Abstract
Type VII collagen is one of the newly identified members of the collagen family. A variety of evidence, including ultrastructural immunolocalization, has previously shown that type VII collagen is a major structural component of anchoring fibrils, found immediately beneath the lamina densa of many epithelia. In the present study, ultrastructural immunolocalization with monoclonal and monospecific polyclonal antibodies to type VII collagen and with a monoclonal antibody to type IV collagen indicates that amorphous electron-dense structures which we term "anchoring plaques" are normal features of the basement membrane zone of skin and cornea. These plaques contain type IV collagen and the carboxyl-terminal domain of type VII collagen. Banded anchoring fibrils extend from both the lamina densa and from these plaques, and can be seen bridging the plaques with the lamina densa and with other anchoring plaques. These observations lead to the postulation of a multilayered network of anchoring fibrils and anchoring plaques which underlies the basal lamina of several anchoring fibril-containing tissues. This extended network is capable of entrapping a large number of banded collagen fibers, microfibrils, and other stromal matrix components. These observations support the hypothesis that anchoring fibrils provide additional adhesion of the lamina densa to its underlying stroma.
Full Text
The Full Text of this article is available as a PDF (7.1 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bentz H., Morris N. P., Murray L. W., Sakai L. Y., Hollister D. W., Burgeson R. E. Isolation and partial characterization of a new human collagen with an extended triple-helical structural domain. Proc Natl Acad Sci U S A. 1983 Jun;80(11):3168–3172. doi: 10.1073/pnas.80.11.3168. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bruns R. R. A symmetrical, extracellular fibril. J Cell Biol. 1969 Aug;42(2):418–430. doi: 10.1083/jcb.42.2.418. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burgeson R. E., Morris N. P., Murray L. W., Duncan K. G., Keene D. R., Sakai L. Y. The structure of type VII collagen. Ann N Y Acad Sci. 1985;460:47–57. doi: 10.1111/j.1749-6632.1985.tb51156.x. [DOI] [PubMed] [Google Scholar]
- Dieringer H., Hollister D. W., Glanville R. W., Sakai L. Y., Kühn K. Structural studies of human basement-membrane collagen with the use of a monoclonal antibody. Biochem J. 1985 Apr 1;227(1):217–222. doi: 10.1042/bj2270217. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gipson I. K., Grill S. M., Spurr S. J., Brennan S. J. Hemidesmosome formation in vitro. J Cell Biol. 1983 Sep;97(3):849–857. doi: 10.1083/jcb.97.3.849. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldsmith L. A., Briggaman R. A. Monoclonal antibodies to anchoring fibrils for the diagnosis of epidermolysis bullosa. J Invest Dermatol. 1983 Nov;81(5):464–466. doi: 10.1111/1523-1747.ep12522669. [DOI] [PubMed] [Google Scholar]
- Hay E. D. Interaction of embryonic surface and cytoskeleton with extracellular matrix. Am J Anat. 1982 Sep;165(1):1–12. doi: 10.1002/aja.1001650102. [DOI] [PubMed] [Google Scholar]
- Hessle H., Sakai L. Y., Hollister D. W., Burgeson R. E., Engvall E. Basement membrane diversity detected by monoclonal antibodies. Differentiation. 1984;26(1):49–54. doi: 10.1111/j.1432-0436.1984.tb01372.x. [DOI] [PubMed] [Google Scholar]
- Kanwar Y. S., Farquhar M. G. Detachment of endothelium and epithelium from the glomerular basement membrane produced by kidney perfusion with neuraminidase. Lab Invest. 1980 Mar;42(3):375–384. [PubMed] [Google Scholar]
- Kawanami O., Ferrans V. J., Crystal R. G. Anchoring fibrils in the normal canine respiratory system. Am Rev Respir Dis. 1979 Sep;120(3):595–611. doi: 10.1164/arrd.1979.120.3.595. [DOI] [PubMed] [Google Scholar]
- Lunstrum G. P., Sakai L. Y., Keene D. R., Morris N. P., Burgeson R. E. Large complex globular domains of type VII procollagen contribute to the structure of anchoring fibrils. J Biol Chem. 1986 Jul 5;261(19):9042–9048. [PubMed] [Google Scholar]
- Morris N. P., Keene D. R., Glanville R. W., Bentz H., Burgeson R. E. The tissue form of type VII collagen is an antiparallel dimer. J Biol Chem. 1986 Apr 25;261(12):5638–5644. [PubMed] [Google Scholar]
- Palade G. E., Farquhar M. G. A special fibril of the dermis. J Cell Biol. 1965 Oct;27(1):215–224. doi: 10.1083/jcb.27.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakai L. Y., Engvall E., Hollister D. W., Burgeson R. E. Production and characterization of a monoclonal antibody to human Type IV collagen. Am J Pathol. 1982 Sep;108(3):310–318. [PMC free article] [PubMed] [Google Scholar]
- Sakai L. Y., Keene D. R., Engvall E. Fibrillin, a new 350-kD glycoprotein, is a component of extracellular microfibrils. J Cell Biol. 1986 Dec;103(6 Pt 1):2499–2509. doi: 10.1083/jcb.103.6.2499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sakai L. Y., Keene D. R., Morris N. P., Burgeson R. E. Type VII collagen is a major structural component of anchoring fibrils. J Cell Biol. 1986 Oct;103(4):1577–1586. doi: 10.1083/jcb.103.4.1577. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Susi F. R., Belt W. D., Kelly J. W. Fine structure of fibrillar complexes associated with the basement membrane in human oral mucosa. J Cell Biol. 1967 Aug;34(2):686–690. doi: 10.1083/jcb.34.2.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wasano K., Yamamoto T. Microthread-like filaments connecting the epithelial basal lamina with underlying fibrillar components of the connective tissue in the rat trachea. A real anchoring device? Cell Tissue Res. 1985;239(3):485–495. doi: 10.1007/BF00219226. [DOI] [PubMed] [Google Scholar]