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. 1988 Jul;170(7):3294–3296. doi: 10.1128/jb.170.7.3294-3296.1988

Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

D E Shevell 1, A M Abou-Zamzam 1, B Demple 1, G C Walker 1
PMCID: PMC211286  PMID: 3290202

Abstract

We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.

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Selected References

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