Abstract
Various deletions were introduced into the central region of Escherichia coli flagellin (497 residues) without destroying its ability to form flagellar filaments. The smallest flagellin retained only the N-terminal 193 residues and the C-terminal 117 residues, which are suggested to be the domains essential for filament formation.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chang J. Y., Brown D. M., Glazer A. N. Characterization of the subunits of the flagella of Proteus vulgaris. J Biol Chem. 1969 Oct 10;244(19):5196–5200. [PubMed] [Google Scholar]
- DeLange R. J., Chang J. Y., Shaper J. H., Glazer A. N. Amino acid sequence of flagellin of Bacillus subtilis 168. III. Tryptic peptides, N-bromosuccinimide peptides, and the complete amino acid sequence. J Biol Chem. 1976 Feb 10;251(3):705–711. [PubMed] [Google Scholar]
- Delange R. J., Chang J. Y., Shaper J. H., Martinez R. J., Komatsu S. K., Glazer A. N. On the amino-acid sequence of flagellin from Bacillus subtilis 168: comparison with other bacterial flagellins. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3428–3431. doi: 10.1073/pnas.70.12.3428. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gill P. R., Agabian N. The nucleotide sequence of the Mr = 28,500 flagellin gene of Caulobacter crescentus. J Biol Chem. 1983 Jun 25;258(12):7395–7401. [PubMed] [Google Scholar]
- Homma M., Fujita H., Yamaguchi S., Iino T. Excretion of unassembled flagellin by Salmonella typhimurium mutants deficient in hook-associated proteins. J Bacteriol. 1984 Sep;159(3):1056–1059. doi: 10.1128/jb.159.3.1056-1059.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Homma M., Fujita H., Yamaguchi S., Iino T. Regions of Salmonella typhimurium flagellin essential for its polymerization and excretion. J Bacteriol. 1987 Jan;169(1):291–296. doi: 10.1128/jb.169.1.291-296.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Homma M., Iino T., Kutsukake K., Yamaguchi S. In vitro reconstitution of flagellar filaments onto hooks of filamentless mutants of Salmonella typhimurium by addition of hook-associated proteins. Proc Natl Acad Sci U S A. 1986 Aug;83(16):6169–6173. doi: 10.1073/pnas.83.16.6169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Homma M., Iino T. Locations of hook-associated proteins in flagellar structures of Salmonella typhimurium. J Bacteriol. 1985 Apr;162(1):183–189. doi: 10.1128/jb.162.1.183-189.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Homma M., Kutsukake K., Iino T., Yamaguchi S. Hook-associated proteins essential for flagellar filament formation in Salmonella typhimurium. J Bacteriol. 1984 Jan;157(1):100–108. doi: 10.1128/jb.157.1.100-108.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. doi: 10.1146/annurev.ge.11.120177.001113. [DOI] [PubMed] [Google Scholar]
- Joys T. M. The covalent structure of the phase-1 flagellar filament protein of Salmonella typhimurium and its comparison with other flagellins. J Biol Chem. 1985 Dec 15;260(29):15758–15761. [PubMed] [Google Scholar]
- Kondoh H., Ozeki H. Deletion and amber mutants of fla loci in Escherichia coli K-12. Genetics. 1976 Nov;84(3):403–421. doi: 10.1093/genetics/84.3.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krupski G., Götz R., Ober K., Pleier E., Schmitt R. Structure of complex flagellar filaments in Rhizobium meliloti. J Bacteriol. 1985 Apr;162(1):361–366. doi: 10.1128/jb.162.1.361-366.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuwajima G., Asaka J., Fujiwara T., Fujiwara T., Node K., Kondo E. Nucleotide sequence of the hag gene encoding flagellin of Escherichia coli. J Bacteriol. 1986 Dec;168(3):1479–1483. doi: 10.1128/jb.168.3.1479-1483.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuwajima G. Flagellin domain that affects H antigenicity of Escherichia coli K-12. J Bacteriol. 1988 Jan;170(1):485–488. doi: 10.1128/jb.170.1.485-488.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Martinez R. J., Brown D. M., Glazer A. N. The formation of bacterial flagella. 3. Characterization of the subunits of the flagella of Bacillus subtilis and Spirillum serpens. J Mol Biol. 1967 Aug 28;28(1):45–51. doi: 10.1016/s0022-2836(67)80076-7. [DOI] [PubMed] [Google Scholar]
- Maruyama M., Lodderstaedt G., Schmitt R. Purification and biochemical properties of complex flagella isolated from Rhizobium lupini H13-3. Biochim Biophys Acta. 1978 Jul 21;535(1):110–124. doi: 10.1016/0005-2795(78)90038-7. [DOI] [PubMed] [Google Scholar]
- Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. doi: 10.1016/0076-6879(83)01005-8. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shirakihara Y., Wakabayashi T. Three-dimensional image reconstruction of straight flagella from a mutant Salmonella typhimurium. J Mol Biol. 1979 Jul 5;131(3):485–507. doi: 10.1016/0022-2836(79)90004-4. [DOI] [PubMed] [Google Scholar]
- Silverman M., Simon M. I. Bacterial flagella. Annu Rev Microbiol. 1977;31:397–419. doi: 10.1146/annurev.mi.31.100177.002145. [DOI] [PubMed] [Google Scholar]
- Trachtenberg S., DeRosier D. J. Three-dimensional structure of the frozen-hydrated flagellar filament. The left-handed filament of Salmonella typhimurium. J Mol Biol. 1987 Jun 5;195(3):581–601. doi: 10.1016/0022-2836(87)90184-7. [DOI] [PubMed] [Google Scholar]
- Wei L. N., Joys T. M. Covalent structure of three phase-1 flagellar filament proteins of Salmonella. J Mol Biol. 1985 Dec 20;186(4):791–803. doi: 10.1016/0022-2836(85)90397-3. [DOI] [PubMed] [Google Scholar]
- Yamaguchi S., Fujita H., Sugata K., Taira T., Iino T. Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella. J Gen Microbiol. 1984 Feb;130(2):255–265. doi: 10.1099/00221287-130-2-255. [DOI] [PubMed] [Google Scholar]
- Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]