Abstract
Intact erythrocytes become immediately crenated upon addition of 2,4- dinitrophenol (DNP) or pyrenebutyric acid (PBA). However, when cells are incubated at 37 degrees C in the presence of the crenating agents with glucose, they gradually (4--8 h) recover the normal biconcave disc form. The recovery process does not reflect a gradual inactivation of DNP or PBA since fresh cells are equally crenated by the supernatant from the recovered cells. Further, after recovery and removal of the crenating agents, cells are found to be desensitized to the readdition of DNP as well as to the addition of PBA, but they are more sensitive to cupping by chlorpromazine. This alteration in the cell membrane responsiveness was reversible upon further incubation in the absence of DNP. Recovery is dependent upon cellular metabolic state since an energy source is needed and incubation with guanosine but not adenosine will accelerate conversion to the disc shape. It is suggested that the conversion of cells from crenated to disc shape in the presence of the crenators, represents an alteration or rearrangement of membrane components rather than a redistribution of the crenators within the membrane. This shape recovery process may be important for erythrocyte shape preservation as well as shape control in other cells.
Full Text
The Full Text of this article is available as a PDF (559.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allan D., Michell R. H. A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytes. Biochim Biophys Acta. 1978 Apr 4;508(2):277–286. doi: 10.1016/0005-2736(78)90330-9. [DOI] [PubMed] [Google Scholar]
- Beck J. S. Relations between membrane monolayers in some red cell shape transformations. J Theor Biol. 1978 Dec 21;75(4):487–501. doi: 10.1016/0022-5193(78)90358-2. [DOI] [PubMed] [Google Scholar]
- Ben-Ze'ev A., Farmer S. R., Penman S. Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts. Cell. 1980 Sep;21(2):365–372. doi: 10.1016/0092-8674(80)90473-0. [DOI] [PubMed] [Google Scholar]
- Bertles J. F., Milner P. F. Irreversibly sickled erythrocytes: a consequence of the heterogeneous distribution of hemoglobin types in sickle-cell anemia. J Clin Invest. 1968 Aug;47(8):1731–1741. doi: 10.1172/JCI105863. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deuticke B. Transformation and restoration of biconcave shape of human erythrocytes induced by amphiphilic agents and changes of ionic environment. Biochim Biophys Acta. 1968 Dec 10;163(4):494–500. doi: 10.1016/0005-2736(68)90078-3. [DOI] [PubMed] [Google Scholar]
- Hoffman J. F. Quantitative study of factors which control shape transformations of human red blood cells of constant volume. Nouv Rev Fr Hematol. 1972 Nov-Dec;12(6):771–774. [PubMed] [Google Scholar]
- LOCK S. P., SMITH R. S., HARDISTY R. M. Stomatocytosis: a hereditary red cell anomally associated with haemolytic anaemia. Br J Haematol. 1961 Jul;7:303–314. doi: 10.1111/j.1365-2141.1961.tb00341.x. [DOI] [PubMed] [Google Scholar]
- Matayoshi E. D. Distribution of shape-changing compounds across the red cell membrane. Biochemistry. 1980 Jul 22;19(15):3414–3422. doi: 10.1021/bi00556a002. [DOI] [PubMed] [Google Scholar]
- Quist E. E., Reece K. L. The role of diphosphatidylinositol in erythrocyte membrane shape regulation. Biochem Biophys Res Commun. 1980 Aug 14;95(3):1023–1030. doi: 10.1016/0006-291x(80)91575-2. [DOI] [PubMed] [Google Scholar]
- SINGER K., FISHER B., PERLSTEIN M. A. Acanthrocytosis; a genetic erythrocytic malformation. Blood. 1952 Jun;7(6):577–591. [PubMed] [Google Scholar]
- Sheetz M. P., Singer S. J. Biological membranes as bilayer couples. A molecular mechanism of drug-erythrocyte interactions. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4457–4461. doi: 10.1073/pnas.71.11.4457. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sheetz M. P., Singer S. J. Equilibrium and kinetic effects of drugs on the shapes of human erythrocytes. J Cell Biol. 1976 Jul;70(1):247–251. doi: 10.1083/jcb.70.1.247. [DOI] [PMC free article] [PubMed] [Google Scholar]