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. 1985 Jan 1;100(1):322–326. doi: 10.1083/jcb.100.1.322

Bidirectional organelle transport can occur in cell processes that contain single microtubules

PMCID: PMC2113484  PMID: 3965478

Abstract

Intracellular organelle transport was studied in a new model system, the giant freshwater ameba Reticulomyxa. The ameba extends a large reticulate network of cytoplasmic strands in which various phase-dense organelles can be seen to move at a rate of up to 25 microns/s. This combined light and high voltage electron microscopic study shows that organelles move bidirectionally in even the finest network strands that contain only a single microtubule. In terms of microtubule-associated intracellular transport, this observation defines a minimum set of conditions necessary for such movement. The implications of this finding for possible models of force generation are discussed.

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Selected References

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  1. Allen R. D., Travis J. L., Hayden J. H., Allen N. S., Breuer A. C., Lewis L. J. Cytoplasmic transport: moving ultrastructural elements common to many cell types revealed by video-enhanced microscopy. Cold Spring Harb Symp Quant Biol. 1982;46(Pt 1):85–87. doi: 10.1101/sqb.1982.046.01.012. [DOI] [PubMed] [Google Scholar]
  2. Burton P. R., Paige J. L. Polarity of axoplasmic microtubules in the olfactory nerve of the frog. Proc Natl Acad Sci U S A. 1981 May;78(5):3269–3273. doi: 10.1073/pnas.78.5.3269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Euteneuer U., McIntosh J. R. Polarity of some motility-related microtubules. Proc Natl Acad Sci U S A. 1981 Jan;78(1):372–376. doi: 10.1073/pnas.78.1.372. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Freed J. J., Lebowitz M. M. The association of a class of saltatory movements with microtubules in cultured cells. J Cell Biol. 1970 May;45(2):334–354. doi: 10.1083/jcb.45.2.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grafstein B., Forman D. S. Intracellular transport in neurons. Physiol Rev. 1980 Oct;60(4):1167–1283. doi: 10.1152/physrev.1980.60.4.1167. [DOI] [PubMed] [Google Scholar]
  6. Hayden J. H., Allen R. D., Goldman R. D. Cytoplasmic transport in keratocytes: direct visualization of particle translocation along microtubules. Cell Motil. 1983;3(1):1–19. doi: 10.1002/cm.970030102. [DOI] [PubMed] [Google Scholar]
  7. Heidemann S. R., Landers J. M., Hamborg M. A. Polarity orientation of axonal microtubules. J Cell Biol. 1981 Dec;91(3 Pt 1):661–665. doi: 10.1083/jcb.91.3.661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hisanaga S., Sakai H. Cytoplasmic dynein of the sea urchin egg. II. Purification, characterization and interactions with microtubules and Ca-calmodulin. J Biochem. 1983 Jan;93(1):87–98. doi: 10.1093/oxfordjournals.jbchem.a134182. [DOI] [PubMed] [Google Scholar]
  9. Johnson L. V., Walsh M. L., Chen L. B. Localization of mitochondria in living cells with rhodamine 123. Proc Natl Acad Sci U S A. 1980 Feb;77(2):990–994. doi: 10.1073/pnas.77.2.990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murphy D. B., Tilney L. G. The role of microtubules in the movement of pigment granules in teleost melanophores. J Cell Biol. 1974 Jun;61(3):757–779. doi: 10.1083/jcb.61.3.757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sabnis D. D., Jacobs W. P. Cytoplasmic streaming and microtubules in the coenocytic marine alga, Caulerpa prolifera. J Cell Sci. 1967 Dec;2(4):465–472. doi: 10.1242/jcs.2.4.465. [DOI] [PubMed] [Google Scholar]
  12. Schliwa M. Chromatophores: their use in understanding microtubule-dependent intracellular transport. Methods Cell Biol. 1982;25(Pt B):285–312. doi: 10.1016/s0091-679x(08)61430-2. [DOI] [PubMed] [Google Scholar]
  13. Schliwa M. Mechanisms of intracellular organelle transport. Cell Muscle Motil. 1984;5:1-82,403-6. doi: 10.1007/978-1-4684-4592-3_1. [DOI] [PubMed] [Google Scholar]
  14. Schliwa M., van Blerkom J. Structural interaction of cytoskeletal components. J Cell Biol. 1981 Jul;90(1):222–235. doi: 10.1083/jcb.90.1.222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith D. S., Järlfors U., Cameron B. F. Morphological evidence for the participation of microtubules in axonal transport. Ann N Y Acad Sci. 1975 Jun 30;253:472–506. doi: 10.1111/j.1749-6632.1975.tb19223.x. [DOI] [PubMed] [Google Scholar]
  16. Travis J. L., Allen R. D. Studies on the motility of the foraminifera. I. Ultrastructure of the reticulopodial network of Allogromia laticollaris (Arnold). J Cell Biol. 1981 Jul;90(1):211–221. doi: 10.1083/jcb.90.1.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Travis J. L., Kenealy J. F., Allen R. D. Studies on the motility of the foraminifera. II. The dynamic microtubular cytoskeleton of the reticulopodial network of Allogromia laticollaris. J Cell Biol. 1983 Dec;97(6):1668–1676. doi: 10.1083/jcb.97.6.1668. [DOI] [PMC free article] [PubMed] [Google Scholar]

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