Abstract
The motility of individual, aggregation-competent amebae of Dictyostelium has been analyzed at different concentrations of cAMP under both nongradient and gradient conditions. The following is demonstrated: (a) concentrations of cAMP greater than 10(-8) M inhibit motility in a concentration-dependent fashion, decrease the frequency but not the degree of turning, and cause rounding in cell shape; (b) no concentration of cAMP stimulates motility, or positive chemokinesis; (c) concentrations of cAMP that stimulate a maximal chemotactic response do not affect motility and concentrations of cAMP that maximally inhibit motility do not stimulate chemotaxis under gradient conditions; and (d) the concentrations of cAMP that inhibit motility are identical under gradient and nongradient conditions.
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- Alcantara F., Monk M. Signal propagation during aggregation in the slime mould Dictyostelium discoideum. J Gen Microbiol. 1974 Dec;85(2):321–334. doi: 10.1099/00221287-85-2-321. [DOI] [PubMed] [Google Scholar]
- Benya P. D., Shaffer J. D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell. 1982 Aug;30(1):215–224. doi: 10.1016/0092-8674(82)90027-7. [DOI] [PubMed] [Google Scholar]
- Bonner J. T. Induction of stalk cell differentiation by cyclic AMP in the cellular slime mold Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1970 Jan;65(1):110–113. doi: 10.1073/pnas.65.1.110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Finney R., Varnum B., Soll D. R. "Erasure" in Dictyostelium: a dedifferentiation involving the programmed loss of chemotactic functions. Dev Biol. 1979 Dec;73(2):290–303. doi: 10.1016/0012-1606(79)90068-x. [DOI] [PubMed] [Google Scholar]
- Futrelle R. P., Traut J., McKee W. G. Cell behavior in Dictyostelium discoideum: preaggregation response to localized cyclic AMP pulses. J Cell Biol. 1982 Mar;92(3):807–821. doi: 10.1083/jcb.92.3.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garrod D. R. The cellular basis of movement of the migrating grex of the slime mould Dictyostelium discoideum: chemotactic and reaggregation behaviour of grex cells. J Embryol Exp Morphol. 1974 Aug;32(1):57–68. [PubMed] [Google Scholar]
- Gerisch G., Malchow D. Cyclic AMP receptors and the control of cell aggregation in Dictyostelium. Adv Cyclic Nucleotide Res. 1976;7:49–68. [PubMed] [Google Scholar]
- Kay R. R., Garrod D., Tilly R. Requirement for cell differentiation in Dictyostelium discoideum. Nature. 1978 Jan 5;271(5640):58–60. doi: 10.1038/271058a0. [DOI] [PubMed] [Google Scholar]
- Konijn T. M., Van De Meene J. G., Bonner J. T., Barkley D. S. The acrasin activity of adenosine-3',5'-cyclic phosphate. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1152–1154. doi: 10.1073/pnas.58.3.1152. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landfear S. M., Lodish H. F. A role for cyclic AMP in expression of developmentally regulated genes in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1044–1048. doi: 10.1073/pnas.77.2.1044. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loomis W. F., Sussman M. Commitment to the synthesis of a specific enzyme during cellular slime mold development. J Mol Biol. 1966 Dec 28;22(2):401–404. doi: 10.1016/0022-2836(66)90148-3. [DOI] [PubMed] [Google Scholar]
- Mato J. M., Konijn T. M. Chemotaxis and binding of cyclic AMP in cellular slime molds. Biochim Biophys Acta. 1975 Apr 7;385(2):173–179. doi: 10.1016/0304-4165(75)90345-1. [DOI] [PubMed] [Google Scholar]
- Newell P. C., Telser A., Sussman M. Alternative developmental pathways determined by environmental conditions in the cellular slime mold Dictyostelium discoideum. J Bacteriol. 1969 Nov;100(2):763–768. doi: 10.1128/jb.100.2.763-768.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryter A., Klein C., Brachet P. Dictyostelium discoideum surface changes elicited by high concentrations of cAMP. Exp Cell Res. 1979 Mar 15;119(2):373–380. doi: 10.1016/0014-4827(79)90366-5. [DOI] [PubMed] [Google Scholar]
- Soll D. R., Waddell D. R. Morphogenesis in the slime mold Dictyostelium discoideum. 1. The accumulation and erasure of "morphogenetic information". Dev Biol. 1975 Dec;47(2):292–302. doi: 10.1016/0012-1606(75)90283-3. [DOI] [PubMed] [Google Scholar]
- Soll D. R., Yarger J., Mirick M. Stationary phase and the cell cycle of Dictyostelium discoideum in liquid nutrient medium. J Cell Sci. 1976 May;20(3):513–523. doi: 10.1242/jcs.20.3.513. [DOI] [PubMed] [Google Scholar]
- Spiegelman B. M., Green H. Control of specific protein biosynthesis during the adipose conversion of 3T3 cells. J Biol Chem. 1980 Sep 25;255(18):8811–8818. [PubMed] [Google Scholar]
- Swanson J. A., Taylor D. L. Local and spatially coordinated movements in Dictyostelium discoideum amoebae during chemotaxis. Cell. 1982 Feb;28(2):225–232. doi: 10.1016/0092-8674(82)90340-3. [DOI] [PubMed] [Google Scholar]
- Town C. D., Gross J. D., Kay R. R. Cell differentiation without morphogenesis in Dictyostelium discoideum. Nature. 1976 Aug 19;262(5570):717–719. doi: 10.1038/262717a0. [DOI] [PubMed] [Google Scholar]
- Varnum B., Soll D. R. Chemoresponsiveness to cAMP and folic acid during growth, development, and dedifferentiation in Dictyostelium discoideum. Differentiation. 1981;18(3):151–160. doi: 10.1111/j.1432-0436.1981.tb01116.x. [DOI] [PubMed] [Google Scholar]
- Zigmond S. H. Mechanisms of sensing chemical gradients by polymorphonuclear leukocytes. Nature. 1974 May 31;249(456):450–452. doi: 10.1038/249450a0. [DOI] [PubMed] [Google Scholar]