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. 1988 Aug;170(8):3543–3546. doi: 10.1128/jb.170.8.3543-3546.1988

In vitro transcription from the late promoter of bacteriophage P4.

J Keener 1, E C Dale 1, S Kustu 1, R Calendar 1
PMCID: PMC211326  PMID: 3403508

Abstract

The late genes of satellite bacteriophage P4 are cotranscribed from a single promoter which shares little homology with known classes of Escherichia coli promoters (E. Dale, G. Christie, and R. Calendar, J. Mol. Biol. 192:793-803, 1986). In a coupled transcription-translation system, the P4 late gene promoter was activated by either the delta protein of P4 or the ogr protein of helper phage P2 in the absence of any other phage-encoded factor. delta-dependent transcription was inhibited by antibodies to the sigma 70 subunit of E. coli RNA polymerase but was restored by purified sigma 70, indicating that activation of transcription by the delta protein of P4 is dependent on the sigma 70 holoenzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Artz S. W., Broach J. R. Histidine regulation in Salmonella typhimurium: an activator attenuator model of gene regulation. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3453–3457. doi: 10.1073/pnas.72.9.3453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birkeland N. K., Lindquist B. H. Coliphage P2 late control gene ogr. DNA sequence and product identification. J Mol Biol. 1986 Apr 5;188(3):487–490. doi: 10.1016/0022-2836(86)90170-1. [DOI] [PubMed] [Google Scholar]
  3. Casadaban M. J., Chou J., Cohen S. N. In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of exogenous proteins: Escherichia coli plasmid vectors for the detection and cloning of translational initiation signals. J Bacteriol. 1980 Aug;143(2):971–980. doi: 10.1128/jb.143.2.971-980.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christie G. E., Calendar R. Bacteriophage P2 late promoters. II. Comparison of the four late promoter sequences. J Mol Biol. 1985 Feb 5;181(3):373–382. doi: 10.1016/0022-2836(85)90226-8. [DOI] [PubMed] [Google Scholar]
  5. Christie G. E., Haggård-Ljungquist E., Feiwell R., Calendar R. Regulation of bacteriophage P2 late-gene expression: the ogr gene. Proc Natl Acad Sci U S A. 1986 May;83(10):3238–3242. doi: 10.1073/pnas.83.10.3238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dale E. C., Christie G. E., Calendar R. Organization and expression of the satellite bacteriophage P4 late gene cluster. J Mol Biol. 1986 Dec 20;192(4):793–803. doi: 10.1016/0022-2836(86)90029-x. [DOI] [PubMed] [Google Scholar]
  7. Fujiki H., Palm P., Zillig W., Calendar R., Sunshine M. Identification of a mutation within the structural gene for the a subunit of DNA-dependent RNA polymerase of E. coli. Mol Gen Genet. 1976 Apr 23;145(1):19–22. doi: 10.1007/BF00331552. [DOI] [PubMed] [Google Scholar]
  8. Harris J. D., Calendar R. Transcription map of satellite coliphage P4. Virology. 1978 Apr;85(2):343–358. doi: 10.1016/0042-6822(78)90443-9. [DOI] [PubMed] [Google Scholar]
  9. Helmann J. D., Chamberlin M. J. Structure and function of bacterial sigma factors. Annu Rev Biochem. 1988;57:839–872. doi: 10.1146/annurev.bi.57.070188.004203. [DOI] [PubMed] [Google Scholar]
  10. Hirschman J., Wong P. K., Sei K., Keener J., Kustu S. Products of nitrogen regulatory genes ntrA and ntrC of enteric bacteria activate glnA transcription in vitro: evidence that the ntrA product is a sigma factor. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7525–7529. doi: 10.1073/pnas.82.22.7525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kahn M., Ow D., Sauer B., Rabinowitz A., Calendar R. Genetic analysis of bacteriophage P4 using P4-plasmid ColE1 hybrids. Mol Gen Genet. 1980 Feb;177(3):399–412. doi: 10.1007/BF00271478. [DOI] [PubMed] [Google Scholar]
  12. Kalionis B., Pritchard M., Egan J. B. Control of gene expression in the P2-related temperate coliphages. IV. Concerning the late control gene and control of its transcription. J Mol Biol. 1986 Sep 20;191(2):211–220. doi: 10.1016/0022-2836(86)90258-5. [DOI] [PubMed] [Google Scholar]
  13. Lin C. S. Nucleotide sequence of the essential region of bacteriophage P4. Nucleic Acids Res. 1984 Nov 26;12(22):8667–8684. doi: 10.1093/nar/12.22.8667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lindqvist B. H. Expression of phage transcription in P2 lysogens infected with helper-dependent coliphage P4. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2752–2755. doi: 10.1073/pnas.71.7.2752. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Remaut E., Stanssens P., Fiers W. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene. 1981 Oct;15(1):81–93. doi: 10.1016/0378-1119(81)90106-2. [DOI] [PubMed] [Google Scholar]
  17. Shore D., Dehò G., Tsipis J., Goldstein R. Determination of capsid size by satellite bacteriophage P4. Proc Natl Acad Sci U S A. 1978 Jan;75(1):400–404. doi: 10.1073/pnas.75.1.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Simons R. W., Hoopes B. C., McClure W. R., Kleckner N. Three promoters near the termini of IS10: pIN, pOUT, and pIII. Cell. 1983 Sep;34(2):673–682. doi: 10.1016/0092-8674(83)90400-2. [DOI] [PubMed] [Google Scholar]
  19. Six E. W., Lindqvist B. H. Multiplication of bacteriophage P4 in the absence of replication of the DNA of its helper. Virology. 1971 Jan;43(1):8–15. doi: 10.1016/0042-6822(71)90219-4. [DOI] [PubMed] [Google Scholar]
  20. Six E. W. The helper dependence of satellite bacteriophage P4: which gene functions of bacteriophage P2 are needed by P4? Virology. 1975 Sep;67(1):249–263. doi: 10.1016/0042-6822(75)90422-5. [DOI] [PubMed] [Google Scholar]
  21. Souza L., Calendar R., Six E. W., Lindqvist B. H. A transactivation mutant of satellite phage P4. Virology. 1977 Aug;81(1):81–90. doi: 10.1016/0042-6822(77)90060-5. [DOI] [PubMed] [Google Scholar]
  22. Sunshine M. G., Sauer B. A bacterial mutation blocking P2 phage late gene expression. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2770–2774. doi: 10.1073/pnas.72.7.2770. [DOI] [PMC free article] [PubMed] [Google Scholar]

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