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. 1986 Mar 1;102(3):711–715. doi: 10.1083/jcb.102.3.711

Localization of the human NCAM gene to band q23 of chromosome 11: the third gene coding for a cell interaction molecule mapped to the distal portion of the long arm of chromosome 11

PMCID: PMC2114109  PMID: 2869046

Abstract

cDNA clones containing sequences coding for the murine neural cell adhesion molecule (N-CAM) were used in Southern hybridizations on human genomic DNA and demonstrated approximately 90% homology between human and murine NCAM genes. In situ hybridization with one of these clones was performed on human metaphase chromosomes and allowed the localization of the human NCAM gene to band q23 of chromosome 11. The genes for two other cell surface molecules believed to be involved in cell-cell interactions, Thy-1 and the delta chain of the T3-T cell receptor complex, have recently been localized to the same region of chromosome 11 in man. Moreover, this region of the human chromosome 11 appears to be syntenic to a region of murine chromosome 9 that also contains the staggerer locus: staggerer mice show abnormal neurological features which may be related to abnormalities in the conversion of the embryonic to the adult forms of the N-CAM molecule.

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Selected References

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  1. Antonucci T. K., Von Deimling O. H., Rosenblum B. B., Skow L. C., Meisler M. H. Conserved linkage within a 4-cM region of mouse chromosome 9 and human chromosome 11. Genetics. 1984 Jul;107(3):463–475. doi: 10.1093/genetics/107.3.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beltz G. A., Jacobs K. A., Eickbush T. H., Cherbas P. T., Kafatos F. C. Isolation of multigene families and determination of homologies by filter hybridization methods. Methods Enzymol. 1983;100:266–285. doi: 10.1016/0076-6879(83)00061-0. [DOI] [PubMed] [Google Scholar]
  3. D'Eustachio P., Owens G. C., Edelman G. M., Cunningham B. A. Chromosomal location of the gene encoding the neural cell adhesion molecule (N-CAM) in the mouse. Proc Natl Acad Sci U S A. 1985 Nov;82(22):7631–7635. doi: 10.1073/pnas.82.22.7631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelman G. M., Chuong C. M. Embryonic to adult conversion of neural cell adhesion molecules in normal and staggerer mice. Proc Natl Acad Sci U S A. 1982 Nov;79(22):7036–7040. doi: 10.1073/pnas.79.22.7036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Edelman G. M., Hoffman S., Chuong C. M., Thiery J. P., Brackenbury R., Gallin W. J., Grumet M., Greenberg M. E., Hemperly J. J., Cohen C. Structure and modulation of neural cell adhesion molecules in early and late embryogenesis. Cold Spring Harb Symp Quant Biol. 1983;48(Pt 2):515–526. doi: 10.1101/sqb.1983.048.01.056. [DOI] [PubMed] [Google Scholar]
  6. Feinberg A. P., Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. doi: 10.1016/0003-2697(84)90381-6. [DOI] [PubMed] [Google Scholar]
  7. Finne J., Finne U., Deagostini-Bazin H., Goridis C. Occurrence of alpha 2-8 linked polysialosyl units in a neural cell adhesion molecule. Biochem Biophys Res Commun. 1983 Apr 29;112(2):482–487. doi: 10.1016/0006-291x(83)91490-0. [DOI] [PubMed] [Google Scholar]
  8. Gennarini G., Hirn M., Deagostini-Bazin H., Goridis C. Studies on the transmembrane disposition of the neural cell adhesion molecule N-CAM. The use of liposome-inserted radioiodinated N-CAM to study its transbilayer orientation. Eur J Biochem. 1984 Jul 2;142(1):65–73. doi: 10.1111/j.1432-1033.1984.tb08251.x. [DOI] [PubMed] [Google Scholar]
  9. Goridis C., Hirn M., Santoni M. J., Gennarini G., Deagostini-Bazin H., Jordan B. R., Kiefer M., Steinmetz M. Isolation of mouse N-CAM-related cDNA: detection and cloning using monoclonal antibodies. EMBO J. 1985 Mar;4(3):631–635. doi: 10.1002/j.1460-2075.1985.tb03676.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Grumet M., Rutishauser U., Edelman G. M. Neural cell adhesion molecule is on embryonic muscle cells and mediates adhesion to nerve cells in vitro. Nature. 1982 Feb 25;295(5851):693–695. doi: 10.1038/295693a0. [DOI] [PubMed] [Google Scholar]
  11. Hoffman S., Chuong C. M., Edelman G. M. Evolutionary conservation of key structures and binding functions of neural cell adhesion molecules. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6881–6885. doi: 10.1073/pnas.81.21.6881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lyles J. M., Norrild B., Bock E. Biosynthesis of the D2 cell adhesion molecule: pulse-chase studies in cultured fetal rat neuronal cells. J Cell Biol. 1984 Jun;98(6):2077–2081. doi: 10.1083/jcb.98.6.2077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mattei M. G., Baeteman M. A., Heilig R., Oberlé I., Davies K., Mandel J. L., Mattei J. F. Localization by in situ hybridization of the coagulation factor IX gene and of two polymorphic DNA probes with respect to the fragile X site. Hum Genet. 1985;69(4):327–331. doi: 10.1007/BF00291650. [DOI] [PubMed] [Google Scholar]
  14. Mattei M. G., Philip N., Passage E., Moisan J. P., Mandel J. L., Mattei J. F. DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome. Hum Genet. 1985;69(3):268–271. doi: 10.1007/BF00293038. [DOI] [PubMed] [Google Scholar]
  15. McClain D. A., Edelman G. M. A neural cell adhesion molecule from human brain. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6380–6384. doi: 10.1073/pnas.79.20.6380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Murray B. A., Hemperly J. J., Gallin W. J., MacGregor J. S., Edelman G. M., Cunningham B. A. Isolation of cDNA clones for the chicken neural cell adhesion molecule (N-CAM). Proc Natl Acad Sci U S A. 1984 Sep;81(17):5584–5588. doi: 10.1073/pnas.81.17.5584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rougon G., Deagostini-Bazin H., Hirn M., Goridis C. Tissue- and developmental stage-specific forms of a neural cell surface antigen linked to differences in glycosylation of a common polypeptide. EMBO J. 1982;1(10):1239–1244. doi: 10.1002/j.1460-2075.1982.tb00019.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rutishauser U. Molecular and biological properties of a neural cell adhesion molecule. Cold Spring Harb Symp Quant Biol. 1983;48(Pt 2):501–514. doi: 10.1101/sqb.1983.048.01.055. [DOI] [PubMed] [Google Scholar]
  19. Rutishauser U., Thiery J. P., Brackenbury R., Sela B. A., Edelman G. M. Mechanisms of adhesion among cells from neural tissues of the chick embryo. Proc Natl Acad Sci U S A. 1976 Feb;73(2):577–581. doi: 10.1073/pnas.73.2.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Williams A. F., Gagnon J. Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin. Science. 1982 May 14;216(4547):696–703. doi: 10.1126/science.6177036. [DOI] [PubMed] [Google Scholar]
  21. van den Elsen P., Bruns G., Gerhard D. S., Pravtcheva D., Jones C., Housman D., Ruddle F. A., Orkin S., Terhorst C. Assignment of the gene coding for the T3-delta subunit of the T3-T-cell receptor complex to the long arm of human chromosome 11 and to mouse chromosome 9. Proc Natl Acad Sci U S A. 1985 May;82(9):2920–2924. doi: 10.1073/pnas.82.9.2920. [DOI] [PMC free article] [PubMed] [Google Scholar]

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