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The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1982 Nov 1;95(2):619–625. doi: 10.1083/jcb.95.2.619

High-resolution mapping of satellite DNA using biotin-labeled DNA probes

PMCID: PMC2112973  PMID: 6754749

Abstract

We have developed a novel method for high resolution mapping of specific DNA sequences after in situ hybridization. DNA probes, labeled with biotin-nucleotides in conventional nick-translation reactions, are hybridized to cytological preparations and detected with affinity- purified rabbit antibiotin antibodies followed by antibodies to rabbit IgG that are conjugated to fluorescent or enzymatic reagents. Using peroxidase labeled anti-rabbit IgG, we are able to detect and localize specific sequences at both the light and electron microscopic levels. Initial studies were done with repeated DNA sequences previously mapped by light microscope autoradiography to assess the fidelity and resolution of this method. An analysis using biotin-labeled mouse satellite DNA is presented here.

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Selected References

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