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. 1987 Apr 1;104(4):865–874. doi: 10.1083/jcb.104.4.865

A mitotic form of the Golgi apparatus in HeLa cells

PMCID: PMC2114436  PMID: 3104351

Abstract

Galactosyltransferase, a marker for trans-Golgi cisternae in interphase cells, was localized in mitotic HeLa cells embedded in Lowicryl K4M by immunoelectron microscopy. Specific labeling was found only over multivesicular structures that we term Golgi clusters. Unlike Golgi stacks in interphase cells, these clusters lacked elongated cisternae and ordered stacking of their components but did comprise two distinct regions, one containing electron-lucent vesicles and the other, smaller, vesiculo-tubular structures. Labeling for galactosyltransferase was found predominantly over the latter region. Both structures were embedded in a dense matrix that excluded ribosomes and the cluster was often bounded by cisternae of the rough endoplasmic reticulum, sometimes on all sides. Clusters were present at all stages of mitosis examined, which included prometaphase, metaphase, and telophase. They were also identified in conventionally processed mitotic cells and shown to contain another trans-Golgi marker, thiamine pyrophosphatase. Serial sectioning showed that clusters were discrete and globular and multiple copies appeared to be dispersed in the cytoplasm. Their possible role in the division of the Golgi apparatus is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berlin R. D., Oliver J. M., Walter R. J. Surface functions during Mitosis I: phagocytosis, pinocytosis and mobility of surface-bound Con A. Cell. 1978 Oct;15(2):327–341. doi: 10.1016/0092-8674(78)90002-8. [DOI] [PubMed] [Google Scholar]
  2. Birky C. W., Jr The partitioning of cytoplasmic organelles at cell division. Int Rev Cytol Suppl. 1983;15:49–89. doi: 10.1016/b978-0-12-364376-6.50009-0. [DOI] [PubMed] [Google Scholar]
  3. Bretz R., Stäubli W. Detergent influence on rat-liver galactosyltransferase activities towards different acceptors. Eur J Biochem. 1977 Jul 1;77(1):181–192. doi: 10.1111/j.1432-1033.1977.tb11656.x. [DOI] [PubMed] [Google Scholar]
  4. Burke B., Griffiths G., Reggio H., Louvard D., Warren G. A monoclonal antibody against a 135-K Golgi membrane protein. EMBO J. 1982;1(12):1621–1628. doi: 10.1002/j.1460-2075.1982.tb01364.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dunphy W. G., Rothman J. E. Compartmental organization of the Golgi stack. Cell. 1985 Aug;42(1):13–21. doi: 10.1016/s0092-8674(85)80097-0. [DOI] [PubMed] [Google Scholar]
  6. Gerber A. C., Kozdrowski I., Wyss S. R., Berger E. G. The charge heterogeneity of soluble human galactosyltransferases isolated from milk, amniotic fluid and malignant ascites. Eur J Biochem. 1979 Feb 1;93(3):453–460. doi: 10.1111/j.1432-1033.1979.tb12843.x. [DOI] [PubMed] [Google Scholar]
  7. Green J., Griffiths G., Louvard D., Quinn P., Warren G. Passage of viral membrane proteins through the Golgi complex. J Mol Biol. 1981 Nov 15;152(4):663–698. doi: 10.1016/0022-2836(81)90122-4. [DOI] [PubMed] [Google Scholar]
  8. Griffiths G., Pfeiffer S., Simons K., Matlin K. Exit of newly synthesized membrane proteins from the trans cisterna of the Golgi complex to the plasma membrane. J Cell Biol. 1985 Sep;101(3):949–964. doi: 10.1083/jcb.101.3.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hiller G., Weber K. Golgi detection in mitotic and interphase cells by antibodies to secreted galactosyltransferase. Exp Cell Res. 1982 Nov;142(1):85–94. doi: 10.1016/0014-4827(82)90412-8. [DOI] [PubMed] [Google Scholar]
  10. Klevecz R. R. Automated cell cycle analysis. Methods Cell Biol. 1975;10:157–172. doi: 10.1016/s0091-679x(08)60735-9. [DOI] [PubMed] [Google Scholar]
  11. Louvard D., Reggio H., Warren G. Antibodies to the Golgi complex and the rough endoplasmic reticulum. J Cell Biol. 1982 Jan;92(1):92–107. doi: 10.1083/jcb.92.1.92. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lucocq J. M., Baschong W. Preparation of protein colloidal gold complexes in the presence of commonly used buffers. Eur J Cell Biol. 1986 Dec;42(2):332–337. [PubMed] [Google Scholar]
  13. Maul G. G., Brinkley B. R. The golgi apparatus during mitosis in human melanoma cells in vitro. Cancer Res. 1970 Sep;30(9):2326–2335. [PubMed] [Google Scholar]
  14. Melmed R. N., Benitez C. J., Holt S. J. An ultrastructural study of the pancreatic acinar cell in mitosis, with special reference to changes in the Golgi complex. J Cell Sci. 1973 Jan;12(1):163–173. doi: 10.1242/jcs.12.1.163. [DOI] [PubMed] [Google Scholar]
  15. Mollenhauer H. H., Morré D. J. Structural compartmentation of the cytosol: zones of exclusion, zones of adhesion, cytoskeletal and intercisternal elements. Subcell Biochem. 1978;5:327–359. doi: 10.1007/978-1-4615-7942-7_7. [DOI] [PubMed] [Google Scholar]
  16. Mort A. J., Lamport D. T. Anhydrous hydrogen fluoride deglycosylates glycoproteins. Anal Biochem. 1977 Oct;82(2):289–309. doi: 10.1016/0003-2697(77)90165-8. [DOI] [PubMed] [Google Scholar]
  17. Moskalewski S., Thyberg J., Hinek A., Friberg U. Fine structure of the golgi complex during mitosis of cartilaginous cells in vitro. Tissue Cell. 1977;9(2):185–196. doi: 10.1016/0040-8166(77)90015-5. [DOI] [PubMed] [Google Scholar]
  18. NOVIKOFF A. B., GOLDFISCHER S. Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proc Natl Acad Sci U S A. 1961 Jun 15;47:802–810. doi: 10.1073/pnas.47.6.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Novikoff P. M., Novikoff A. B., Quintana N., Hauw J. J. Golgi apparatus, GERL, and lysosomes of neurons in rat dorsal root ganglia, studied by thick section and thin section cytochemistry. J Cell Biol. 1971 Sep;50(3):859–886. doi: 10.1083/jcb.50.3.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Novikoff P. M., Touster O., Novikoff A. B., Tulsiani D. P. Effects of swainsonine on rat liver and kidney: biochemical and morphological studies. J Cell Biol. 1985 Aug;101(2):339–349. doi: 10.1083/jcb.101.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Peterson G. L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Biochem. 1977 Dec;83(2):346–356. doi: 10.1016/0003-2697(77)90043-4. [DOI] [PubMed] [Google Scholar]
  22. ROBBINS E., GONATAS N. K. THE ULTRASTRUCTURE OF A MAMMALIAN CELL DURING THE MITOTIC CYCLE. J Cell Biol. 1964 Jun;21:429–463. doi: 10.1083/jcb.21.3.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rambourg A., Clermont Y., Hermo L. Three-dimensional architecture of the golgi apparatus in Sertoli cells of the rat. Am J Anat. 1979 Apr;154(4):455–476. doi: 10.1002/aja.1001540402. [DOI] [PubMed] [Google Scholar]
  24. Rambourg A., Clermont Y., Hermo L. Three-dimensional structure of the Golgi apparatus. Methods Cell Biol. 1981;23:155–166. doi: 10.1016/s0091-679x(08)61497-1. [DOI] [PubMed] [Google Scholar]
  25. Rambourg A., Clermont Y., Marraud A. Three-dimensional structure of the osmium-impregnated Golgi apparatus as seen in the high voltage electron microscope. Am J Anat. 1974 May;140(1):27–45. doi: 10.1002/aja.1001400103. [DOI] [PubMed] [Google Scholar]
  26. Roth J., Bendayan M., Carlemalm E., Villiger W., Garavito M. Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem. 1981 May;29(5):663–671. doi: 10.1177/29.5.6166664. [DOI] [PubMed] [Google Scholar]
  27. Roth J., Berger E. G. Immunocytochemical localization of galactosyltransferase in HeLa cells: codistribution with thiamine pyrophosphatase in trans-Golgi cisternae. J Cell Biol. 1982 Apr;93(1):223–229. doi: 10.1083/jcb.93.1.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Roth J., Lentze M. J., Berger E. G. Immunocytochemical demonstration of ecto-galactosyltransferase in absorptive intestinal cells. J Cell Biol. 1985 Jan;100(1):118–125. doi: 10.1083/jcb.100.1.118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Schachter H., Jabbal I., Hudgin R. L., Pinteric L., McGuire E. J., Roseman S. Intracellular localization of liver sugar nucleotide glycoprotein glycosyltransferases in a Golgi-rich fraction. J Biol Chem. 1970 Mar 10;245(5):1090–1100. [PubMed] [Google Scholar]
  30. Schroeter D., Ehemann V., Paweletz N. Cellular compartments in mitotic cells: ultrahistochemical identification of Golgi elements in PtK-1 cells. Biol Cell. 1985;53(2):155–163. doi: 10.1111/j.1768-322x.1985.tb00363.x. [DOI] [PubMed] [Google Scholar]
  31. Slot J. W., Geuze H. J. A new method of preparing gold probes for multiple-labeling cytochemistry. Eur J Cell Biol. 1985 Jul;38(1):87–93. [PubMed] [Google Scholar]
  32. Strous G. J., Berger E. G. Biosynthesis, intracellular transport, and release of the Golgi enzyme galactosyltransferase (lactose synthetase A protein) in HeLa cells. J Biol Chem. 1982 Jul 10;257(13):7623–7628. [PubMed] [Google Scholar]
  33. Zeligs J. D., Wollman S. H. Mitosis in rat thyroid epithelial cells in vivo. I. Ultrastructural changes in cytoplasmic organelles during the mitotic cycle. J Ultrastruct Res. 1979 Jan;66(1):53–77. doi: 10.1016/s0022-5320(79)80065-9. [DOI] [PubMed] [Google Scholar]
  34. Zieve G. W., Turnbull D., Mullins J. M., McIntosh J. R. Production of large numbers of mitotic mammalian cells by use of the reversible microtubule inhibitor nocodazole. Nocodazole accumulated mitotic cells. Exp Cell Res. 1980 Apr;126(2):397–405. doi: 10.1016/0014-4827(80)90279-7. [DOI] [PubMed] [Google Scholar]

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