] [PubMed] [Google Scholar]
  15. McDonald R. A., Gelehrter T. D. Gllcocorticoid inhibition of amino acid transport in rat hepatoma cells. Biochem Biophys Res Commun. 1977 Oct 24;78(4):1304–1310. doi: 10.1016/0006-291x(77)91434-6. [DOI] [PubMed] [Google Scholar]
  16. Mizel S. B., Wilson L. Inhibition of the transport of several hexoses in mammalian cells by cytochalasin B. J Biol Chem. 1972 Jun 25;247(12):4102–4105. [PubMed] [Google Scholar]
  17. Reichberg S. B., Gelehrter T. D. Glucocorticoid inhibition of two discrete glycine transport systems in rat hepatoma cells. J Biol Chem. 1980 Jun 25;255(12):5708–5714. [PubMed] [Google Scholar]
  18. Risser W. L., Gelehrter T. D. Hormonal modulation of amino acid transport in rat hepatoma cells in tissue culture. J Biol Chem. 1973 Feb 25;248(4):1248–1254. [PubMed] [Google Scholar]
  19. Sibley C. H., Tomkins G. M. Mechanisms of steroid resistance. Cell. 1974 Aug;2(4):221–227. doi: 10.1016/0092-8674(74)90014-2. [DOI] [PubMed] [Google Scholar]

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