Abstract
Human scleroderma serum 5051, which is known to recognize the amorphous pericentriolar microtubule organizing center material of a variety of vertebrate cells, was found to immunostain spindle poles of meristematic higher plants from pre-prophase to late anaphase. Subsequently, during cytokinesis, staining was redistributed around the reforming telophase nuclei, but was not evident in the cytokinetic phragmoplast. At the transition between telophase and interphase, before the typical cortical interphase microtubule array was established, short microtubules radiated from the nucleus and in such cells the material recognized by 5051 was located around the daughter nuclei and not the cortex. These observations have led us to propose that the perinuclear region, or the nuclear surface, may function as a nucleation center for both spindle and interphase microtubules in higher plant cells.
Full Text
The Full Text of this article is available as a PDF (2.0 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Calarco-Gillam P. D., Siebert M. C., Hubble R., Mitchison T., Kirschner M. Centrosome development in early mouse embryos as defined by an autoantibody against pericentriolar material. Cell. 1983 Dec;35(3 Pt 2):621–629. doi: 10.1016/0092-8674(83)90094-6. [DOI] [PubMed] [Google Scholar]
- De Mey J., Lambert A. M., Bajer A. S., Moeremans M., De Brabander M. Visualization of microtubules in interphase and mitotic plant cells of Haemanthus endosperm with the immuno-gold staining method. Proc Natl Acad Sci U S A. 1982 Mar;79(6):1898–1902. doi: 10.1073/pnas.79.6.1898. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Euteneuer U., McIntosh J. R. Polarity of midbody and phragmoplast microtubules. J Cell Biol. 1980 Nov;87(2 Pt 1):509–515. doi: 10.1083/jcb.87.2.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gould R. R., Borisy G. G. The pericentriolar material in Chinese hamster ovary cells nucleates microtubule formation. J Cell Biol. 1977 Jun;73(3):601–615. doi: 10.1083/jcb.73.3.601. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardham A. R., Gunning B. E. Interpolation of microtubules into cortical arrays during cell elongation and differentiation in roots of Azolla pinnata. J Cell Sci. 1979 Jun;37:411–442. doi: 10.1242/jcs.37.1.411. [DOI] [PubMed] [Google Scholar]
- Hepler P. K., Jackson W. T. Microtubules and early stages of cell-plate formation in the endosperm of Haemanthus katherinae Baker. J Cell Biol. 1968 Aug;38(2):437–446. doi: 10.1083/jcb.38.2.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilmartin J. V., Wright B., Milstein C. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J Cell Biol. 1982 Jun;93(3):576–582. doi: 10.1083/jcb.93.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lloyd C. W. Helical microtubular arrays in onion root hairs. Nature. 1983 Sep 22;305(5932):311–313. doi: 10.1038/305311a0. [DOI] [PubMed] [Google Scholar]
- Mazia D. Centrosomes and mitotic poles. Exp Cell Res. 1984 Jul;153(1):1–15. doi: 10.1016/0014-4827(84)90442-7. [DOI] [PubMed] [Google Scholar]
- Neumann D., Scharf K. D., Nover L. Heat shock induced changes of plant cell ultrastructure and autoradiographic localization of heat shock proteins. Eur J Cell Biol. 1984 Jul;34(2):254–264. [PubMed] [Google Scholar]
- Vandre D. D., Davis F. M., Rao P. N., Borisy G. G. Phosphoproteins are components of mitotic microtubule organizing centers. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4439–4443. doi: 10.1073/pnas.81.14.4439. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wick S. M., Duniec J. Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. I. Preprophase band development and concomitant appearance of nuclear envelope-associated tubulin. J Cell Biol. 1983 Jul;97(1):235–243. doi: 10.1083/jcb.97.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wick S. M., Seagull R. W., Osborn M., Weber K., Gunning B. E. Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J Cell Biol. 1981 Jun;89(3):685–690. doi: 10.1083/jcb.89.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]