Skip to main content
NCBI home page
The Journal of Cell Biology logoLink to The Journal of Cell Biology
. 1987 Mar 1;104(3):725–731. doi: 10.1083/jcb.104.3.725

Receptors involved in lymphocyte homing: relationship between a carbohydrate-binding receptor and the MEL-14 antigen

PMCID: PMC2114538  PMID: 2950122

Abstract

Blood-borne lymphocytes extravasate in large numbers within peripheral lymph nodes (PN) and other secondary lymphoid organs. It has been proposed that the initiation of extravasation is based upon a family of cell adhesion molecules (homing receptors) that mediate lymphocyte attachment to specialized high endothelial venules (HEV) within the lymphoid tissues. A putative homing receptor has been identified by the monoclonal antibody, MEL-14, which recognizes an 80-90-kD glycoprotein on the surface of mouse lymphocytes and blocks the attachment of lymphocytes to PN HEV. In a companion study we characterize a carbohydrate-binding receptor on the surface of mouse lymphocytes that also appears to be involved in the interaction of lymphocytes with PN HEV. This receptor selectively binds to fluorescent beads derivatized with PPME, a polysaccharide rich in mannose-6-phosphate. In this report we examine the relationship between this carbohydrate-binding receptor and the putative homing receptor identified by the MEL-14 antibody. We found that: MEL-14 completely and selectively blocks the activity of the carbohydrate-binding receptor on mouse lymphocytes; the ability of six lymphoma cell lines to bind PPME beads correlates with cell-surface expression of the MEL-14 antigen, as well as PN HEV-binding activity; selection of lymphoma cell line variants for PPME-bead binding by fluorescence-activated cell sorting (FACS) produces highly correlated (r = 0.974, P less than 0.001) and selective changes in MEL-14 antigen expression. These results show that the carbohydrate-binding receptor on lymphocytes and the MEL-14 antigen, which have been independently implicated as receptors involved in PN-specific HEV attachment, are very closely related, if not identical, molecules.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahuja K. K. Fertilization studies in the hamster. The role of cell-surface carbohydrates. Exp Cell Res. 1982 Aug;140(2):353–362. doi: 10.1016/0014-4827(82)90124-0. [DOI] [PubMed] [Google Scholar]
  2. Bolwell G. P., Callow J. A., Evans L. V. Fertilization in brown algae. III. Preliminary characterization of putative gamete receptors from eggs and sperm of Fucus serratus. J Cell Sci. 1980 Jun;43:209–224. doi: 10.1242/jcs.43.1.209. [DOI] [PubMed] [Google Scholar]
  3. Butcher E. C., Scollay R. G., Weissman I. L. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur J Immunol. 1980 Jul;10(7):556–561. doi: 10.1002/eji.1830100713. [DOI] [PubMed] [Google Scholar]
  4. Chin Y. H., Carey G. D., Woodruff J. J. Lymphocyte recognition of lymph node high endothelium. I. Inhibition of in vitro binding by a component of thoracic duct lymph. J Immunol. 1980 Oct;125(4):1764–1769. [PubMed] [Google Scholar]
  5. Chin Y. H., Carey G. D., Woodruff J. J. Lymphocyte recognition of lymph node high endothelium. IV. Cell surface structures mediating entry into lymph nodes. J Immunol. 1982 Nov;129(5):1911–1915. [PubMed] [Google Scholar]
  6. Chin Y. H., Rasmussen R., Cakiroglu A. G., Woodruff J. J. Lymphocyte recognition of lymph node high endothelium. VI. Evidence of distinct structures mediating binding to high endothelial cells of lymph nodes and Peyer's patches. J Immunol. 1984 Dec;133(6):2961–2965. [PubMed] [Google Scholar]
  7. Dailey M. O., Fathman C. G., Butcher E. C., Pillemer E., Weissman I. Abnormal migration of T lymphocyte clones. J Immunol. 1982 May;128(5):2134–2136. [PubMed] [Google Scholar]
  8. Davignon D., Martz E., Reynolds T., Kürzinger K., Springer T. A. Monoclonal antibody to a novel lymphocyte function-associated antigen (LFA-1): mechanism of blockade of T lymphocyte-mediated killing and effects on other T and B lymphocyte functions. J Immunol. 1981 Aug;127(2):590–595. [PubMed] [Google Scholar]
  9. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  10. Gallatin M., St John T. P., Siegelman M., Reichert R., Butcher E. C., Weissman I. L. Lymphocyte homing receptors. Cell. 1986 Mar 14;44(5):673–680. doi: 10.1016/0092-8674(86)90832-9. [DOI] [PubMed] [Google Scholar]
  11. Glabe C. G., Grabel L. B., Vacquier V. D., Rosen S. D. Carbohydrate specificity of sea urchin sperm bindin: a cell surface lectin mediating sperm-egg adhesion. J Cell Biol. 1982 Jul;94(1):123–128. doi: 10.1083/jcb.94.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Glabe C. G., Yednock T., Rosen S. D. Reversible disruption of cultured endothelial monolayers by sulphated fucans. J Cell Sci. 1983 May;61:475–490. doi: 10.1242/jcs.61.1.475. [DOI] [PubMed] [Google Scholar]
  13. Grabel L. B., Singer M. S., Martin G. R., Rosen S. D. Teratocarcinoma stem cell adhesion: the role of divalent cations and a cell surface lectin. J Cell Biol. 1983 Jun;96(6):1532–1537. doi: 10.1083/jcb.96.6.1532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaplan A., Fischer D., Sly W. S. Correlation of structural features of phosphomannans with their ability to inhibit pinocytosis of human beta-glucuronidase by human fibroblasts. J Biol Chem. 1978 Feb 10;253(3):647–650. [PubMed] [Google Scholar]
  15. MARCHESI V. T., GOWANS J. L. THE MIGRATION OF LYMPHOCYTES THROUGH THE ENDOTHELIUM OF VENULES IN LYMPH NODES: AN ELECTRON MICROSCOPE STUDY. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:283–290. doi: 10.1098/rspb.1964.0002. [DOI] [PubMed] [Google Scholar]
  16. Rasmussen R. A., Chin Y. H., Woodruff J. J., Easton T. G. Lymphocyte recognition of lymph node high endothelium. VII. Cell surface proteins involved in adhesion defined by monoclonal anti-HEBFLN (A.11) antibody. J Immunol. 1985 Jul;135(1):19–24. [PubMed] [Google Scholar]
  17. Reichert R. A., Gallatin W. M., Weissman I. L., Butcher E. C. Germinal center B cells lack homing receptors necessary for normal lymphocyte recirculation. J Exp Med. 1983 Mar 1;157(3):813–827. doi: 10.1084/jem.157.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Roberts D. D., Haverstick D. M., Dixit V. M., Frazier W. A., Santoro S. A., Ginsburg V. The platelet glycoprotein thrombospondin binds specifically to sulfated glycolipids. J Biol Chem. 1985 Aug 5;260(16):9405–9411. [PubMed] [Google Scholar]
  19. Roberts D. D., Williams S. B., Gralnick H. R., Ginsburg V. von Willebrand factor binds specifically to sulfated glycolipids. J Biol Chem. 1986 Mar 5;261(7):3306–3309. [PubMed] [Google Scholar]
  20. Sarmiento M., Dialynas D. P., Lancki D. W., Wall K. A., Lorber M. I., Loken M. R., Fitch F. W. Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis. Immunol Rev. 1982;68:135–169. doi: 10.1111/j.1600-065x.1982.tb01063.x. [DOI] [PubMed] [Google Scholar]
  21. Siegelman M., Bond M. W., Gallatin W. M., St John T., Smith H. T., Fried V. A., Weissman I. L. Cell surface molecule associated with lymphocyte homing is a ubiquitinated branched-chain glycoprotein. Science. 1986 Feb 21;231(4740):823–829. doi: 10.1126/science.3003913. [DOI] [PubMed] [Google Scholar]
  22. Slodki M. E., Ward R. M., Boundy J. A. Concanavalin A as a probe of phosphomannan molecular structure. Biochim Biophys Acta. 1973 Apr 28;304(2):449–456. doi: 10.1016/0304-4165(73)90264-x. [DOI] [PubMed] [Google Scholar]
  23. Springer T. A., Davignon D., Ho M. K., Kürzinger K., Martz E., Sanchez-Madrid F. LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function. Immunol Rev. 1982;68:171–195. doi: 10.1111/j.1600-065x.1982.tb01064.x. [DOI] [PubMed] [Google Scholar]
  24. Stamper H. B., Jr, Woodruff J. J. Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules. J Exp Med. 1976 Sep 1;144(3):828–833. doi: 10.1084/jem.144.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stevens S. K., Weissman I. L., Butcher E. C. Differences in the migration of B and T lymphocytes: organ-selective localization in vivo and the role of lymphocyte-endothelial cell recognition. J Immunol. 1982 Feb;128(2):844–851. [PubMed] [Google Scholar]
  26. Stoolman L. M., Rosen S. D. Possible role for cell-surface carbohydrate-binding molecules in lymphocyte recirculation. J Cell Biol. 1983 Mar;96(3):722–729. doi: 10.1083/jcb.96.3.722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stoolman L. M., Tenforde T. S., Rosen S. D. Phosphomannosyl receptors may participate in the adhesive interaction between lymphocytes and high endothelial venules. J Cell Biol. 1984 Oct;99(4 Pt 1):1535–1540. doi: 10.1083/jcb.99.4.1535. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Töpfer-Petersen E., Friess A. E., Nguyen H., Schill W. B. Evidence for a fucose-binding protein in boar spermatozoa. Histochemistry. 1985;83(2):139–145. doi: 10.1007/BF00495144. [DOI] [PubMed] [Google Scholar]
  29. Yednock T. A., Stoolman L. M., Rosen S. D. Phosphomannosyl-derivatized beads detect a receptor involved in lymphocyte homing. J Cell Biol. 1987 Mar;104(3):713–723. doi: 10.1083/jcb.104.3.713. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Cell Biology are provided here courtesy of The Rockefeller University Press

RESOURCES