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. 1986 Jul 1;103(1):33–39. doi: 10.1083/jcb.103.1.33

270K microtubule-associated protein cross-reacting with anti-MAP2 IgG in the crayfish peripheral nerve axon

PMCID: PMC2113782  PMID: 3722268

Abstract

MAPs (microtubule-associated proteins) were isolated from crayfish walking leg nerves. A major MAP was identified as a high molecular weight protein (270K). This protein co-migrated with mammalian MAP2, stimulated the polymerization of rat brain tubulin into microtubules, and was heat resistant. Rotary shadowing revealed that the 270K MAP is a long thin flexible structure. It formed cross-bridges of fine strands, linking microtubules with each other in vitro. These strands resemble the cross-bridges between microtubules observed in the crayfish axon permeabilized with saponin and quick-frozen, deep-etched. Antibodies against mammalian MAP2 cross-reacted with this crayfish MAP and stained the axoplasm of the walking leg nerves. Thus MAPs, especially the 270K MAP, appear to be a major component of the cross- linking strands between microtubules observed in the crayfish axon.

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Selected References

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