Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Sep;170(9):3864–3869. doi: 10.1128/jb.170.9.3864-3869.1988

Cloning of the fic-1 gene involved in cell filamentation induced by cyclic AMP and construction of a delta fic Escherichia coli strain.

M Kawamukai 1, H Matsuda 1, W Fujii 1, T Nishida 1, Y Izumoto 1, M Himeno 1, R Utsumi 1, T Komano 1
PMCID: PMC211382  PMID: 2842288

Abstract

PA3092 is an Escherichia coli mutant that forms filaments at 43 degrees C in the presence of cyclic AMP (cAMP). The mutation responsible for this phenotype is called fic-1. We cloned fic-1 from PA3092 by selection for the neighboring argD gene. The fic-1 gene product had a relative molecular mass of 21 kilodaltons by the maxicell method. A strain with the fic gene completely deleted was constructed by replacing fic with a kanamycin resistance gene. In one of the fic-deleted strains derived from PA3092, cAMP did not induce cell filamentation at 43 degrees C, but it did in the same strain harboring a plasmid containing the fic-1 gene. These results indicate that the fic-1 gene product is necessary for the induction of cell filamentation by cAMP but is dispensable to the cell. We also found that high levels of NaCl suppressed the cell filamentation induced by cAMP.

Full text

PDF
3864

Images in this article

3866Image
on p.3866
3866Image
on p.3866
3867Image
on p.3867

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiba H., Fujimoto S., Ozaki N. Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res. 1982 Feb 25;10(4):1345–1361. doi: 10.1093/nar/10.4.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aiba H., Nakamura T., Mitani H., Mori H. Mutations that alter the allosteric nature of cAMP receptor protein of Escherichia coli. EMBO J. 1985 Dec 1;4(12):3329–3332. doi: 10.1002/j.1460-2075.1985.tb04084.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Aiba H. Transcription of the Escherichia coli adenylate cyclase gene is negatively regulated by cAMP-cAMP receptor protein. J Biol Chem. 1985 Mar 10;260(5):3063–3070. [PubMed] [Google Scholar]
  4. Aono R., Yamasaki M., Tamura G. High and selective resistance to mecillinam in adenylate cyclase-deficient or cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli. J Bacteriol. 1979 Feb;137(2):839–845. doi: 10.1128/jb.137.2.839-845.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benner D., Müller N., Boos W. Temperature-sensitive catabolite activator protein in Escherichia coli BUG6. J Bacteriol. 1985 Jan;161(1):347–352. doi: 10.1128/jb.161.1.347-352.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cook W. R., Ringler N., Black B., Bernlohr R. W. Accumulation of cyclic GMP in filaments of Escherichia coli BUG6. J Bacteriol. 1983 Jul;155(1):69–73. doi: 10.1128/jb.155.1.69-73.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Garges S., Adhya S. Sites of allosteric shift in the structure of the cyclic AMP receptor protein. Cell. 1985 Jul;41(3):745–751. doi: 10.1016/s0092-8674(85)80055-6. [DOI] [PubMed] [Google Scholar]
  9. Jaffé A., D'Ari R., Norris V. SOS-independent coupling between DNA replication and cell division in Escherichia coli. J Bacteriol. 1986 Jan;165(1):66–71. doi: 10.1128/jb.165.1.66-71.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kato J., Suzuki H., Hirota Y. Dispensability of either penicillin-binding protein-1a or -1b involved in the essential process for cell elongation in Escherichia coli. Mol Gen Genet. 1985;200(2):272–277. doi: 10.1007/BF00425435. [DOI] [PubMed] [Google Scholar]
  11. Kawamukai M., Kishimoto J., Utsumi R., Himeno M., Komano T., Aiba H. Negative regulation of adenylate cyclase gene (cya) expression by cyclic AMP-cyclic AMP receptor protein in Escherichia coli: studies with cya-lac protein and operon fusion plasmids. J Bacteriol. 1985 Nov;164(2):872–877. doi: 10.1128/jb.164.2.872-877.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kumar S., Prakash N., Sharma V. K. Control of minicell producing cell division by cAMP-receptor protein complex in Escherichia coli. Mol Gen Genet. 1979 Nov;176(3):449–450. doi: 10.1007/BF00333110. [DOI] [PubMed] [Google Scholar]
  13. Kumar S. Properties of adenyl cyclase and cyclic adenosine 3',5'-monophosphate receptor protein-deficient mutants of Escherichia coli. J Bacteriol. 1976 Feb;125(2):545–555. doi: 10.1128/jb.125.2.545-555.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Reddy P., Peterkofsky A., McKenney K. Translational efficiency of the Escherichia coli adenylate cyclase gene: mutating the UUG initiation codon to GUG or AUG results in increased gene expression. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5656–5660. doi: 10.1073/pnas.82.17.5656. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Riley M., Glansdorff N. Cloning the Escherichia coli K-12 argD gene specifying acetylornithine delta-transaminase. Gene. 1983 Oct;24(2-3):335–339. doi: 10.1016/0378-1119(83)90095-1. [DOI] [PubMed] [Google Scholar]
  16. Russell R. R. Temperature-sensitive osmotic remedial mutants of Escherichia coli. J Bacteriol. 1972 Nov;112(2):661–665. doi: 10.1128/jb.112.2.661-665.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sancar A., Hack A. M., Rupp W. D. Simple method for identification of plasmid-coded proteins. J Bacteriol. 1979 Jan;137(1):692–693. doi: 10.1128/jb.137.1.692-693.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Utsumi R., Kawamukai M., Aiba H., Himeno M., Komano T. Expression of the adenylate cyclase gene during cell elongation in Escherichia coli K-12. J Bacteriol. 1986 Dec;168(3):1408–1414. doi: 10.1128/jb.168.3.1408-1414.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Utsumi R., Kawamukai M., Obata K., Morita J., Himeno M., Komano T. Identification of a membrane protein induced concurrently with cell filamentation by cyclic AMP in an Escherichia coli K-12 fic mutant. J Bacteriol. 1983 Jul;155(1):398–401. doi: 10.1128/jb.155.1.398-401.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Utsumi R., Nakamoto Y., Kawamukai M., Himeno M., Komano T. Involvement of cyclic AMP and its receptor protein in filamentation of an Escherichia coli fic mutant. J Bacteriol. 1982 Aug;151(2):807–812. doi: 10.1128/jb.151.2.807-812.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Utsumi R., Tanabe H., Nakamoto Y., Kawamukai M., Sakai H., Himeno M., Komano T., Hirota Y. Inhibitory effect of adenosine 3',5'-phosphate on cell division of Escherichia coli K-12 mutant derivatives. J Bacteriol. 1981 Sep;147(3):1105–1109. doi: 10.1128/jb.147.3.1105-1109.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  24. de Crombrugghe B., Busby S., Buc H. Cyclic AMP receptor protein: role in transcription activation. Science. 1984 May 25;224(4651):831–838. doi: 10.1126/science.6372090. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES