Skip to main content
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1982 Nov 1;95(2):626–631. doi: 10.1083/jcb.95.2.626

Modification of cell surface glycoprotein: addition of fucosyl residues during epidermal differentiation

PMCID: PMC2112967  PMID: 6292241

Abstract

When cutaneous sections from the newborn rat were treated with alpha- fucosidase, Ulex europeus agglutinin I (UEA) binding to the cell surface of the differentiated cells in the epidermis was diminished and there was an appearance in these cell layers of binding by Bandeiraea simplicifolia I-B4 lectin (BS I-B4), which normally is specific for the basal cells. A similar treatment with alpha-galactosidase resulted in a loss of BS I-B4 binding, but had no effect on UEA binding. Glycoproteins isolated from the membranes of epidermal cells showed a threefold increase in the ratio of binding to UEA versus BS I-B4 affinity columns as the proteins were derived from the more differentiated cell populations. These data suggest that alpha-fucosyl residues are added to the glycoproteins on the cell surfaces of differentiated cells, thus blocking alpha-galactosyl residues and changing the lectin binding specificity as epidermal cells move out of the basal cell layer.

Full Text

The Full Text of this article is available as a PDF (1.9 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Augener W., Brittinger G., Abel C. A., Goldblum N. Sequential expression of fucosyltransferase and N-acetylneuraminyltransferase activities in human leukemic cells arrested at different stages of maturation. Cancer Biochem Biophys. 1980;5(1):33–39. [PubMed] [Google Scholar]
  2. Blake D. A., Goldstein I. J. Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography. Anal Biochem. 1980 Feb;102(1):103–109. doi: 10.1016/0003-2697(80)90324-3. [DOI] [PubMed] [Google Scholar]
  3. Brabec R. K., Peters B. P., Bernstein I. A., Gray R. H., Goldstein I. J. Differential lectin binding to cellular membranes in the epidermis of the newborn rat. Proc Natl Acad Sci U S A. 1980 Jan;77(1):477–479. doi: 10.1073/pnas.77.1.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brabec R. K., Peters B. P., Bernstein I. A., Gray R. H., Goldstein I. J. Differential lectin binding to cellular membranes in the epidermis of the newborn rat. Proc Natl Acad Sci U S A. 1980 Jan;77(1):477–479. doi: 10.1073/pnas.77.1.477. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Braulke T., Biesold D. Developmental patterns of galactosyltransferase activity in various regions of rat brain. J Neurochem. 1981 Mar;36(3):1289–1291. doi: 10.1111/j.1471-4159.1981.tb01731.x. [DOI] [PubMed] [Google Scholar]
  6. Breuer W., Siu C. H. Identification of endogenous binding proteins for the lectin discoidin-I in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2115–2119. doi: 10.1073/pnas.78.4.2115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brewer J. M., Ashworth R. B. Disc electrophoresis. J Chem Educ. 1969 Jan;46(1):41–45. doi: 10.1021/ed046p41. [DOI] [PubMed] [Google Scholar]
  8. Duksin D., Bornstein P. Impaired conversion of procollagen to collagen by fibroblasts and bone treated with tunicamycin, an inhibitor of protein glycosylation. J Biol Chem. 1977 Feb 10;252(3):955–962. [PubMed] [Google Scholar]
  9. Essner E., Schreiber J., Griewski R. A. Localization of carbohydrate components in rat colon with fluoresceinated lectins. J Histochem Cytochem. 1978 Jun;26(6):452–458. doi: 10.1177/26.6.670681. [DOI] [PubMed] [Google Scholar]
  10. Etzler M. E., Branstrator M. L. Differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectins. J Cell Biol. 1974 Aug;62(2):329–343. doi: 10.1083/jcb.62.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Firestone G. L., Heath E. C. Role of protein glycosylation in the cAMP-mediated induction of alkaline phosphatase in mouse L-cells. J Biol Chem. 1981 Feb 10;256(3):1404–1411. [PubMed] [Google Scholar]
  12. Freeman H. J., Lotan R., Kim Y. S. Application of lectins for detection of goblet cell glycoconjugate differences in proximal and distal colon of the rat. Lab Invest. 1980 Apr;42(4):405–412. [PubMed] [Google Scholar]
  13. Gahmberg C. G., Hakomori S. I. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem. 1973 Jun 25;248(12):4311–4317. [PubMed] [Google Scholar]
  14. Geltosky J. E., Siu C. H., Lerner R. A. Glycoproteins of the plasma membrane of Dictyostelium discoideum during development. Cell. 1976 Jul;8(3):391–396. doi: 10.1016/0092-8674(76)90151-3. [DOI] [PubMed] [Google Scholar]
  15. Gooi H. C., Feizi T., Kapadia A., Knowles B. B., Solter D., Evans M. J. Stage-specific embryonic antigen involves alpha 1 goes to 3 fucosylated type 2 blood group chains. Nature. 1981 Jul 9;292(5819):156–158. doi: 10.1038/292156a0. [DOI] [PubMed] [Google Scholar]
  16. Hashimoto K., King L. E., Jr, Yamanishi Y., Beachey E. H., Maeyens E. Identification of the substance binding pemphigus antibody and concanavalin A in the skin. J Invest Dermatol. 1974 Apr;62(4):423–435. doi: 10.1111/1523-1747.ep12701675. [DOI] [PubMed] [Google Scholar]
  17. Heifetz A., Lennarz W. J. Biosynthesis of N-glycosidically linked glycoproteins during gastrulation of sea urchin embryos. J Biol Chem. 1979 Jul 10;254(13):6119–6127. [PubMed] [Google Scholar]
  18. Hickman S., Kulczycki A., Jr, Lynch R. G., Kornfeld S. Studies of the mechanism of tunicamycin in hibition of IgA and IgE secretion by plasma cells. J Biol Chem. 1977 Jun 25;252(12):4402–4408. [PubMed] [Google Scholar]
  19. Holt P. J., Anglin J. H., Jr, Nordquist R. E. Localization of specific carbohydrate configurations in human skin using fluorescein-labelled lectins. Br J Dermatol. 1979 Mar;100(3):237–245. doi: 10.1111/j.1365-2133.1979.tb06194.x. [DOI] [PubMed] [Google Scholar]
  20. Leavitt R., Schlesinger S., Kornfeld S. Impaired intracellular migration and altered solubility of nonglycosylated glycoproteins of vesicular stomatitis virus and Sindbis virus. J Biol Chem. 1977 Dec 25;252(24):9018–9023. [PubMed] [Google Scholar]
  21. Maylié-Pfenninger M. F., Jamieson J. D. Development of cell surface saccharides on embryonic pancreatic cells. J Cell Biol. 1980 Jul;86(1):96–103. doi: 10.1083/jcb.86.1.96. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Miyagawa T. Heterogeneity and some properties of beta-galactosidase from newborn rat epidermis. J Invest Dermatol. 1979 Oct;73(4):285–287. doi: 10.1111/1523-1747.ep12531662. [DOI] [PubMed] [Google Scholar]
  23. Miyagawa T. Multiple forms of epidermal alpha-fucosidase. J Invest Dermatol. 1979 Oct;73(4):288–290. doi: 10.1111/1523-1747.ep12531696. [DOI] [PubMed] [Google Scholar]
  24. Murphy L. A., Goldstein I. J. Five alpha-D-galactopyranosyl-binding isolectins from Bandeiraea simplicifolia seeds. J Biol Chem. 1977 Jul 10;252(13):4739–4742. [PubMed] [Google Scholar]
  25. Nieland M. L. Epidermal intercellular staining with fluorescein-conjugated phytohemagglutinins. J Invest Dermatol. 1973 Feb;60(2):61–66. doi: 10.1111/1523-1747.ep12724137. [DOI] [PubMed] [Google Scholar]
  26. Olden K., Pratt R. M., Yamada K. M. Role of carbohydrates in protein secretion and turnover: effects of tunicamycin on the major cell surface glycoprotein of chick embryo fibroblasts. Cell. 1978 Mar;13(3):461–473. doi: 10.1016/0092-8674(78)90320-3. [DOI] [PubMed] [Google Scholar]
  27. Raedler E., Raedler A., Feldhaus S. Varying expressions of lectin receptors within embryonic cell layers of murine cerebral cortex. Anat Embryol (Berl) 1981;162(1):21–28. doi: 10.1007/BF00318091. [DOI] [PubMed] [Google Scholar]
  28. Reisner Y., Linker-Israeli M., Sharon N. Separation of mouse thymocytes into two subpopulations by the use of peanut agglutinin. Cell Immunol. 1976 Jul;25(1):129–134. doi: 10.1016/0008-8749(76)90103-9. [DOI] [PubMed] [Google Scholar]
  29. Reisner Y., Ravid A., Sharon N. Use of soybean agglutinin for the separation of mouse B and T lymphocytes. Biochem Biophys Res Commun. 1976 Oct 18;72(4):1585–1591. doi: 10.1016/s0006-291x(76)80195-7. [DOI] [PubMed] [Google Scholar]
  30. Reisner Y., Sharon N., Haran-Ghera N. Expression of peanut agglutinin receptors on virus-induced preleukemic cells in mice. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2244–2246. doi: 10.1073/pnas.77.4.2244. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sibrack L. A., Gray R. H., Bernstein I. A. Localization of the histidine-rich protein in keratohyalin: a morphologic and macromolecular marker in epidermal differentiation. J Invest Dermatol. 1974 Apr;62(4):394–405. doi: 10.1111/1523-1747.ep12701654. [DOI] [PubMed] [Google Scholar]
  32. Surani M. A. Glycoprotein synthesis and inhibition of glycosylation by tunicamycin in preimplantation mouse embryos: compaction and trophoblast adhesion. Cell. 1979 Sep;18(1):217–227. doi: 10.1016/0092-8674(79)90370-2. [DOI] [PubMed] [Google Scholar]
  33. Ullrich S. J., Kent C., Carlson D. M. Changes in galactosyltransferase activity in chick pectoral muscle during embryonic development. Biochem J. 1981 Apr 15;196(1):17–23. doi: 10.1042/bj1960017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Van Lis J. M., Kalsbeek G. L. The interaction of Concanavalin A and the surface coat of stratified squamous epithelium. Br J Dermatol. 1975 Jan;92(1):27–35. doi: 10.1111/j.1365-2133.1975.tb03029.x. [DOI] [PubMed] [Google Scholar]
  35. Vaughan F. L., Bernstein I. A. Studies of proliferative capabilities in isolated epidermal basal and differentiated cells. J Invest Dermatol. 1971 Jun;56(6):454–466. doi: 10.1111/1523-1747.ep12261386. [DOI] [PubMed] [Google Scholar]
  36. West C. M., McMahon D., Molday R. S. Identification of glycoproteins, using lectins as probes, in plasma membranes from Dictyostelium discoideum and human erythrocytes. J Biol Chem. 1978 Mar 10;253(5):1716–1724. [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES