Abstract
We have used a new method to extensively modify the redox state of the plastoquinone pool in Chlamydomonas reinhardtii intact cells. This was achieved by an anaerobic treatment that inhibits the chlororespiratory pathway recently described by P. Bennoun (Proc. Natl. Acad. Sci. USA, 1982, 79:4352-4356). A state I (plus 3,4-dichlorophenyl-1,1- dimethylurea) leads to anaerobic state transition induced a decrease in the maximal fluorescence yield at room temperature and in the FPSII/FPSI ratio at 77 degrees K, which was three times larger than in a classical state I leads to state II transition. The fluorescence changes observed in vivo were similar in amplitude to those observed in vitro upon transfer to the light of dark-adapted, broken chloroplasts incubated in the presence of ATP. We then compared the phosphorylation pattern of thylakoid polypeptides in C. reinhardtii in vitro and in vivo using gamma-[32P]ATP and [32P]orthophosphate labeling, respectively. The same set of polypeptides, mainly light-harvesting complex polypeptides, was phosphorylated in both cases. We observed that this phosphorylation process is reversible and is mediated by the redox state of the plastoquinone pool in vivo as well as in vitro. Similar changes of even larger amplitude were observed with the F34 mutant intact cells lacking in photosystem II centers. The presence of the photosystem II centers is then not required for the occurrence of the plastoquinone-mediated phosphorylation of light-harvesting complex polypeptides.
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Selected References
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- Bennett J. Chloroplast phosphoproteins. Evidence for a thylakoid-bound phosphoprotein phosphatase. Eur J Biochem. 1980 Feb;104(1):85–89. doi: 10.1111/j.1432-1033.1980.tb04403.x. [DOI] [PubMed] [Google Scholar]
- Bennett J. Chloroplast phosphoproteins. Phosphorylation of polypeptides of the light-harvesting chlorophyll protein complex. Eur J Biochem. 1979 Aug 15;99(1):133–137. doi: 10.1111/j.1432-1033.1979.tb13239.x. [DOI] [PubMed] [Google Scholar]
- Bennett J. Chloroplast phosphoproteins. The protein kinase of thylakoid membranes is light-dependent. FEBS Lett. 1979 Jul 15;103(2):342–344. doi: 10.1016/0014-5793(79)81358-7. [DOI] [PubMed] [Google Scholar]
- Bennett J., Steinback K. E., Arntzen C. J. Chloroplast phosphoproteins: regulation of excitation energy transfer by phosphorylation of thylakoid membrane polypeptides. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5253–5257. doi: 10.1073/pnas.77.9.5253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bennoun P. Correlation between states I and II in algae and the effect of magnesium on chloroplasts. Biochim Biophys Acta. 1974 Nov 19;368(2):141–147. doi: 10.1016/0005-2728(74)90144-3. [DOI] [PubMed] [Google Scholar]
- Bennoun P. Evidence for a respiratory chain in the chloroplast. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4352–4356. doi: 10.1073/pnas.79.14.4352. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bonaventura C., Myers J. Fluorescence and oxygen evolution from Chlorella pyrenoidosa. Biochim Biophys Acta. 1969;189(3):366–383. doi: 10.1016/0005-2728(69)90168-6. [DOI] [PubMed] [Google Scholar]
- Chua N. H., Bennoun P. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175–2179. doi: 10.1073/pnas.72.6.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Delepelaire P., Chua N. H. Electrophoretic purification of chlorophyll a/b-protein complexes from Chlamydomonas reinhardtii and spinach and analysis of their polypeptide compositions. J Biol Chem. 1981 Sep 10;256(17):9300–9307. [PubMed] [Google Scholar]
- Diner B., Mauzerall D. Feedback controlling oxygen production in a cross-reaction between two photosystems in photosynthesis. Biochim Biophys Acta. 1973 May 30;305(2):329–352. doi: 10.1016/0005-2728(73)90180-1. [DOI] [PubMed] [Google Scholar]
- Owens G. C., Ohad I. Phosphorylation of chlamydomonas reinhardi chloroplast membrane proteins in vivo and in vitro. J Cell Biol. 1982 Jun;93(3):712–718. doi: 10.1083/jcb.93.3.712. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steinback K. E., Bose S., Kyle D. J. Phosphorylation of the light-harvesting chlorophyll-protein regulates excitation energy distribution between photosystem II and photosystem I. Arch Biochem Biophys. 1982 Jun;216(1):356–361. doi: 10.1016/0003-9861(82)90221-1. [DOI] [PubMed] [Google Scholar]
- Wollman F. A., Diner B. A. Cation control of fluorescence emission, light scatter, and membrane stacking in pigment mutants of Chlamydomonas reinhardi. Arch Biochem Biophys. 1980 May;201(2):646–659. doi: 10.1016/0003-9861(80)90555-x. [DOI] [PubMed] [Google Scholar]