Abstract
[3H]Serotonin is accumulated by a specific set of amacrine cells in the rabbit retina. These cells also accumulate the neurotoxin, 5,7- dihydroxytryptamine, and show signs of necrosis within 4 h of in vivo exposure to the drug. Biochemical analysis of [3H]serotonin uptake reveal a sodium- and temperature-dependent, high affinity uptake system with a Km of 0.94 microM and Vmax of 1.08 pmol/mg protein/min. [3H]Tryptophan is also accumulated in rabbit retinal homogenates by a high affinity process. Accumulated [3H]serotonin is released in response to potassium-induced depolarization of intact, isolated retinas. In vitro binding studies of rabbit retinal homogenate membranes demonstrate specific sets of binding sites with characteristics of the postsynaptic serotonin receptor. These data strongly suggest that rabbit retina contains virtually all of the molecular components required for a functional serotonergic neurotransmitter system. The only significant difference between the serotonin system in rabbit retina and that in the well-established serotonin transmitter systems in nonmammalin retinas and in brains of most species is the relatively low concentration of endogenous serotonin in rabbit retinas, as demonstrated by high-performance liquid chromatography, histofluorescence, or immunocytochemistry.
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Selected References
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- Ames A., 3rd, Pollen D. A. Neurotransmission in central nervous tissue: a study of isolated rabbit retina. J Neurophysiol. 1969 May;32(3):424–442. doi: 10.1152/jn.1969.32.3.424. [DOI] [PubMed] [Google Scholar]
- Baumgarten H. G., Björklund A., Lachenmayer L., Nobin A. Evaluation of the effects of 5,7-dihydroxytryptamine on serotonin and catecholamine neurons in the rat CNS. Acta Physiol Scand Suppl. 1973;391:1–19. [PubMed] [Google Scholar]
- Ehinger B., Florén I. Chemical removal of indoleamine accumulating terminals in rabbit and goldfish retina. Exp Eye Res. 1978 Mar;26(3):321–328. doi: 10.1016/0014-4835(78)90078-7. [DOI] [PubMed] [Google Scholar]
- Florén I. Arguments against 5-hydroxytryptamine as neurotransmitter in the rabbit retina. J Neural Transm. 1979;46(1):1–15. doi: 10.1007/BF01243425. [DOI] [PubMed] [Google Scholar]
- Florén I., Hansson H. C. Investigations into whether 5-hydroxytryptamine is a neurotransmitter in the retina of rabbit and chicken. Invest Ophthalmol Vis Sci. 1980 Feb;19(2):117–125. [PubMed] [Google Scholar]
- Hampton C. K., Garcia C., Redburn D. A. Localization of kainic acid-sensitive cells in mammalian retina. J Neurosci Res. 1981;6(1):99–111. doi: 10.1002/jnr.490060110. [DOI] [PubMed] [Google Scholar]
- Osborne N. N. Binding of [3H]serotonin to membranes of the bovine retina. Exp Eye Res. 1981 Oct;33(4):371–380. doi: 10.1016/s0014-4835(81)80089-9. [DOI] [PubMed] [Google Scholar]
- Osborne N. N. In vitro experiments on the metabolism, uptake and release of 5-hydroxytryptamine in bovine retina. Brain Res. 1980 Feb 24;184(2):283–297. doi: 10.1016/0006-8993(80)90799-4. [DOI] [PubMed] [Google Scholar]
- Osborne N. N., Nesselhut T., Nicholas D. A., Patel S., Cuello A. C. Serotonin-containing neurones in vertebrate retinas. J Neurochem. 1982 Dec;39(6):1519–1528. doi: 10.1111/j.1471-4159.1982.tb07984.x. [DOI] [PubMed] [Google Scholar]
- Osborne N. N. Uptake, localization and release of serotonin in the chick retina. J Physiol. 1982 Oct;331:469–479. doi: 10.1113/jphysiol.1982.sp014384. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PATTERSON M. S., GREENE R. C. MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS. Anal Chem. 1965 Jun;37:854–857. doi: 10.1021/ac60226a017. [DOI] [PubMed] [Google Scholar]
- Parkinson D., Rando R. R. Evidence for a neurotransmitter role for 5-hydroxytryptamine in chick retina. J Neurosci. 1981 Nov;1(11):1211–1217. doi: 10.1523/JNEUROSCI.01-11-01211.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peroutka S. J., Snyder S. H. Two distinct serotonin receptors: regional variations in receptor binding in mammalian brain. Brain Res. 1981 Mar 16;208(2):339–347. doi: 10.1016/0006-8993(81)90562-x. [DOI] [PubMed] [Google Scholar]
- Redburn D. A. Uptake and release of [14C]GABA from rabbit retina synaptosomes. Exp Eye Res. 1977 Sep;25(3):265–275. doi: 10.1016/0014-4835(77)90093-8. [DOI] [PubMed] [Google Scholar]
- Torre J. C., Surgeon J. W. A methodological approach to rapid and sensitive monoamine histofluorescence using a modified glyoxylic acid technique: the SPG method. Histochemistry. 1976 Oct 22;49(2):81–93. doi: 10.1007/BF00495672. [DOI] [PubMed] [Google Scholar]