Skip to main content
PMC home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1986 Feb 1;102(2):492–499. doi: 10.1083/jcb.102.2.492

Secretion of a cytoplasmic lectin from Xenopus laevis skin

PMCID: PMC2114084  PMID: 3511073

Abstract

The skin of Xenopus laevis contains a soluble beta-galactoside-binding lectin with a approximately 16,000-mol-wt subunit. It resembles similar lectins purified from a variety of tissues from other vertebrates, and differs from two other soluble X. laevis lectins from oocytes and serum that bind alpha-galactosides. The skin lectin is concentrated in the cytoplasm of granular gland and mucous gland cells, as demonstrated by immunohistochemistry with the electron microscope. Upon injection with epinephrine, there is massive secretion of the cytoplasmic lectin from the granular gland cells.

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barondes S. H., Haywood-Reid P. L. Externalization of an endogenous chicken muscle lectin with in vivo development. J Cell Biol. 1981 Nov;91(2 Pt 1):568–572. doi: 10.1083/jcb.91.2.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barondes S. H. Soluble lectins: a new class of extracellular proteins. Science. 1984 Mar 23;223(4642):1259–1264. doi: 10.1126/science.6367039. [DOI] [PubMed] [Google Scholar]
  3. Beyer E. C., Barondes S. H. Secretion of endogenous lectin by chicken intestinal goblet cells. J Cell Biol. 1982 Jan;92(1):28–33. doi: 10.1083/jcb.92.1.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beyer E. C., Tokuyasu K. T., Barondes S. H. Localization of an endogenous lectin in chicken liver, intestine, and pancreas. J Cell Biol. 1979 Aug;82(2):565–571. doi: 10.1083/jcb.82.2.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Beyer E. C., Zweig S. E., Barondes S. H. Two lactose binding lectins from chicken tissues. Purified lectin from intestine is different from those in liver and muscle. J Biol Chem. 1980 May 10;255(9):4236–4239. [PubMed] [Google Scholar]
  6. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  7. Briles E. B., Gregory W., Fletcher P., Kornfeld S. Vertebrate lectins, Comparison of properties of beta-galactoside-binding lectins from tissues of calf and chicken. J Cell Biol. 1979 Jun;81(3):528–537. doi: 10.1083/jcb.81.3.528. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cerra R. F., Gitt M. A., Barondes S. H. Three soluble rat beta-galactoside-binding lectins. J Biol Chem. 1985 Sep 5;260(19):10474–10477. [PubMed] [Google Scholar]
  9. Cerra R. F., Haywood-Reid P. L., Barondes S. H. Endogenous mammalian lectin localized extracellularly in lung elastic fibers. J Cell Biol. 1984 Apr;98(4):1580–1589. doi: 10.1083/jcb.98.4.1580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Childs R. A., Feizi T. beta-Galactoside-binding muscle lectins of man and monkey show antigenic cross-reactions with those of bovine origin. Biochem J. 1979 Dec 1;183(3):755–758. doi: 10.1042/bj1830755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Den H., Malinzak D. A. Isolation and properties of beta-D-galactoside-specific lectin from chick embryo thigh muscle. J Biol Chem. 1977 Aug 10;252(15):5444–5448. [PubMed] [Google Scholar]
  12. Dockray G. J., Hopkins C. R. Caerulein secretion by dermal glands in Xenopus laevis. J Cell Biol. 1975 Mar;64(3):724–733. doi: 10.1083/jcb.64.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gartner T. K., Ogilvie M. L. Isolation and characterization of three Ca2+-dependent beta-galactoside-specific lectins from snake venoms. Biochem J. 1984 Nov 15;224(1):301–307. doi: 10.1042/bj2240301. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Levi G., Teichberg V. I. Isolation and physicochemical characterization of electrolectin, a beta-D-galactoside binding lectin from the electric organ of Electrophorus electricus. J Biol Chem. 1981 Jun 10;256(11):5735–5740. [PubMed] [Google Scholar]
  15. Morrissey J. H. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem. 1981 Nov 1;117(2):307–310. doi: 10.1016/0003-2697(81)90783-1. [DOI] [PubMed] [Google Scholar]
  16. Nitta K., Takayanagi G., Terasaki Y., Kawauchi H. Studies on three kinds of lectins from Xenopus laevis skin. Experientia. 1984 Jul 15;40(7):712–713. doi: 10.1007/BF01949737. [DOI] [PubMed] [Google Scholar]
  17. Nowak T. P., Kobiler D., Roel L. E., Barondes S. H. Developmentally regulated lectin from embryonic chick pectoral muscle. Purification by affinity chromatography. J Biol Chem. 1977 Sep 10;252(17):6026–6030. [PubMed] [Google Scholar]
  18. Roberson M. M., Barondes S. H. Lectin from embryos and oocytes of Xenopus laevis. Purification and properties. J Biol Chem. 1982 Jul 10;257(13):7520–7524. [PubMed] [Google Scholar]
  19. Roberson M. M., Wolffe A. P., Tata J. R., Barondes S. H. Galactoside-binding serum lectin of Xenopus laevis. Estrogen-dependent hepatocyte synthesis and relationship to oocyte lectin. J Biol Chem. 1985 Sep 15;260(20):11027–11032. [PubMed] [Google Scholar]
  20. Whitney P., Maxwell S., Ryan U., Massaro D. Synthesis and binding of lactose-specific lectin by isolated lung cells. Am J Physiol. 1985 Mar;248(3 Pt 1):C258–C264. doi: 10.1152/ajpcell.1985.248.3.C258. [DOI] [PubMed] [Google Scholar]
  21. de Waard A., Hickman S., Kornfeld S. Isolation and properties of beta-galactoside binding lectins of calf heart and lung. J Biol Chem. 1976 Dec 10;251(23):7581–7587. [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES