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. 1988 Sep;170(9):4083–4090. doi: 10.1128/jb.170.9.4083-4090.1988

DNA sequence requirements for replication fork arrest at terC in Bacillus subtilis.

M T Smith 1, R G Wake 1
PMCID: PMC211412  PMID: 2842302

Abstract

The replication terminus, terC, of Bacillus subtilis is the chromosomal site at which movement of the clockwise replication fork is blocked. The effect of deletion or modification of DNA sequences on either side of terC (defined by the sequence location of the arrested clockwise fork junction) has been investigated. Deletion of sequences ahead of terC to within 250 base pairs (bp) had no effect on fork arrest, whereas removal of a further 130 bp abolished it. The 250-bp segment immediately ahead of terC encompassed the previously identified inverted repeat region as well as potential promoters for the transcription of an adjoining open reading frame (ORF). Deletion of DNA from the other side of terC up to 80 bp from it also abolished fork arrest. This deletion removed the bulk of the ORF. Disruption of this ORF by the insertion of 4 bp also abolished fork arrest. A model for clockwise fork arrest at terC, implicating both the inverted repeat region and the protein product of the ORF, is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bohannon D. E., Rosenkrantz M. S., Sonenshein A. L. Regulation of Bacillus subtilis glutamate synthase genes by the nitrogen source. J Bacteriol. 1985 Sep;163(3):957–964. doi: 10.1128/jb.163.3.957-964.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carrigan C. M., Haarsma J. A., Smith M. T., Wake R. G. Sequence features of the replication terminus of the Bacillus subtilis chromosome. Nucleic Acids Res. 1987 Oct 26;15(20):8501–8509. doi: 10.1093/nar/15.20.8501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ferrari F. A., Nguyen A., Lang D., Hoch J. A. Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol. 1983 Jun;154(3):1513–1515. doi: 10.1128/jb.154.3.1513-1515.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanley P. J., Carrigan C. M., Rowe D. B., Wake R. G. Breakdown and quantitation of the forked termination of replication intermediate of Bacillus subtilis. J Mol Biol. 1987 Aug 5;196(3):721–727. doi: 10.1016/0022-2836(87)90043-x. [DOI] [PubMed] [Google Scholar]
  5. Hill T. M., Henson J. M., Kuempel P. L. The terminus region of the Escherichia coli chromosome contains two separate loci that exhibit polar inhibition of replication. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1754–1758. doi: 10.1073/pnas.84.7.1754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hill T. M., Kopp B. J., Kuempel P. L. Termination of DNA replication in Escherichia coli requires a trans-acting factor. J Bacteriol. 1988 Feb;170(2):662–668. doi: 10.1128/jb.170.2.662-668.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Iismaa T. P., Carrigan C. M., Wake R. G. Relocation of the replication terminus, terC, of Bacillus subtilis to a new chromosomal site. Gene. 1988 Jul 30;67(2):183–191. doi: 10.1016/0378-1119(88)90395-2. [DOI] [PubMed] [Google Scholar]
  8. Iismaa T. P., Smith M. T., Wake R. G. Physical map of the Bacillus subtilis replication terminus region: its confirmation, extension and genetic orientation. Gene. 1984 Dec;32(1-2):171–180. doi: 10.1016/0378-1119(84)90045-3. [DOI] [PubMed] [Google Scholar]
  9. Iismaa T. P., Wake R. G. The normal replication terminus of the Bacillus subtilis chromosome, terC, is dispensable for vegetative growth and sporulation. J Mol Biol. 1987 May 20;195(2):299–310. doi: 10.1016/0022-2836(87)90651-6. [DOI] [PubMed] [Google Scholar]
  10. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kennett R. H., Sueoka N. Gene expression during outgrowth of Bacillus subtilis spores. The relationship between gene order on the chromosome and temporal sequence of enzyme synthesis. J Mol Biol. 1971 Aug 28;60(1):31–44. doi: 10.1016/0022-2836(71)90445-1. [DOI] [PubMed] [Google Scholar]
  12. Monteiro M. J., Sargent M. G., Piggot P. J. Characterization of the replication terminus of the Bacillus subtilis chromosome. J Gen Microbiol. 1984 Sep;130(9):2403–2414. doi: 10.1099/00221287-130-9-2403. [DOI] [PubMed] [Google Scholar]
  13. O'Sullivan M. A., Anagnostopoulos C. Replication terminus of the Bacillus subtilis chromosome. J Bacteriol. 1982 Jul;151(1):135–143. doi: 10.1128/jb.151.1.135-143.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schneider A. M., Gaisne M., Anagnostopoulos C. Genetic structure and internal rearrangements of stable merodiploids from Bacillus subtilis strains carrying the trpE26 mutation. Genetics. 1982 Jun;101(2):189–210. doi: 10.1093/genetics/101.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith M. T., Aynsley C., Wake R. G. Cloning and localization of the Bacillus subtilis chromosome replication terminus, terC. Gene. 1985;38(1-3):9–17. doi: 10.1016/0378-1119(85)90198-2. [DOI] [PubMed] [Google Scholar]
  16. Wallace R. B., Johnson M. J., Suggs S. V., Miyoshi K., Bhatt R., Itakura K. A set of synthetic oligodeoxyribonucleotide primers for DNA sequencing in the plasmid vector pBR322. Gene. 1981 Dec;16(1-3):21–26. doi: 10.1016/0378-1119(81)90057-3. [DOI] [PubMed] [Google Scholar]
  17. Weiss A. S., Hariharan I. K., Wake R. G. Analysis of the terminus region of the Bacillus subtilis chromosome. Nature. 1981 Oct 22;293(5834):673–675. doi: 10.1038/293673a0. [DOI] [PubMed] [Google Scholar]
  18. Weiss A. S., Smith M. T., Iismaa T. P., Wake R. G. Cloning DNA from the replication terminus region of the Bacillus subtilis chromosome. Gene. 1983 Sep;24(1):83–91. doi: 10.1016/0378-1119(83)90133-6. [DOI] [PubMed] [Google Scholar]
  19. Weiss A. S., Wake R. G. A unique DNA intermediate associated with termination of chromosome replication in Bacillus subtilis. Cell. 1984 Dec;39(3 Pt 2):683–689. doi: 10.1016/0092-8674(84)90475-6. [DOI] [PubMed] [Google Scholar]
  20. Weiss A. S., Wake R. G. Restriction map of DNA spanning the replication terminus of the Bacillus subtilis chromosome. J Mol Biol. 1983 Dec 5;171(2):119–137. doi: 10.1016/s0022-2836(83)80349-0. [DOI] [PubMed] [Google Scholar]
  21. Wilson G. A., Bott K. F. Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. J Bacteriol. 1968 Apr;95(4):1439–1449. doi: 10.1128/jb.95.4.1439-1449.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. de Massy B., Béjar S., Louarn J., Louarn J. M., Bouché J. P. Inhibition of replication forks exiting the terminus region of the Escherichia coli chromosome occurs at two loci separated by 5 min. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1759–1763. doi: 10.1073/pnas.84.7.1759. [DOI] [PMC free article] [PubMed] [Google Scholar]

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