Abstract
Schwann cell proliferation induced by a myelin-enriched fraction was examined in vitro. Although nearly all the Schwann cells contained material that was recognized by antisera to myelin basic protein after 24 h, only 1% of the cells were synthesizing DNA. 72 h after the addition of the mitogen a maximum of 10% of the cells incorporated [3H]thymidine. If the cultures were treated with the myelin-enriched fraction for 24 h and then washed, the number of proliferating Schwann cells decreased by 75% when compared with those cells that were incubated with the mitogen continuously. When Schwann cells were labeled with [14C]thymidine followed by a pulse of [3H]thymidine 24 h later, every Schwann cell labeled with [3H]thymidine was also labeled with [14C]thymidine. Although almost every Schwann cell can metabolize the myelin membranes within 24 h of exposure, a small population of cell initially utilizes the myelin as a mitogen, and this population continues to divide only if myelin is present in the extracellular media. The percentage of the Schwann cells that initially recognize the myelin-enriched fraction as a mitogen is dependent upon the age of the animal from which the cells were prepared.
Full Text
The Full Text of this article is available as a PDF (1.6 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Beuche W., Friede R. L. The role of non-resident cells in Wallerian degeneration. J Neurocytol. 1984 Oct;13(5):767–796. doi: 10.1007/BF01148493. [DOI] [PubMed] [Google Scholar]
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
- Bradley W. G., Asbury A. K. Duration of synthesis phase in neuilemma cells in mouse sciatic nerve during degeneration. Exp Neurol. 1970 Feb;26(2):275–282. doi: 10.1016/0014-4886(70)90125-1. [DOI] [PubMed] [Google Scholar]
- Brockes J. P., Fields K. L., Raff M. C. Studies on cultured rat Schwann cells. I. Establishment of purified populations from cultures of peripheral nerve. Brain Res. 1979 Apr 6;165(1):105–118. doi: 10.1016/0006-8993(79)90048-9. [DOI] [PubMed] [Google Scholar]
- Cassel D., Wood P. M., Bunge R. P., Glaser L. Mitogenicity of brain axolemma membranes and soluble factors for dorsal root ganglion Schwann cells. J Cell Biochem. 1982;18(4):433–445. doi: 10.1002/jcb.1982.240180405. [DOI] [PubMed] [Google Scholar]
- DeVries G. H., Minier L. N., Lewis B. L. Further studies on the mitogenic response of cultured Schwann cells to rat CNS axolemma-enriched fractions. Brain Res. 1983 Jul;285(1):87–93. doi: 10.1016/0165-3806(83)90112-8. [DOI] [PubMed] [Google Scholar]
- DeVries G. H., Salzer J. L., Bunge R. P. Axolemma-enriched fractions isolated from PNS and CNS are mitogenic for cultured Schwann cells. Brain Res. 1982 Feb;255(2):295–299. doi: 10.1016/0165-3806(82)90028-1. [DOI] [PubMed] [Google Scholar]
- Meador-Woodruff J. H., Yoshino J. E., Bigbee J. W., Lewis B. L., Devries G. H. Differential proliferative responses of cultured Schwann cells to axolemma and myelin-enriched fractions. II. Morphological studies. J Neurocytol. 1985 Aug;14(4):619–635. doi: 10.1007/BF01200801. [DOI] [PubMed] [Google Scholar]
- Mirsky R., Winter J., Abney E. R., Pruss R. M., Gavrilovic J., Raff M. C. Myelin-specific proteins and glycolipids in rat Schwann cells and oligodendrocytes in culture. J Cell Biol. 1980 Mar;84(3):483–494. doi: 10.1083/jcb.84.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakane P. K., Pierce G. B., Jr Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens. J Cell Biol. 1967 May;33(2):307–318. doi: 10.1083/jcb.33.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raff M. C., Abney E., Brockes J. P., Hornby-Smith A. Schwann cell growth factors. Cell. 1978 Nov;15(3):813–822. doi: 10.1016/0092-8674(78)90266-0. [DOI] [PubMed] [Google Scholar]
- Salzer J. L., Bunge R. P. Studies of Schwann cell proliferation. I. An analysis in tissue culture of proliferation during development, Wallerian degeneration, and direct injury. J Cell Biol. 1980 Mar;84(3):739–752. doi: 10.1083/jcb.84.3.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yoshino J. E., Dinneen M. P., Lewis B. L., Meador-Woodruff J. H., Devries G. H. Differential proliferative responses of cultured Schwann cells to axolemma- and myelin-enriched fractions. I. Biochemical studies. J Cell Biol. 1984 Dec;99(6):2309–2313. doi: 10.1083/jcb.99.6.2309. [DOI] [PMC free article] [PubMed] [Google Scholar]