Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Sep;170(9):4388–4391. doi: 10.1128/jb.170.9.4388-4391.1988

Conjugative plasmid transfer from Enterococcus faecalis to Escherichia coli.

P Trieu-Cuot 1, C Carlier 1, P Courvalin 1
PMCID: PMC211457  PMID: 3137216

Abstract

The possibility of transfer of genetic information by conjugation from gram-positive to gram-negative bacteria was investigated with a pBR322-pAM beta 1 chimeric plasmid, designated pAT191. This shuttle vector, which possesses the tra functions of the streptococcal plasmid pAM beta 1, was conjugatively transferred from Enterococcus faecalis to Escherichia coli with an average frequency of 5 x 10(-9) per donor colony formed after mating.

Full text

PDF
4388

Images in this article

4390Image
on p.4390

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arthur M., Andremont A., Courvalin P. Distribution of erythromycin esterase and rRNA methylase genes in members of the family Enterobacteriaceae highly resistant to erythromycin. Antimicrob Agents Chemother. 1987 Mar;31(3):404–409. doi: 10.1128/aac.31.3.404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brisson-Noël A., Arthur M., Courvalin P. Evidence for natural gene transfer from gram-positive cocci to Escherichia coli. J Bacteriol. 1988 Apr;170(4):1739–1745. doi: 10.1128/jb.170.4.1739-1745.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Courvalin P., Carlier C. Tn1545: a conjugative shuttle transposon. Mol Gen Genet. 1987 Feb;206(2):259–264. doi: 10.1007/BF00333582. [DOI] [PubMed] [Google Scholar]
  4. Courvalin P., Carlier C. Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol Gen Genet. 1986 Nov;205(2):291–297. doi: 10.1007/BF00430441. [DOI] [PubMed] [Google Scholar]
  5. Horinouchi S., Byeon W. H., Weisblum B. A complex attenuator regulates inducible resistance to macrolides, lincosamides, and streptogramin type B antibiotics in Streptococcus sanguis. J Bacteriol. 1983 Jun;154(3):1252–1262. doi: 10.1128/jb.154.3.1252-1262.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jacob A. E., Hobbs S. J. Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol. 1974 Feb;117(2):360–372. doi: 10.1128/jb.117.2.360-372.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kuramitsu H. K., Casadaban M. J. Transposition of the gram-positive transposon Tn917 in Escherichia coli. J Bacteriol. 1986 Aug;167(2):711–712. doi: 10.1128/jb.167.2.711-712.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LeBlanc D. J., Hassell F. P. Transformation of Streptococcus sanguis Challis by plasmid deoxyribonucleic acid from Streptococcus faecalis. J Bacteriol. 1976 Oct;128(1):347–355. doi: 10.1128/jb.128.1.347-355.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Leblanc D. J., Lee L. N. Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region. J Bacteriol. 1984 Feb;157(2):445–453. doi: 10.1128/jb.157.2.445-453.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martin B., Alloing G., Méjean V., Claverys J. P. Constitutive expression of erythromycin resistance mediated by the ermAM determinant of plasmid pAM beta 1 results from deletion of 5' leader peptide sequences. Plasmid. 1987 Nov;18(3):250–253. doi: 10.1016/0147-619x(87)90068-0. [DOI] [PubMed] [Google Scholar]
  11. Morse S. A., Johnson S. R., Biddle J. W., Roberts M. C. High-level tetracycline resistance in Neisseria gonorrhoeae is result of acquisition of streptococcal tetM determinant. Antimicrob Agents Chemother. 1986 Nov;30(5):664–670. doi: 10.1128/aac.30.5.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith M. D., Guild W. R. Improved method for conjugative transfer by filter mating of Streptococcus pneumoniae. J Bacteriol. 1980 Oct;144(1):457–459. doi: 10.1128/jb.144.1.457-459.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Trieu-Cuot P., Arthur M., Courvalin P. Origin, evolution and dissemination of antibiotic resistance genes. Microbiol Sci. 1987 Sep;4(9):263–266. [PubMed] [Google Scholar]
  14. Trieu-Cuot P., Courvalin P. Transposition behavior of IS15 and its progenitor IS15-delta: are cointegrates exclusive end products? Plasmid. 1985 Jul;14(1):80–89. doi: 10.1016/0147-619x(85)90034-4. [DOI] [PubMed] [Google Scholar]
  15. Trieu-Cuot P., Gerbaud G., Lambert T., Courvalin P. In vivo transfer of genetic information between gram-positive and gram-negative bacteria. EMBO J. 1985 Dec 16;4(13A):3583–3587. doi: 10.1002/j.1460-2075.1985.tb04120.x. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES