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. 1988 Sep;170(9):4379–4381. doi: 10.1128/jb.170.9.4379-4381.1988

Activation of recF-dependent recombination in Escherichia coli by bacteriophage lambda- and P22-encoded functions.

A R Poteete 1, M R Volkert 1
PMCID: PMC211454  PMID: 2842317

Abstract

Escherichia coli strains bearing wild-type and mutant alleles of various recombination genes, as well as plasmids that express recombination-related genes of bacteriophages lambda and P22, were tested for their proficiency as recipients in Hfr-mediated conjugation. It was found that the homologous recombination systems of both phages could promote recombination in a recB recC mutant host. In addition, the Abc function of P22, but not the Gam function of lambda, was found to inhibit recombination in a wild-type host; however, both Abc and Gam inhibited recombination in a recF mutant host. These observations are interpreted as indicating that the recombination systems of both phages, as well as the RecBCD-modulating functions Abc and Gam, all activate the RecF recombination pathway of E. coli.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armengod M. E. Role of the recF gene of Escherichia coli K-12 in lambda recombination. Mol Gen Genet. 1981;181(4):497–504. doi: 10.1007/BF00428742. [DOI] [PubMed] [Google Scholar]
  2. Botstein D., Matz M. J. A recombination function essential to the growth of bacteriophage P22. J Mol Biol. 1970 Dec 28;54(3):417–440. doi: 10.1016/0022-2836(70)90119-1. [DOI] [PubMed] [Google Scholar]
  3. Clark A. J. Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet. 1973;7:67–86. doi: 10.1146/annurev.ge.07.120173.000435. [DOI] [PubMed] [Google Scholar]
  4. Echolas H., Gingery R. Mutants of bacteriophage lambda defective in vegetative genetic recombination. J Mol Biol. 1968 Jul 14;34(2):239–249. doi: 10.1016/0022-2836(68)90249-0. [DOI] [PubMed] [Google Scholar]
  5. Fenton A. C., Poteete A. R. Genetic analysis of the erf region of the bacteriophage P22 chromosome. Virology. 1984 Apr 15;134(1):148–160. doi: 10.1016/0042-6822(84)90280-0. [DOI] [PubMed] [Google Scholar]
  6. Friedman S. A., Hays J. B. Selective inhibition of Escherichia coli recBC activities by plasmid-encoded GamS function of phage lambda. Gene. 1986;43(3):255–263. doi: 10.1016/0378-1119(86)90214-3. [DOI] [PubMed] [Google Scholar]
  7. Gillen J. R., Willis D. K., Clark A. J. Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. J Bacteriol. 1981 Jan;145(1):521–532. doi: 10.1128/jb.145.1.521-532.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hays J. B., Smith T. A., Friedman S. A., Lee E., Coffman G. L. RecF and RecBC function during recombination of nonreplicating, UV-irradiated phage lambda DNA and during other recombination processes. Cold Spring Harb Symp Quant Biol. 1984;49:475–483. doi: 10.1101/sqb.1984.049.01.054. [DOI] [PubMed] [Google Scholar]
  9. Karu A. E., Sakaki Y., Echols H., Linn S. The gamma protein specified by bacteriophage gamma. Structure and inhibitory activity for the recBC enzyme of Escherichia coli. J Biol Chem. 1975 Sep 25;250(18):7377–7387. [PubMed] [Google Scholar]
  10. Murphy K. C., Fenton A. C., Poteete A. R. Sequence of the bacteriophage P22 anti-recBCD (abc) genes and properties of P22 abc region deletion mutants. Virology. 1987 Oct;160(2):456–464. doi: 10.1016/0042-6822(87)90017-1. [DOI] [PubMed] [Google Scholar]
  11. Poteete A. R., Fenton A. C. Lambda red-dependent growth and recombination of phage P22. Virology. 1984 Apr 15;134(1):161–167. doi: 10.1016/0042-6822(84)90281-2. [DOI] [PubMed] [Google Scholar]
  12. Poteete A. R., Fenton A. C., Murphy K. C. Modulation of Escherichia coli RecBCD activity by the bacteriophage lambda Gam and P22 Abc functions. J Bacteriol. 1988 May;170(5):2012–2021. doi: 10.1128/jb.170.5.2012-2021.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Signer E. R., Weil J. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination. J Mol Biol. 1968 Jul 14;34(2):261–271. doi: 10.1016/0022-2836(68)90251-9. [DOI] [PubMed] [Google Scholar]
  14. Stahl F. W., McMilin K. D., Stahl M. M., Crasemann J. M., Lam S. The distribution of crossovers along unreplicated lambda bacteriophage chromosomes. Genetics. 1974 Jul;77(3):395–408. doi: 10.1093/genetics/77.3.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  16. Zagursky R. J., Hays J. B. Expression of the phage lambda recombination genes exo and bet under lacPO control on a multi-copy plasmid. Gene. 1983 Sep;23(3):277–292. doi: 10.1016/0378-1119(83)90018-5. [DOI] [PubMed] [Google Scholar]

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