Abstract
A protein (Mr = 63,000) from calf serum that promotes the pigmentation of cultured chick neural crest and mouse melanoma cells has been partially isolated and characterized in this study. The stimulation of melanin synthesis in cultured cells was used to follow its activity during purification. The pigment-promoting factor was isolated by sequential column chromatography on dye-agarose matrices followed by hydroxyapatite and high pressure molecular sieve chromatography. The factor was found to stimulate melanin biosynthesis at 2-4 micrograms/ml and was specific for melanin-producing cells and their precursors. Antibodies raised in rabbits against the factor inhibited its pigment- promoting activity as well as that of whole calf serum. Enzyme-linked immunoadsorbent assays demonstrated that calf and bovine sera contain molecules that cross-react with the pigment-promoting factor. Horse, human, rat, and chicken sera, which lack the biological activity, also lacked immunological cross-reactivity. Extracts of certain tissues, particularly the submaxillary gland, were observed to be rich sources of pigment-promoting activity.
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- Bee J., Thorogood P. The role of tissue interactions in the skeletogenic differentiation of avian neural crest cells. Dev Biol. 1980 Jul;78(1):47–62. doi: 10.1016/0012-1606(80)90317-6. [DOI] [PubMed] [Google Scholar]
- Derby M. A., Newgreen D. F. Differentiation of avian neural crest cells in vitro: absence of a developmental bias toward melanogenesis. Cell Tissue Res. 1982;225(2):365–378. doi: 10.1007/BF00214689. [DOI] [PubMed] [Google Scholar]
- Greenberg J. H., Schrier B. K. Development of choline acetyltransferase activity in chick cranial neural crest cells in culture. Dev Biol. 1977 Nov;61(1):86–93. doi: 10.1016/0012-1606(77)90344-x. [DOI] [PubMed] [Google Scholar]
- Hall B. K. Tissue interactions and the initiation of osteogenesis and chondrogenesis in the neural crest-derived mandibular skeleton of the embryonic mouse as seen in isolated murine tissues and in recombinations of murine and avian tissues. J Embryol Exp Morphol. 1980 Aug;58:251–264. [PubMed] [Google Scholar]
- Kreider J. W., Schmoyer M. E. Spontaneous maturation and differentiation of B16 melanoma cells in culture. J Natl Cancer Inst. 1975 Sep;55(3):641–647. doi: 10.1093/jnci/55.3.641. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Laskin J. D., Mufson R. A., Weinstein I. B., Engelhardt D. L. Identification of a distinct phase during melanogenesis that is sensitive to extracellular pH and ionic strength. J Cell Physiol. 1980 Jun;103(3):467–474. doi: 10.1002/jcp.1041030312. [DOI] [PubMed] [Google Scholar]
- Laskin J. D., Piccinini L., Engelhardt D. L., Weinstein I. B. Control of melanin synthesis and secretion by B16/C3 melanoma cells. J Cell Physiol. 1982 Dec;113(3):481–486. doi: 10.1002/jcp.1041130318. [DOI] [PubMed] [Google Scholar]
- Mufson R. A., Fisher P. B., Weinstein I. B. Effect of phorbol ester tumor promoters on the expression of melanogenesis in B-16 melanoma cells. Cancer Res. 1979 Oct;39(10):3915–3919. [PubMed] [Google Scholar]
- Newsome D. A. In vitro stimulation of cartilage in embryonic chick neural crest cells by products of rentinal pigmented epithelium. Dev Biol. 1976 Apr;49(2):496–507. doi: 10.1016/0012-1606(76)90191-3. [DOI] [PubMed] [Google Scholar]
- Rennard S. I., Berg R., Martin G. R., Foidart J. M., Robey P. G. Enzyme-linked immunoassay (ELISA) for connective tissue components. Anal Biochem. 1980 May 1;104(1):205–214. doi: 10.1016/0003-2697(80)90300-0. [DOI] [PubMed] [Google Scholar]
- Weston J. A. The migration and differentiation of neural crest cells. Adv Morphog. 1970;8:41–114. doi: 10.1016/b978-0-12-028608-9.50006-5. [DOI] [PubMed] [Google Scholar]
- Whittaker J. R. Biosynthesis of a thouracil pheomelanin in embryonic pigment cells exposed to thouracil. J Biol Chem. 1971 Oct 25;246(20):6217–6226. [PubMed] [Google Scholar]