Abstract
The nucleotide sequence at the oriT region of the IncI1 plasmid R64 was determined. A recombinant plasmid carrying a 141-base-pair R64 sequence was mobilized with a normal frequency, while a plasmid carrying only 44 base pairs of this R64 sequence was mobilized with a frequency 1/10 that of the original plasmid. The oriT region of the R64 plasmid contains two inverted-repeat sequences.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brasch M. A., Meyer R. J. Genetic organization of plasmid R1162 DNA involved in conjugative mobilization. J Bacteriol. 1986 Aug;167(2):703–710. doi: 10.1128/jb.167.2.703-710.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Derbyshire K. M., Willetts N. S. Mobilization of the non-conjugative plasmid RSF1010: a genetic analysis of its origin of transfer. Mol Gen Genet. 1987 Jan;206(1):154–160. doi: 10.1007/BF00326551. [DOI] [PubMed] [Google Scholar]
- Furuichi T., Komano T., Nisioka T. Physical and genetic analyses of the Inc-I alpha plasmid R64. J Bacteriol. 1984 Jun;158(3):997–1004. doi: 10.1128/jb.158.3.997-1004.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartskeerl R. A., vd Guchte M., Zuidweg E. M., Hoekstra W. P. Physical and genetic characterization of the IncI plasmid R144-drd3. Plasmid. 1984 Nov;12(3):215–217. doi: 10.1016/0147-619x(84)90048-9. [DOI] [PubMed] [Google Scholar]
- Hartskeerl R., Tommassen J., Hoekstra W. Relationship between the proteins encoded by the exclusion determining locus of the IncI plasmid R144 and the cellular localization of these proteins in Escherichia coli K-12. Mol Gen Genet. 1985;200(1):138–144. doi: 10.1007/BF00383327. [DOI] [PubMed] [Google Scholar]
- Komano T., Kim S. R., Nisioka T. Distribution of shufflon among IncI plasmids. J Bacteriol. 1987 Nov;169(11):5317–5319. doi: 10.1128/jb.169.11.5317-5319.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komano T., Kubo A., Kayanuma T., Furuichi T., Nisioka T. Highly mobile DNA segment of IncI alpha plasmid R64: a clustered inversion region. J Bacteriol. 1986 Jan;165(1):94–100. doi: 10.1128/jb.165.1.94-100.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Komano T., Kubo A., Nisioka T. Shufflon: multi-inversion of four contiguous DNA segments of plasmid R64 creates seven different open reading frames. Nucleic Acids Res. 1987 Feb 11;15(3):1165–1172. doi: 10.1093/nar/15.3.1165. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merryweather A., Rees C. E., Smith N. M., Wilkins B. M. Role of sog polypeptides specified by plasmid ColIb-P9 and their transfer between conjugating bacteria. EMBO J. 1986 Nov;5(11):3007–3012. doi: 10.1002/j.1460-2075.1986.tb04599.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oka A., Nomura N., Morita M., Sugisaki H., Sugimoto K., Takanami M. Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity. Mol Gen Genet. 1979 May 4;172(2):151–159. doi: 10.1007/BF00268276. [DOI] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson R., Taylor L., Kelly K., Everett R., Willetts N. The F plasmid origin of transfer: DNA sequence of wild-type and mutant origins and location of origin-specific nicks. EMBO J. 1984 May;3(5):1175–1180. doi: 10.1002/j.1460-2075.1984.tb01947.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
- Willetts N., Wilkins B. Processing of plasmid DNA during bacterial conjugation. Microbiol Rev. 1984 Mar;48(1):24–41. doi: 10.1128/mr.48.1.24-41.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]