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. 1985 Dec 1;101(6):2233–2238. doi: 10.1083/jcb.101.6.2233

Endothelial plasmalemmal vesicles have a characteristic striped bipolar surface structure

PMCID: PMC2113993  PMID: 4066756

Abstract

Capillary endothelial cells have a large population of small (65-80 nm diameter in transmission electron microscopy) vesicles of which a large fraction is associated with the plasmalemma of the luminal and abluminal side. We studied the fine structure and distribution of these plasmalemmal vesicles by high resolution scanning electron microscopy in cultured endothelial cells obtained from bovine adrenal cortical capillaries. Cell monolayers were covered with polylysine-coated silicon chips, split in high potassium buffer, fixed in aldehyde mixtures, and then treated with OsO4 and thiocarbohydrazide. After critical point drying, the specimens were coated with a thin (less than 2 nm) continuous film of chromium. On the cytoplasmic aspect of the dorsal plasmalemmal fragments seen in such specimens, plasmalemmal vesicles appear as uniform vesicular protrusions approximately 70-90 nm in diameter, preferentially concentrated in distinct large fields in which they occur primarily as single units. Individual plasmalemmal vesicles exhibit a striped surface fine structure which consists of ridges approximately 10 nm in diameter, separated by furrows and oriented as meridians, often ending at two poles on opposite sides of the vesicles in a plane parallel to the plasmalemma. This striped surface structure is clearly distinct from the cage structure of coated pits found, at low surface density, on the same specimens. The cytoplasmic aspect of the plasmalemma proper is covered by a fibrillar infrastructure which does not extend over plasmalemmal vesicles but on which the latter appear to be anchored by fine filaments.

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Selected References

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  1. Aggeler J., Takemura R., Werb Z. High-resolution three-dimensional views of membrane-associated clathrin and cytoskeleton in critical-point-dried macrophages. J Cell Biol. 1983 Nov;97(5 Pt 1):1452–1458. doi: 10.1083/jcb.97.5.1452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bruns R. R., Palade G. E. Studies on blood capillaries. II. Transport of ferritin molecules across the wall of muscle capillaries. J Cell Biol. 1968 May;37(2):277–299. doi: 10.1083/jcb.37.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bundgaard M., Frøkjaer-Jensen J., Crone C. Endothelial plasmalemmal vesicles as elements in a system of branching invaginations from the cell surface. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6439–6442. doi: 10.1073/pnas.76.12.6439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bundgaard M., Hagman P., Crone C. The three-dimensional organization of plasmalemmal vesicular profiles in the endothelium of rat heart capillaries. Microvasc Res. 1983 May;25(3):358–368. doi: 10.1016/0026-2862(83)90025-0. [DOI] [PubMed] [Google Scholar]
  5. Crowther R. A., Finch J. T., Pearse B. M. On the structure of coated vesicles. J Mol Biol. 1976 Jun 5;103(4):785–798. doi: 10.1016/0022-2836(76)90209-6. [DOI] [PubMed] [Google Scholar]
  6. Frøkjaer-Jensen J. Three-dimensional organization of plasmalemmal vesicles in endothelial cells. An analysis by serial sectioning of frog mesenteric capillaries. J Ultrastruct Res. 1980 Oct;73(1):9–20. doi: 10.1016/0022-5320(80)90111-2. [DOI] [PubMed] [Google Scholar]
  7. Heuser J. Three-dimensional visualization of coated vesicle formation in fibroblasts. J Cell Biol. 1980 Mar;84(3):560–583. doi: 10.1083/jcb.84.3.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirokawa N., Heuser J. E. Internal and external differentiations of the postsynaptic membrane at the neuromuscular junction. J Neurocytol. 1982 Jun;11(3):487–510. doi: 10.1007/BF01257990. [DOI] [PubMed] [Google Scholar]
  9. Joyce N. C., Haire M. F., Palade G. E. Contractile proteins in pericytes. II. Immunocytochemical evidence for the presence of two isomyosins in graded concentrations. J Cell Biol. 1985 May;100(5):1387–1395. doi: 10.1083/jcb.100.5.1387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mazia D., Schatten G., Sale W. Adhesion of cells to surfaces coated with polylysine. Applications to electron microscopy. J Cell Biol. 1975 Jul;66(1):198–200. doi: 10.1083/jcb.66.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Peters K. R. Conditions required for high quality high magnification images in secondary electron-I scanning electron microscopy. Scan Electron Microsc. 1982;(Pt 4):1359–1372. [PubMed] [Google Scholar]
  12. Pratt B. M., Harris A. S., Morrow J. S., Madri J. A. Mechanisms of cytoskeletal regulation. Modulation of aortic endothelial cell spectrin by the extracellular matrix. Am J Pathol. 1984 Dec;117(3):349–354. [PMC free article] [PubMed] [Google Scholar]
  13. Prescott L., Brightman M. W. The sarcolemma of Aplysia smooth muscle in freeze-fracture preparations. Tissue Cell. 1976;8(2):248–258. [PubMed] [Google Scholar]
  14. Renkin E. M. Relation of capillary morphology to transport of fluid and large molecules: a review. Acta Physiol Scand Suppl. 1979;463:81–91. [PubMed] [Google Scholar]
  15. Simionescu M., Simionescu N., Palade G. E. Differentiated microdomains on the luminal surface of capillary endothelium: distribution of lectin receptors. J Cell Biol. 1982 Aug;94(2):406–413. doi: 10.1083/jcb.94.2.406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Simionescu M., Simionescu N., Santoro F., Palade G. E. Differentiated microdomains of the luminal plasmalemma of murine muscle capillaries: segmental variations in young and old animals. J Cell Biol. 1985 May;100(5):1396–1407. doi: 10.1083/jcb.100.5.1396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Simionescu N. Cellular aspects of transcapillary exchange. Physiol Rev. 1983 Oct;63(4):1536–1579. doi: 10.1152/physrev.1983.63.4.1536. [DOI] [PubMed] [Google Scholar]
  18. Simionescu N., Siminoescu M., Palade G. E. Permeability of muscle capillaries to small heme-peptides. Evidence for the existence of patent transendothelial channels. J Cell Biol. 1975 Mar;64(3):586–607. doi: 10.1083/jcb.64.3.586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Simionescu N., Simionescu M., Palade G. E. Differentiated microdomains on the luminal surface of the capillary endothelium. I. Preferential distribution of anionic sites. J Cell Biol. 1981 Sep;90(3):605–613. doi: 10.1083/jcb.90.3.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wagner R. C., Casley-Smith J. R. Review. Endothelial vesicles. Microvasc Res. 1981 May;21(3):267–298. doi: 10.1016/0026-2862(81)90011-x. [DOI] [PubMed] [Google Scholar]
  21. Wagner R. C., Robinson C. S. High-voltage electron microscopy of capillary endothelial vesicles. Microvasc Res. 1984 Sep;28(2):197–205. doi: 10.1016/0026-2862(84)90017-7. [DOI] [PubMed] [Google Scholar]

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